Detection method for obligate hydrocarbon oxidizing bacteria

A technology of hydrocarbon oxidizing bacteria and detection methods, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, which can solve the limitations of the practical application of obligate hydrocarbon oxidizing microorganisms, the inability to meet the requirements of actual production, and the complexity of experimental operation equipment and other problems, to achieve the effects of software-assisted counting fast and direct, shortening the training cycle, and significant economic benefits
CN102453746BActive Publication Date: 2013-11-06CHINA PETROLEUM & CHEM CORP +1
3 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA PETROLEUM & CHEM CORP
Publication Date
2013-11-06

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of applied microorganism detection methods, in particular to an obligate hydrocarbon-oxidizing bacteria detection method that provides microorganism detection means for surface microorganism exploration technology. Background technique

[0002] Microbial oil and gas exploration is gradually established based on the theory of vertical migration of volatile or semi-volatile hydrocarbons in underground oil and gas reservoirs. With the continuous supply of a certain concentration of hydrocarbons in the surface soil, the microorganisms in the oxidized layer that use hydrocarbons as the sole carbon source can grow and reproduce rapidly, and the difference in abundance can be used to indirectly represent the concentration of light hydrocarbons in the soil. Therefore, the determination of the number of oxidizing microorganisms (that is: obligate hydrocarbon oxidizing bacteria) that use hydrocarbons as the only carbo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More