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Electromagnetic safety analysis method for near field of plane phased control array antenna
ActiveCN102539940AHigh precisionGood adaptabilityAntenna radiation diagramsAntenna radiationElectric field
Owner:CHINA SHIP DEV & DESIGN CENT
Detection method for obligate hydrocarbon oxidizing bacteria
ActiveCN102453746AEasy to operateShort training periodMicrobiological testing/measurementColor changesShort terms
The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.
Owner:CHINA PETROLEUM & CHEM CORP +1
Method for detecting pollution toxicity of soil by adopting electric signals of double-chamber microbial fuel cell
InactiveCN103645231ALow costLow running costMaterial analysis by electric/magnetic meansPotassium ferricyanidePollution
The invention discloses a method for detecting the pollution toxicity of soil by adopting electric signals of a double-chamber microbial fuel cell and belongs to the technical field of detection of soil pollution. According to the method, electric quantity generated by decomposing organic matters through soil microorganisms is used as an index for detecting the pollution toxicity of the soil; compared with a traditional method for detecting the toxicity by microorganism activity, biomass and the like, the method has the advantages of low cost, easiness in operation and short detection period. The double-chamber microbial fuel cell used in the method comprises an anode, a cathode, an anode fixing groove, a cathode fixing groove, an anode chamber, a cathode chamber, a top cover of the anode chamber, a top cover of the cathode chamber, a diaphragm, a rubber cushion, a lead wire, a load, bolts and nuts. Before the double-chamber microbial fuel cell is operated, polluted soil and glucose are mixed and are added into the anode chamber; a potassium ferricyanide solution is introduced into the cathode chamber to be used as an electron acceptor; after the double-chamber microbial fuel cell is operated for 48 hours, the generated electric quantity in the 48 hours is calculated and is used for evaluating the toxicity of pollutants. The method for detecting the pollution toxicity of the soil by adopting the electric signals of the double-chamber microbial fuel cell has high scientific values and application values.
Owner:NANJING NORMAL UNIVERSITY
Kit for jointly detecting breast cancer 21 genes and preparation method of kit
InactiveCN104004844AEasy to operateShorten detection experiment timeMicrobiological testing/measurementSingle sampleBAG1
The invention discloses a kit for jointly detecting breast cancer 21 genes. A PCR (polymerase chain reaction) amplification primer consists of 21 primer pairs, and can be used for jointly detecting 21 gene-related expression quantities: Ki67, STK15, Survivin, CCNB1, MYBL2, MMP11, CTSL2, GRB7, HER2, GSTM1, CD68, BAG1, ER, PGR, BCL2, SCUBE2, ACTB, GAPDH, PRLPO, GUS, and TFRC. Output fragments of the 21 pairs of primers related in the invention are smaller than 100bp, so that PCR efficiency after reverse transcription of mRNA (messenger ribonucleic acid) extracted by a paraffin specimen is greatly improved, all primers of 21 genes are premixed, and time and labor for designing and synthesizing 21 pairs of genes by an experimenter are saved; the corresponding primers of the 21 genes are not needed to be added for premixing in mother liquor, and therefore, errors of reaction holes are greatly reduced. The kit disclosed by the invention is simple to operate, and capable of detecting on a quantitative PCR instrument by only adding a template, so that detection experimental time is greatly shortened. The kit disclosed by the invention is applicable to a single sample and multiple samplers, so that results can be quickly obtained, and therefore, scientific research and clinical detection requirements can be satisfied very well.
Owner:杭州美中疾病基因研究院有限公司
Immunodetection and biochemical detection-based micro-fluidic chip, detector and detection method
PendingCN109030812AFast resultsGet results fastLaboratory glasswaresMaterial analysisMicro fluidicBiochemical detection
The invention provides an immunodetection and biochemical detection-based micro-fluidic chip, a detector and a detection method, wherein the micro-fluidic chip comprises a substrate, wherein a samplefeeding unit, an immunodetection region and a biochemical detection region are arranged on the substrate; the immunodetection region comprises a first reaction unit and a waste liquid recovery unit; afirst curing agent layer is fixed in the first reaction unit; the biochemical detection region comprises a second reaction unit, and a second curing agent layer is fixed in the second reaction unit;the sample feeding unit is communicated with the first reaction unit and the second reaction unit through a micro-channel separately. The detector integrates two different types of detection on the chip, the structure is simple, the flow of the fluid can be accurately controlled, and the detection is fast and accurate.
Owner:DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
Ultra-sensitive magnetic nanometer detection technology for hepatitis C virus (HCV)
InactiveCN106365207AHigh detection sensitivityHigh specificityMicrobiological testing/measurementMicroorganism based processesVirus detectionAntibody
The invention discloses a method for surface functionalization treatment of a Fe3O4 nanoparticle and constructs a novel, simple, cheap, convenient and highly-sensitive HCV virus detection technology through coupling of produced active groups and a HCV antibody. According to the invention, a superparamagnetic polymethyl acrylate microsphere is synthesized by using suspension emulsion polymerization method; the surface of the microsphere is modified with a carboxyl group and coupled with an anti-HCV antibody; and the coupled superparamagnetic polymethyl acrylate microsphere has HCV enrichment efficiency of 94.50%. The invention provides a method for detecting HCV by using a combination of immunomagnetic beads and fluorescent PCR, belonging to the field of health quarantine.
Owner:北京国际旅行卫生保健中心 +1
Transformer winding deformation detection method
InactiveCN109141218ASimple and fast operationGet results fastElectrical/magnetic solid deformation measurementCapacitanceSelf-oscillation
The present invention discloses a transformer winding deformation detection method. The method comprises the steps of: constructing a transformer self-oscillation circuit, wherein the transformer self-oscillation circuit comprises a direct-current high voltage generator, a first control switch, a second control switch, a detected transformer and a detection device; closing the first control switchto allow the direct-current high voltage generator to allow a direct-current high voltage generator to charge a ground equivalent capacitor; disconnecting the first control switch, rapidly closing the second control switch to allow the detected transformer winding to discharge the ground equivalent capacitor, and forming a coupling voltage through magnetic coupling and capacity coupling of the detected transformer winding; detecting the detected transformer through the detection device to obtain the coupling voltage; and finally, according to the coupling voltage, determining whether the detected transformer winding generates deformation or not. The transformer winding deformation detection method can accurately and rapidly determine whether the transformer winding generates deformation or not and the deformation degree.
Owner:YUNNAN POWER GRID CO LTD ELECTRIC POWER RES INST
Multichannel optical fiber fluorescence sensor
The invention provides a multichannel optical fiber fluorescence sensor, which comprises a light source, an excitation light splitting device, a signal light splitting device connected with the excitation light splitting device, a probe array and a light filter device which are connected with the signal light splitting device, an imaging system connected with the light filter device, and a controland data display terminal in communication connection with the light source and the imaging system. The probe array comprises fiber probes. Each fiber probe has an exposed core area and a quantum dot. The excitation light emitted by the light source is coupled into different fiber probes through the light splitting device; evanescent waves generated on the surfaces of the probes excite the quantum dots on the probes to generate fluorescence signals; the signal light of the fluorescence signals is collected by the fiber probes and coupled into the light filter module through the light splitting device, thereby realizing multichannel fluorescence signal acquisition; the detection structure can be visually displayed in the imaging system; and the multichannel optical fiber fluorescence sensor is relatively low in cost, is relatively small in size, is convenient to carry and can quickly obtain the result.
Owner:HUAQIAO UNIVERSITY
Trademark pattern matching method and corresponding device, equipment and medium
PendingCN113569933ARun clearClear logicCharacter and pattern recognitionNeural learning methodsTrademark infringementApproximate matching
The invention discloses a trademark pattern matching method and a corresponding device, equipment and medium, and the method comprises the steps of obtaining the image features of a trademark pattern contained in a to-be-compared picture, constructing a to-be-compared feature matrix, and storing the image features of a single trademark pattern in each row vector; obtaining a preset compared feature matrix of a compared trademark library, wherein each row vector stores an image feature of a single compared trademark in the compared trademark library; calculating the similarity based on the to-be-compared feature matrix and the compared feature matrix to obtain a similar matrix; and screening out elements of which the similarity meets a preset condition from the similar matrix so as to determine suspected trademark patterns having similarity association with the compared trademarks from the trademark patterns, and matching a plurality of first compared trademarks most similar to each suspected trademark pattern for each suspected trademark pattern. According to the invention, operation and judgment of approximate matching of the trademark are realized based on matrix operation, the operation efficiency is high, the response is quick, the overhead is relatively low, and the invention is suitable for identifying suspected trademark infringement information from the product picture.
Owner:GUANGZHOU HUADUO NETWORK TECH
Artificial intelligence system for identifying high myopia retinopathy
PendingCN112053321AEfficiencyIncreased sensitivityImage enhancementImage analysisRetinal lesionImaging processing
The invention relates to the technical field of medical image processing, in particular to an artificial intelligence system for identifying high myopia retinopathy, comprising: an image acquisition module for acquiring an optical coherence tomography image and a fundus reference of a patient to be identified; the first recognition module is used for inputting the optical coherence tomography image into a high myopia retinopathy recognition model, judging whether the optical coherence tomography image has lesion or not and obtaining a lesion type result; the second recognition module is used for inputting the fundus color photograph into a high myopia retinal lesion staging model, judging the retinal lesion staging of the fundus color photograph and obtaining a lesion staging judgment result; and the report generation module is used for generating a diagnosis and treatment suggestion report according to the lesion type result and the lesion staging judgment result. According to the method, the optical coherence tomography image and the fundus color photograph of the patient can be quickly and effectively recognized to recognize common retinopathy of high myopia.
Owner:ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
Method and kit for detecting polymorphism of XRCC1 gene
InactiveCN104593511APurposefulIncrease success rateMicrobiological testing/measurementXRCC1 GeneGenomic DNA
The invention relates to the technical field of molecular biology and particularly relates to a method and kit for detecting the polymorphism of an XRCC1 gene. The method comprises the following steps: (1) by adopting to-be-detected genomic DNA as a template, designing and synthesizing an upstream primer having the sequence as shown in SEQ ID NO: 1 and a downstream primer having the sequence as shown in SEQ ID NO: 2 and carrying out specific PCR amplification; (2) carrying out agarose gel electrophoresis on PCR amplification products and then carrying out gel recovery; and (3) sequencing the gel recovery products and comparing the sequencing results to determine the polymorphism of the gene. The primer pair used in the invention has stronger specificity, after the target gene is subjected to PCR amplification by virtue of the primer pair, the agarose gel electrophoresis is performed to obtain bands with very high teleonomy so that the success rate and reliability of the detection method are improved and the detection of polymorphic sites is of important significance for guiding individual treatment of cancer.
Owner:SHANGHAI PASSION BIOTECHNOLOGY CO LTD
Detection kit and detection method for rapidly detecting novel coronavirus
InactiveCN111337667ASuitable for on-site screeningReduce the risk of infectionMaterial analysis by observing effect on chemical indicatorChemiluminescene/bioluminescenceVirus ProteinHorse radish peroxidase
The invention discloses a detection kit and a detection method for rapidly detecting a novel coronavirus. The kit comprises a group of detection test paper, a positive control, a detection result recording card and an instruction book which are arranged in an aluminum foil bag; the novel coronavirus rapid detection test paper is composed of a bottom plate, a sample area, a reaction area, latex forsealing, a color development layer and a water absorption area; a sample pad and a water absorption pad are arranged on the bottom plate and located on the two opposite sides of the bottom plate respectively, and the sample pad is suction filter paper or a glass fiber membrane and used for bearing an object to be detected; a nitrocellulose membrane is fixed on the bottom plate, and the water absorption pad is water absorption paper; the preparation method comprises the following steps: specifically capturing and combining a coronavirus protein antigen by adopting double enzyme-linked immunosorbent assay through ACE2 and CHOP double proteins; combining a CHOP reaction element in the test paper reaction area with biotin, connecting avidin with horse radish peroxidase, and rapidly a detection result through chemical color development by virtue of a biotin-avidin system, wherein the virus content can be accurately detected by virtue of a microplate reader.
Owner:NANTONG UNIVERSITY
Aquatic pathogenic bacterium colony induction signaling molecule detecting method and special reagent kit thereof
InactiveCN100562742CImprove accuracySimple and fast operationComponent separationMicrobiological testing/measurementHomoserineSignalling molecules
The invention discloses a detection method for quorum sensing signal molecules of aquatic pathogenic bacteria and a special kit thereof. The method includes carrying out the following experiments with the tested bacteria: 1) Streak culture with Agrobacterium tumefaciens A136 on a plate added with X-Gal or ONPG, and observe whether it shows blue; Streak culture on the plate, observe whether it shows purple; 3) Streak inoculate the test bacteria on the LB plate containing 500nM N-acylated homoserine lactone for culture, and then place Violet bacteria CV026 next to the test bacteria streak Stretch in parallel to observe whether the purple lightens or fades; 4) Streak and culture with Esc.coli pSB403 on the plate to observe whether it emits light; in the above experiments, if at least one of the experiments is positive, the tested bacteria produce N - Acylated homoserine lactone. The method of the invention has high accuracy rate, no false negative phenomenon, easy qualitative and quantitative analysis, simple and convenient operation, low cost, quick result, and is suitable for large-scale high-efficiency detection.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode
ActiveCN103173513APromote growthEasy to detectMicrobiological testing/measurementMetaboliteClass II bacteriocin
The invention discloses a method for detecting or detecting in an auxiliary mode whether a peptide to be detected from II type bacteriocin producing strain metabolite contains the II type bacteriocin producing strain quorum sensing signal peptide or is the II type bacteriocin producing strain quorum sensing signal peptide or not. The method comprises the following steps: inoculating the II type bacteriocin producing strain in a liquid improved skimmed milk medium for culturing, adding the peptide to be detected in a liquid improved skimmed milk medium at any time before arriving a stabilization stage, continuously culturing for 10-16 hours to obtain the culture; and detecting whether the II type bacteriocin is existed in the culture or not; if the II type bacteriocin is existed in the culture, the peptide to be detected contains or contains as a candidate, or the candidate is the quorum sensing signal peptide of the II type bacteriocin producing strain. The invention selects the improved skimmed milk medium to discriminate the quorum sensing autoinducing peptide, compared with the skimmed milk medium, the speed is more rapid, and the detection time is greatly shortened.
Owner:苏州益优生物科技有限公司
Detection method for obligate hydrocarbon oxidizing bacteria
ActiveCN102453746BEasy to operateShort training periodMicrobiological testing/measurementColor changesShort terms
The invention discloses a detection method for obligate hydrocarbon oxidizing bacteria, belonging to the technical field of microorganism detection methods. The detection method comprises the following steps of: suspending and diluting a soil sample with a basic culture medium; performing short-term culturing in combination with a selective culture carbon source and an oxidation reduction indicating dye for 3-5 days; and computing the quantity of obligate hydrocarbon oxidizing bacteria in the soil sample according to the color change of an airtight culture tube through a computer program. A detection technology for obligate hydrocarbon oxidizing bacteria disclosed by the invention has the advantages of simple operating process, economic efficiency, practicability, quickly-obtained result in a short period of time, high specificity, capability of effectively shielding the interference of other bacteria on counting and simple and accurate counting process, and is suitable for large-scale industrial exploration measurement with a large sample quantity.
Owner:CHINA PETROLEUM & CHEM CORP +1
Data processing method, device and system
ActiveCN110084455AChange life cycleLife cycle design or changeResourcesSurvival functionComputer science
The invention discloses a data processing method, device and system. The data processing method comprises the steps of determining an absolute survival function curve of a target client based on a client characteristic of the target client, a characteristic coefficient of the client characteristic and a reference risk rate; matching the absolute survival function curve of the target client with the target survival curve of the target client by adjusting the client characteristics of the target client; and determining one or more feature schemes corresponding to the target survival curve of thetarget client according to an adjustment result of the client feature of the target client. The client life cycle can be automatically and effectively managed.
Owner:ALIBABA GRP HLDG LTD
Method for determining motion interference of plugging mechanism
ActiveCN112747633AThe result is accurateSimple resultGeometric CADRocket launchersRotational axisClassical mechanics
The invention relates to a method for determining motion interference of a plugging mechanism. The method comprises the following steps: (1) simplifying an actual model of the plugging mechanism into a physical model; (2) carrying out stress and motion analysis on the simplified physical model; (3) using the plugging mechanism as a motion system, wherein the whole system is in compound motion, absolute motion is fixed-axis rotation of a rotating arm of the plugging mechanism around a rotating shaft of the plugging mechanism, relative motion is movement of a plug in a long circular hole, and transport motion is fixed-axis rotation of the plug around a plug pin; and (4) obtaining a position map of any angle in the motion process of the plugging mechanism according to a motion analysis result, and judging whether interference exists in the motion process of the plugging mechanism according to the position map. According to the method, a kinematics simulation model does not need to be established, and an actual verification test does not need to be carried out; and the position corresponding to any angle in the rotating process can be theoretically obtained and displayed through drawing, then whether interference exists in the moving process of the plugging mechanism or not is judged, and the obtained result is real, accurate and visual.
Owner:CAIHONG DRONE TECH CO LTD
Quality evaluation method of traditional Chinese medicine-fructus evodiae
InactiveCN107356717AGet results fastImprove accuracyPreparing sample for investigationTesting medicinal preparationsMedicineBULK ACTIVE INGREDIENT
The invention provides a quality evaluation method of traditional Chinese medicine-fructus evodiae. The method comprises the following steps: different fructus evodiae samples are ground and screened, and fructus evodiae powder is obtained; the fructus evodiae powder is weighed, ground repeatedly with a chloral hydrate solution and transferred into volumetric flasks, glycerin is added, constant volume is reached with the chloral hydrate solution, and suspensions are obtained; the suspensions are drawn for slice production, and nonglandular hairs are observed under a microscope and counted; the average number of the nonglandular hairs in the fructus evodiae samples per milligram is counted, and the product with the average number larger than or equal to 543.83 per milligram is qualified. With adoption of the method, quality of the traditional Chinese medicine-fructus evodiae is judged by determining the micro-characteristic constant value of the non-glandular hairs in the different fructus evodiae samples, and one novel method for evaluating quality of fructus evodiae is provided. Compared with a method for identifying quality of fructus evodiae with a main active ingredient determining process, the method has the advantages of being simple and convenient and obtaining results more quickly; compared with a conventional method for identifying fructus evodiae on the basis of morphology microscopic characteristics, the quality evaluation method is high in accuracy.
Owner:LINYI UNIVERSITY
Reciprocating mechanical equipment fault detection method based on real-time vibration data
ActiveCN112665713AImprove engineering applicabilitySmall amount of calculationMachine part testingInternal-combustion engine testingData segmentControl engineering
The invention discloses a reciprocating mechanical equipment fault detection method based on vibration data, and relates to the technical field of diesel engine operation state monitoring. The method comprises the following steps: firstly, aiming at target equipment, optimizing a measuring point channel which is most sensitive to an operation state through working condition sensitivity analysis; in the actual operation process of the target equipment, collecting a vibration signal of the optimal measuring point channel in real time; performing Fourier transform on each new data segment to obtain a Fourier coefficient vector; continuously arranging the Fourier coefficient vectors of different data segments in the time direction to obtain 'time-frequency-amplitude' three-dimensional joint distribution of the vibration data of the channel, and constructing 'time-frequency-amplitude increment' three-dimensional joint distribution and enhanced 'time-frequency-amplitude increment' three-dimensional joint distribution; and finally, comparing with operation condition data, and detecting fault characteristics. According to the invention, information extraction can be effectively carried out on reciprocating mechanical equipment in real time, and faults can be found and positioned in time.
Owner:中国人民解放军92942部队
A Method for Detecting Soil Pollution Toxicity by Electrical Signal of Dual-chamber Microbial Fuel Cell
InactiveCN103645231BLow costLow running costMaterial analysis by electric/magnetic meansPotassium ferricyanidePollution
The invention discloses a method for detecting the pollution toxicity of soil by adopting electric signals of a double-chamber microbial fuel cell and belongs to the technical field of detection of soil pollution. According to the method, electric quantity generated by decomposing organic matters through soil microorganisms is used as an index for detecting the pollution toxicity of the soil; compared with a traditional method for detecting the toxicity by microorganism activity, biomass and the like, the method has the advantages of low cost, easiness in operation and short detection period. The double-chamber microbial fuel cell used in the method comprises an anode, a cathode, an anode fixing groove, a cathode fixing groove, an anode chamber, a cathode chamber, a top cover of the anode chamber, a top cover of the cathode chamber, a diaphragm, a rubber cushion, a lead wire, a load, bolts and nuts. Before the double-chamber microbial fuel cell is operated, polluted soil and glucose are mixed and are added into the anode chamber; a potassium ferricyanide solution is introduced into the cathode chamber to be used as an electron acceptor; after the double-chamber microbial fuel cell is operated for 48 hours, the generated electric quantity in the 48 hours is calculated and is used for evaluating the toxicity of pollutants. The method for detecting the pollution toxicity of the soil by adopting the electric signals of the double-chamber microbial fuel cell has high scientific values and application values.
Owner:NANJING NORMAL UNIVERSITY
Intelligent police car mobile big data platform
InactiveCN110598023ASave storage spaceSmall amount of calculationDigital data information retrievalCharacter and pattern recognitionComputational modelFace model
The invention discloses an intelligent police car mobile big data platform. Face data is processed by a face integration model and then is stored in a face model library. License plate data is processed by a license plate integration model and then stored in a license plate model library. The face model library and the license plate model library are directly installed on a police car through a server device and a storage device. During data processing, after facial photos are automatically collected through portrait collection equipment, photos are input into a data calculation unit, query and comparison are performed by utilizing the data calculation unit, calculation results are obtained after calculation, and then result output is performed. According to the invention, a mode of establishing a data model is used to pre-process various data and compress and store the data in the smart police car, so that the storage space is greatly saved and the calculation amount is reduced; through big data integration and compression technologies, data is subjected to deduplication and modeled storage in a data storage node of a police car, and a calculation unit can quickly obtain a resultonly by calculating model node data.
Owner:HANTENG AUTOMOBILE CO LTD
Data selection method for diagnostic predictions
PendingCN113611380ALow implementation costAvoid cumbersome stepsCharacter and pattern recognitionNatural language data processingMedical recordData ingestion
The invention relates to the technical field of data message distribution, in particular to a data selection method for diagnosis prediction. The method comprises the following steps: S1, analyzing an electronic medical record and obtaining text data; S2, extracting the medical record data and diagnosis data; S3, uploading the data to a medical record library, and searching for related data; S4, comparing the medical record data and the diagnosis data; S5, performing data similarity arrangement; S6, obtaining multiple sets of related diagnosis schemes, wherein the text data refer to complete diagnosis medical record data information of a patient in a special department. According to the method, the tedious steps of medical record modeling and machine learning are avoided, the implementation cost of a diagnosis prediction scheme is reduced, multiple diagnosis schemes are provided when doctors diagnose special medical records or medical records containing complications, the situation that the complex medical records are missed or mistakenly diagnosed is avoided, and meanwhile, the searched comparison medical records provide treatment methods and treatment effects of previous medical records for doctors, so that the doctors can conveniently treat patients after diagnosis and prediction.
Owner:喀什地区第一人民医院
Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode
ActiveCN103173513BPromote growthEasy to detectMicrobiological testing/measurementMetaboliteClass II bacteriocin
The invention discloses a method for detecting or detecting in an auxiliary mode whether a peptide to be detected from II type bacteriocin producing strain metabolite contains the II type bacteriocin producing strain quorum sensing signal peptide or is the II type bacteriocin producing strain quorum sensing signal peptide or not. The method comprises the following steps: inoculating the II type bacteriocin producing strain in a liquid improved skimmed milk medium for culturing, adding the peptide to be detected in a liquid improved skimmed milk medium at any time before arriving a stabilization stage, continuously culturing for 10-16 hours to obtain the culture; and detecting whether the II type bacteriocin is existed in the culture or not; if the II type bacteriocin is existed in the culture, the peptide to be detected contains or contains as a candidate, or the candidate is the quorum sensing signal peptide of the II type bacteriocin producing strain. The invention selects the improved skimmed milk medium to discriminate the quorum sensing autoinducing peptide, compared with the skimmed milk medium, the speed is more rapid, and the detection time is greatly shortened.
Owner:苏州益优生物科技有限公司
Electromagnetic safety analysis method for near field of plane phased control array antenna
ActiveCN102539940BHigh precisionGuaranteed accuracyAntenna radiation diagramsArray elementPhase control
The invention provides an electromagnetic safety analysis method for a near field of a plane phased control array antenna on a system platform, which analyses electromagnetic safety of personnel and equipment close to the plane phased control array antenna on the system platform by calculating the near field electromagnetic environment of phase control array antenna radiation. The near field of the plane phased control array antenna is obtained by overlaying a radiation field of each active (motivation) array element in a array; enabling a near field area of the plane array antenna to be a far field area relative to the array elements; obtaining the near field of the array antenna after overlaying and rectifying the radiation far fields and solving the radiation far field of each array element in the array; and diagnosing the electromagnetic safety of the personnel and the equipment which are located at the near field of the phase control array antenna on the system platform by comparing near field electric field data with an electric field limit value specified by a electromagnetic safety standard. Each step of the method is based on strict principles, and the strictness of the principles ensures accuracy of calculation results, so that the electromagnetic safety of the personnel and the equipment located in the antenna near field area can be accurately analyzed.
Owner:CHINA SHIP DEV & DESIGN CENT
Q-PCR detection method for colorectal cancer marker DCD
PendingCN112831565AAid in early diagnosisSimple procedureMicrobiological testing/measurementDNA/RNA fragmentationRNA extractionColon rectal cancer
The invention relates to the technical field of in-vitro detection, in particular to a Q-PCR detection method of a colorectal cancer marker DCD. The method comprises the steps of RNA extraction of a sample, reverse transcription and Q-PCR amplification. The DCD is used as a colorectal cancer candidate diagnosis index and treatment target, RNA of cells or tissues is firstly extracted, an internal reference primer and a DCD gene primer are set to perform reverse transcription on the RNA to obtain two groups of different amplification products, Q-PCR reaction is performed respectively to obtain melting curves and amplification curves of the two groups of amplification products respectively, and the expression level of the DCD gene is calculated; then, theexpression level of the DCD geneis compared with the mRNA concentration of the DCD in the normal tissue or cell, and if the mRNA concentration of the DCD in the tested tissue or cell is more than one time higher than that of the normal tissue or cell, the tested tissue or cell can be considered to be the colorectal cancer tissue or colorectal cancer cell, so that early diagnosis of the colorectal cancer is facilitated.
Owner:广州医科大学附属中医医院
Method for rapidly acquiring size and distribution of ultra-fast demagnetization of material
InactiveCN102809733BLow costEasy to implementMagnetic property measurementsMaterial analysis using radiation diffractionEnergy windowDensity functional theory
The invention relates to a material analyzing and testing method, and particularly relates to a method for rapidly acquiring size and distribution of ultra-fast demagnetization of a material. The method specifically comprises the following steps: measuring and researching crystallography parameters of the material by using an X-ray diffracmeter, wherein the crystallography parameters include the spatial symmetric group to which the material belongs, a lattice constant and a fractional coordinate to which each atom corresponds; acquiring the ground state property of the material by applying a density functional theory in combination with the crystallography parameters of the material, wherein the ground state property mainly comprises a magnetic moment, a Fermi surface and an energy band structure of the material; determining spin density matrixes and electric dipole transition matrixes on a k point inside a reduced brillouin zone of the researched material; calculating a spin expected value difference matrix, a dipole transition matrix, and an energy window selecting matrix; researching the size of the demagnetization of the material; and acquiring the distribution situation of a femtosecond magnetism in a whole momentum space through symmetry operation. The method has the characteristics of being small in desired resource, low in cost, and simple and feasible.
Owner:LANZHOU UNIVERSITY
A Digital Image Analysis Method for Obtaining Object Deformation
ActiveCN104200474BSimple wayNot affected by test environmentImage analysisPattern recognitionFast Fourier transform
The invention discloses a digital image analysis method for obtaining object deformation quantity. The digital image analysis method for obtaining the object deformation quantity includes steps: S1, obtaining object images before and after deformation; S2, respectively selecting analysis areas of the same coordinate from the same portion of the same object in two images; S3, respectively performing fast Fourier transformation and mathematical treatment on gray values of pixel dots in the two analysis areas so as to obtain functions related to the gray values and the deformation quantity of the pixel dots in the two analysis areas and related to a variable in the mathematical treatment; S4, respectively calculating four different functions obtained when four different fixed values are respectively assigned to the variable in the mathematical treatment; S5, performing the mathematical treatment on the four different functions obtained in the step S4 so as to obtain a fifth function; S6, performing the Fourier transformation on the fifth function so as to obtain an impulse function including the object deformation quantity, and performing calculation so as to obtain the position of the highest impulse point in the impulse function, wherein the coordinate of the position of the highest impulse point in the impulse function is the deformation quantity caused by object deformation in the X axis direction and the Y axis direction.
Owner:HUAZHONG UNIV OF SCI & TECH
Horn temperature testing system and method based on phase change measurement
Owner:SHANGHAI FOURSEMI SEMICON CO LTD
Cleaning agent separating device used for cleaning of inner wall of copper pipe
PendingCN108079632AImprove reliabilityImprove versatilityHollow article cleaningStationary filtering element filtersEngineeringCleansing Agents
The invention discloses a cleaning agent separating device used for cleaning of the inner wall of a copper pipe. The device comprises a collecting barrel, and the collecting barrel communicates with the output end of the copper pipe through a cleaning pipe; a separating barrel is installed between the cleaning pipe and the collecting barrel, the separating barrel is composed of an observation barrel and a filter barrel, the observation barrel and the filter barrel are separately a round barrel structure, the observation barrel is fixed at the upper end of the filter barrel, and the lower end of the filter barrel communicates with the collecting barrel; the output end of the cleaning pipe is in seal connection with the observation barrel, a protective cover is installed on the top of the observation barrel, and one side of the protective cover is rotatably connected with the observation barrel; and a filter net is installed in the filter barrel, the filter net and the inner wall of thefilter barrel are rotatably connected, a turnover mechanism used for turning over the filter net is installed on the outer side of the filter barrel, and the turnover mechanism extends into the filterbarrel and is fixedly connected with the filter net. The device provided by the invention can help a worker to intuitively observe the state of blown sponge balls, and passing is safe and reliable.
Owner:CHONGQING LONGYU PRECISION COPPER TUBE CO LTD
Rapid detection method for nitrification effect of nitrobacteria
InactiveCN106916873AEasy to operateGet results fastMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementNitrite nitrogenEnvironmental chemistry
The invention discloses a rapid detection method for nitrification effect of nitrobacteria. The method comprises the following steps: 1) preparing a nitrite liquid, which comprises preparation of nitrite nitrogen NO2-N standard operating fluid or taking of nitrite-containing aquatic product culture water; 2) preparing nitrated reaction systems with different volume gradients; 3) performing the nitrification for 16-22 hours in a thermotank at the temperature of 25-35 DEG C; 4) preparing a colorimetric reagent; 5) performing colorimetric detection; and 6) performing assessment on the colorimetric result and the nitrification effect. The method is simple, visual and practical, the obtained result is rapid; direct and rapid detection is carried out on the nitrification effect of nitrobacteria by a culture filed, whether the nitrification effect is generated on the nitrobacteria or not can be qualitative, the level of the quantification nitrification effect is carried out, and the method has guidance meaning for monitoring aquatic product culture water quality and solving the problem of the aquatic product culture.
Owner:无锡市中渔健宝生物有限公司 +1
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