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Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode

A quorum sensing signal and auxiliary detection technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of long cycle and slow growth, and achieve low cost, high accuracy, and easy qualitative and quantitative analysis. Effect

Active Publication Date: 2014-12-10
苏州益优生物科技有限公司
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Problems solved by technology

[0004] Skim milk medium is suitable for the growth of most lactic acid bacteria, but many lactic acid bacteria grow slowly in skim milk medium, so it takes a long time to detect self-inducing peptides with this medium

Method used

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  • Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode
  • Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode
  • Method for detecting or detecting II type bacteriocin producing strain quorum sensing signal peptide in auxiliary mode

Examples

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Embodiment 1

[0032] Example 1. Detection or auxiliary detection of whether the peptide to be detected derived from the class II bacteriocin-producing bacteria L.pentosus 31-1 is a quorum sensing signal peptide

[0033] Lactobacillus pentosus 31-1 is a class II bacteriocin producer (Guorong Liu, Yanni Lv, Pinglan Li, Kang Zhou, Jinglan Zhang. Pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31- 1 isolated from Xuan-Wei Ham, a traditional China fermented meat product. Food Control, 2008, 19: 353-359. Zhang Jinlan, Liu Guorong, Shang Nan, Cheng Wanpeng, Chen Shangwu, Li Pinglan . Purification and Partial Amino Acid Sequence of Pentocin 31-1, an Anti-Listeria Bacteriocin Produced by Lactobacillus pentosus 31-1.Journal of Food Protection, Volume 72, Number 12.2524-2529.), the presence of Class II bacteriocin production was identified Quorum sensing regulation phenomenon (see the end of this example for specific identification methods). This example will describe i...

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Abstract

The invention discloses a method for detecting or detecting in an auxiliary mode whether a peptide to be detected from II type bacteriocin producing strain metabolite contains the II type bacteriocin producing strain quorum sensing signal peptide or is the II type bacteriocin producing strain quorum sensing signal peptide or not. The method comprises the following steps: inoculating the II type bacteriocin producing strain in a liquid improved skimmed milk medium for culturing, adding the peptide to be detected in a liquid improved skimmed milk medium at any time before arriving a stabilization stage, continuously culturing for 10-16 hours to obtain the culture; and detecting whether the II type bacteriocin is existed in the culture or not; if the II type bacteriocin is existed in the culture, the peptide to be detected contains or contains as a candidate, or the candidate is the quorum sensing signal peptide of the II type bacteriocin producing strain. The invention selects the improved skimmed milk medium to discriminate the quorum sensing autoinducing peptide, compared with the skimmed milk medium, the speed is more rapid, and the detection time is greatly shortened.

Description

technical field [0001] The invention relates to a method for detecting or assisting in detecting whether the peptide to be detected derived from the metabolites of class II bacteriocin producing bacteria contains the quorum sensing signal peptide of class II bacteriocin producing bacteria or whether it is a quorum sensing signal peptide of class II bacteriocin producing bacteria . Background technique [0002] During the growth process, microorganisms can spontaneously produce and release some specific signal molecules, and can sense the changes in their concentration and regulate their group behavior. This phenomenon is called quorum sensing (QS), and the signal molecules produced are called self-induction. Things (autoinducer, AI). It is known that many species of bacteria can regulate physiological behavior through quorum sensing. At present, there have been many research reports and related patents on the detection of some G-bacterial quorum sensing signal molecules - ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 李平兰张香美张宝刘丽莎
Owner 苏州益优生物科技有限公司
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