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Immunodetection and biochemical detection-based micro-fluidic chip, detector and detection method

A microfluidic chip and biochemical detection technology, applied in chemical instruments and methods, instruments, measuring devices, etc., can solve the problems of precise control of unfavorable fluids, and achieve the effect of precise control of flow and simple operation

Pending Publication Date: 2018-12-18
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sample port, reagent port and detection tank are directly connected without precise control of the fluid.

Method used

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  • Immunodetection and biochemical detection-based micro-fluidic chip, detector and detection method
  • Immunodetection and biochemical detection-based micro-fluidic chip, detector and detection method
  • Immunodetection and biochemical detection-based micro-fluidic chip, detector and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] A detector based on immune detection and biochemical detection, including a microfluidic chip, the microfluidic chip includes a substrate, and a sampling unit, an immunological detection area, and a biochemical detection area are arranged on the substrate;

[0114] The sampling unit includes a sampling tank;

[0115] The immune detection area includes a first reaction unit and a waste liquid recovery unit, and the first reaction unit and the waste liquid recovery unit are communicated through a microchannel provided with a third valve; the first reaction unit includes a first reaction tank, and the first reaction unit is fixed There is a first curing agent layer; the waste liquid recovery unit includes a waste liquid tank;

[0116] The biochemical detection area includes a second reaction unit, the second reaction unit includes a second reaction tank, and a second curing agent layer is fixed in the second reaction unit;

[0117] The sampling unit communicates with the ...

Embodiment 2

[0129] A detector based on immunodetection and biochemical detection includes a microfluidic chip, the microfluidic chip includes a substrate, and the substrate is provided with a sampling unit, an immunological detection area, and a biochemical detection area;

[0130] The sampling unit includes a sampling tank, a first quantitative tank, a collection tank and a first distribution tank, and the sampling tank, the first quantitative tank and the collection tank are connected through microchannels in sequence, and the first quantitative tank is also connected with the first distribution tank. The grooves are connected; the first valve is set on the microchannel where the first quantitative groove communicates with the first distribution groove;

[0131] The immune detection area includes a second quantitative tank, a second distribution unit, at least one first reaction unit, and a waste liquid recovery unit connected in sequence; it also includes a first liquid storage unit, an...

Embodiment 3

[0154] A detection instrument based on immunoassay and biochemical detection (such as figure 1 shown), including microfluidic chips (such as figure 2 As shown), the microfluidic chip includes a substrate, and the substrate is provided with a sampling unit, an immune detection area and a biochemical detection area;

[0155] The sampling unit includes a sampling tank 1, a first quantitative tank 2, a collection tank 3 and a first distribution tank, and the sampling tank 1, the first quantitative tank 2 and the collection tank 3 are connected through microchannels successively, and the first quantitative The groove 2 is also communicated with the first distributing groove; the first valve 4 is arranged on the microchannel that the first quantitative groove 2 communicates with the first distributing groove;

[0156] The immune detection area includes a second quantitative tank 10, a second distribution unit, at least one first reaction unit, and a waste liquid recovery unit conn...

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PUM

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Abstract

The invention provides an immunodetection and biochemical detection-based micro-fluidic chip, a detector and a detection method, wherein the micro-fluidic chip comprises a substrate, wherein a samplefeeding unit, an immunodetection region and a biochemical detection region are arranged on the substrate; the immunodetection region comprises a first reaction unit and a waste liquid recovery unit; afirst curing agent layer is fixed in the first reaction unit; the biochemical detection region comprises a second reaction unit, and a second curing agent layer is fixed in the second reaction unit;the sample feeding unit is communicated with the first reaction unit and the second reaction unit through a micro-channel separately. The detector integrates two different types of detection on the chip, the structure is simple, the flow of the fluid can be accurately controlled, and the detection is fast and accurate.

Description

technical field [0001] The invention belongs to the technical field of detection equipment and methods, and relates to a microfluidic chip, a detector and a detection method based on immune detection and biochemical detection. Background technique [0002] The judgment of disease needs to combine different information combinations, such as biochemical detection, immune detection and imaging detection. At present, most of the detection equipment is oriented to a certain type of detection, such as biochemical analyzers and immune analyzers, all of which use a certain type of substance as the detection substance. In addition, the existing testing equipment is large in size and expensive, and it is generally difficult for small grassroots hospitals to afford it. [0003] Due to its high sensitivity and high specificity, chemiluminescent immunoassay has basically replaced radioimmunoassay and enzyme-linked immunoassay, and has become the most widely used immunoassay in the field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543B01L3/00
CPCG01N33/531G01N33/54326B01L3/5027
Inventor 刘仁源焦政李重阳宋建军顾志鹏李建霖
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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