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Quality evaluation method of traditional Chinese medicine-fructus evodiae

A technology of quality evaluation and Evodia rutaecarpa, which is applied in the field of quality evaluation of traditional Chinese medicine Evodia rutaecarpa, can solve the problems of large differences in microscopic identification and cumbersome identification operations, and achieve the effect of high accuracy and simple and convenient method

Inactive Publication Date: 2017-11-17
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of cumbersome identification of effective components of Evodia rutaecarpa and large differences in conventional microscopic identification, the present invention provides a quality evaluation method for traditional Chinese medicine Evodia rutaecarpa, which has countable microscopic features, high accuracy, and strong operability

Method used

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  • Quality evaluation method of traditional Chinese medicine-fructus evodiae
  • Quality evaluation method of traditional Chinese medicine-fructus evodiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Quality Evaluation of Evodia Evodia

[0030] Eight samples of decoction pieces of Evodia rutaecarpa were purchased, and the places of origin are shown in Table 1.

[0031] Table 1 Origin of decoction pieces of Evodia rutaecarpa

[0032] serial number

product name

Specification

place of origin

1

Evodia

Pieces

Guizhou

2

Evodia

Pieces

Jiangxi

3

Evodia

Pieces

Jiangxi

4

Evodia

Pieces

Jiangxi

5

Evodia

Pieces

zhejiang

6

Evodia

Pieces

zhejiang

7

Evodia

Pieces

Jiangxi

8

Evodia

Pieces

zhejiang

9

Evodia

Pieces

zhejiang

[0033] The quality evaluation is carried out according to the following method steps:

[0034] (1) Pulverize different Evodia rutaecarpa samples through a 100-mesh sieve to obtain Evodia rutaecarpa powder;

[0035] (2) Accurately weigh 200 mg of each Evodia rutaecarpa...

Embodiment 2

[0046] Example 2 The quality of Evodia rutaecarpa was evaluated by limonin content.

[0047] The content of limonin in Evodia rutaecarpa was determined by high performance liquid chromatography.

[0048] 2.1 Chromatographic conditions

[0049] Chromatographic column: Inertsil ODS-3 (4.6×250mm, 5μm); mobile phase: [acetonitrile-tetrahydrofuran (25:15)]-0.02% phosphoric acid solution (35:65); detection wavelength: 215 nm; flow rate: 1 mL / min; column temperature: 30°C.

[0050] 2.2 Preparation of reference solution

[0051] Take an appropriate amount of limonin reference substance, weigh it accurately, add methanol to make a mixed solution containing 0.104 mg of limonin per 1 mL, and obtain it.

[0052] 2.3 Preparation of the test solution

[0053] Take about 0.3 g of Evodia rutaecarpa sample powder (through the No. 3 sieve), accurately weigh it, put it into a stoppered Erlenmeyer flask, accurately add 25 mL of 70% ethanol, weigh it, soak for 1 hour, and perform ultrasonic t...

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Abstract

The invention provides a quality evaluation method of traditional Chinese medicine-fructus evodiae. The method comprises the following steps: different fructus evodiae samples are ground and screened, and fructus evodiae powder is obtained; the fructus evodiae powder is weighed, ground repeatedly with a chloral hydrate solution and transferred into volumetric flasks, glycerin is added, constant volume is reached with the chloral hydrate solution, and suspensions are obtained; the suspensions are drawn for slice production, and nonglandular hairs are observed under a microscope and counted; the average number of the nonglandular hairs in the fructus evodiae samples per milligram is counted, and the product with the average number larger than or equal to 543.83 per milligram is qualified. With adoption of the method, quality of the traditional Chinese medicine-fructus evodiae is judged by determining the micro-characteristic constant value of the non-glandular hairs in the different fructus evodiae samples, and one novel method for evaluating quality of fructus evodiae is provided. Compared with a method for identifying quality of fructus evodiae with a main active ingredient determining process, the method has the advantages of being simple and convenient and obtaining results more quickly; compared with a conventional method for identifying fructus evodiae on the basis of morphology microscopic characteristics, the quality evaluation method is high in accuracy.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and relates to a quality evaluation method of traditional Chinese medicine Evodia rutaecarpa. Background technique [0002] The quality standard of traditional Chinese medicine is an important part of the national drug standard. The composition of traditional Chinese medicine is complex and there are many varieties. The current quality standards for drug quality control are limited to the qualitative identification of most medicinal materials and the quantitative detection of a single chemical component of a small number of medicinal materials. The measurement and control of any component in traditional Chinese medicine products cannot represent it. The overall quality, controllability and specificity are not yet perfect. It is imprecise and perfect to use chemical drug quality control methods as a model for traditional Chinese medicine products. The quality control of traditional Chi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15G01N1/28
CPCG01N33/15G01N1/28
Inventor 陈聪慧
Owner LINYI UNIVERSITY
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