50 results about "Hydrated chloral" patented technology

The invention discloses an imaging model in small animal living bodies with echinococcus granulosus and a construction method thereof. The construction method comprises the steps of: feeding CF-1 male mice; carrying out in vitro culture and drug treatment on protoscolex; carrying out in vitro fluorescence imaging and fluorescence gradient analysis; pretreating mice with depilatory paste for experimental animals, injecting the abdominal cavity of each mouse with chloral hydrate anesthetic, implanting three groups of stained scoleces under Glisson capsules by injection, dividing the mice into three groups according to different groups of injected protoscolex; in a control group, injecting the livers of mice with protoscolex on which drug treatment is not carried out, collecting images every 12 hours after injection, carrying out fluorescence intensity analysis on the ROIs region, putting into a polyethylene cage after imaging, and carrying out in vitro fluorescence gradient detection in the positive control group. The construction method constructs a mouse model with echinococcus granulosus which can express luciferase, provides a platform for drug sensitive tests in living bodies with echinococcosis, and can monitor the growth and transfer of hydatid in the living model dynamically in a long term.

The invention discloses a preparation method for wheat leaf stomata guard cell observation samples, and relates to the plant research technology. The method comprises the steps of preparation of a material to be treated, fixation of the material to be treated, dehydration and whole transparency. The method is characterized in that a transparent reagent adopted by the whole transparency is obtained by mixing chloral hydrate, distilled water and glycerol according to the weight ratio of 4 g: 1 ml: (0-0.5) ml (the ratio can be rationally widened to a range). For preparing the wheat leaf stomata guard cell observation samples, clear microscopic observation images are obtained.

The invention relates to a preparation method for phenazine-1-carboxylic acid. The preparation method comprises the following steps: reacting aniline with chloral hydrate and hydroxylamine to produce alpha-oximidoacetanilide, treaing alpha-oximidoacetanilide with concentrated sulfuric acid to obtain isatin, reacting isatin with hydrogen peroxide so as to obtain 2-amino-3-nitrobenzoic acid and then carrying out Sandmeyer reaction to prepare 2-bromo-3-nitrobenzoic acid; and subjecting prepared 2-bromo-3-nitrobenzoic acid and aniline to Jourdan-Ullmann reaction so as to obtain substituted diphenylamine and carrying out ring closure to prepare phenazine-1-carboxylic acid. Compared with the prior art, the preparation method provided by the invention has the advantages of easy availability of raw materials, easy control of reaction, mild reaction conditions, easy post-treatment, high overall yield, as high as 32 to 47%, and suitability for industrial production; and compared with conventional method for production of shenqinmycin from ferment powder, the method provided by the invention enables cost to be greatly reduced.

Disclosed is a tower type continuous dewatering technology of chloral hydrate, technical characteristic of which is that dilute sulphuric acid is discharged out from the bottom of the tower, the chloral hydrate enters from a certain portion at the bottom of the tower, concentrated sulfuric acid enters from the top of the tower, and refined chloral is discharged out from the top of the tower. The whole process is the continuous dewatering operation and the technique is simple, fast and fluently, which realizes continuous dewatering of chloral hydrate with relatively less equipments and relatively low energy consumption. The technique can be controlled easily and operated relatively stably. Compared with other existing dewatering techniques, the invention not only can reduce investment of equipments, but also is small in area and low in energy consumption. Further, the invention can decrease dewatering cost of chloral hydrate obviously and improve product quality.

The invention belongs to the field of biochemistry and pharmacy, and discloses application of chloral hydrate in preparing a metabolism regulator. The metabonomics is adopted to research the influenceof the chloral hydrate on metabolism, and the application of the chloral hydrate in preparing the metabolism regulator is provided and has a better clinic application value.

The invention discloses a method for staining the iron element in plants. The method comprises steps of: cutting a plant material into small pieces; soaking the small pieces into a Perls staining fluid to be stained; rinsing the stained small plant material pieces with deionized water; putting the rinsed small plant material pieces into a chloral hydrate test solution until the small plant material pieces are completely transparent; and taking out the small plant material pieces so as to obtain the materials with stained iron element. According to the method, a Prussian blue Perls iron staining method and a chloral hydrate transparency method are organically combined together, the internal iron distribution of the plant can be integrally displayed under the condition of keeping the plant material complete to the greatest extent; the method has a short period, and a result is generally obtained after a week through adopting a slice method, but can be obtained in a day through utilizing the method; and the method has the advantages of simplicity in operation and no adoption of expensive equipment.

The invention discloses a synthesis technology of a cardiovascular medicine Apabetalone, and relates to the technical field of organic synthesis of medicines. The synthesis technology comprises the following steps: with 3,5-dimethoxyaniline as a raw material, firstly performing a condensation reaction on the 3,5-dimethoxyaniline, hydroxylamine sulfate and chloral hydrate to obtain N-(2,4-dimethoxyphenyl)-2-(oximido)acetamide, then performing a cyclization reaction to obtain 5,6-dimethoxy isatin under the effect of concentrated sulfuric acid, then performing an oxidation reaction on the 5,6-dimethoxy isatin and ammonia water or ammonia gas to obtain 2-amino-4,6-dimethoxybenzonitrile in the presence of divalent copper and an oxidant, finally performing a condensation reaction on the 2-amino-4,6-dimethoxybenzonitrile and 4-(2-hydroxyethoxy)-3,5-dimethylbenzaldehyde in an organic solvent under the effect of an alkaline substance to obtain the Apabetalone. Compared with the synthesis technology recorded in the existing literature, the synthesis technology provided by the invention has the advantages as follows: the raw material is cheap and easy to obtain, a reaction condition is mild,high-temperature and high-pressure conditions are not required, the posttreatment operation is simple, a synthesis route is short, and industrial production is easy.

An industrialization preparation method for 4-fluoroisatin is disclosed by the invention, and comprises: (a) dissolving chloral hydrate and sodium sulfate in water, heating to 30 DEG C-35 DEG C, then successively adding m-fluoroaniline, concentrated hydrochloric acid and an aqueous solution of hydroxylamine hydrochloride, heating to 80 DEG C-90 DEG C for continually reaction, after a complete reaction, air-pump filtering and washing the filter cake with water, and obtaining the intermediate; and (b) heating concentrated sulfuric acid to 50 DEG C-60 DEG C, successively adding TEBBE reagent and the intermediate obtained in step (a), then continually heating to 80 DEG C-85 DEG C and reacting, after a complete reaction, cooling and precipitating an orange red precipitate, and therefore obtaining the 4-fluoroisatin product. The invention also discloses the corresponding 4-fluoroisatin product. The industrialization preparation method can help to obtain the product with a simple process, a high yield and low cost, and is convenient to control quality and applicable in industrialization production in a large scale.

The invention discloses a method for establishing a Mongolian gerbil orthotopic liver transplantation model and a method for separating hepatic stellate cells. The method for establishing the Mongolian gerbil orthotopic liver transplantation model comprises the steps that a donor is narcotized, and a grafted liver is cut and taken out of the donor; a receptor is narcotized, the liver is cut out, the grafted liver is implanted into the receptor with the liver cut out; the survival rate of the Mongolian gerbil orthotopic liver transplantation model is calculated. Mongolian gerbil orthotopic liver transplantation is executed, changes of the Mongolian gerbil orthotopic liver transplantation model are observed, and the survival rate is calculated. The method for separating the hepatic stellate cells of Mongolian gerbil comprises the steps that chloral hydrate is used for intraperitoneal injection to narcotize the male Mongolian gerbil, or the male Mongolian gerbil which dead just now is selected, and the grafted liver is cut and taken out of the donor; the hepatic stellate cells are separated out of the grafted liver and obtained. According to the method for separating the hepatic stellate cells, the yield and survival rate of the hepatic stellate cells can be increased, and the purity of HSCs can be guaranteed.

The invention discloses a sheepskin mold preventive, belonging to a leather assistant. The sheepskin mold preventive comprises the following components in parts by mass: 80-120 parts of pure water, 200-238 parts of calcium chloride, 35-40 parts of calcium hydroxide, 110-135 parts of chloral hydrate, 50-65 parts of nitroethane, 150-180 parts of bromine, 450-500 parts of carbon tetrachloride and a right amount of anhydrous sodium sulfate. The sheepskin mold preventive is a halogenated nitroparaffin alcohol compound, can still keep antimicrobial activity in nonionic/anionic surfactants and protein hydrolysates, and can inhibit the sheepskin mold from generation.

The invention discloses a B.garinii polyclonal antibody and an application. A BSK-II culture medium is inoculated with a B.garinii strain for enlargement culture, and a somatic antigen is obtained after inactivation; after the obtained somatic antigen and an adjuvant are fully emulsified, a New Zealand white rabbit is immunized by using multi-point intradermal injection; the immunized New Zealand white rabbit is anesthetized with chloral hydrate, whole blood is solidified at the room temperature after being collected, and serum containing the B.garinii polyclonal antibody is subjected to centrifugal separation. The B.garinii polyclonal antibody prepared with the method has good specificity and high titer, can effectively detect Lyme disease spirochete and has brighter application prospect.

The invention relates to an immunoassay method and kit for the indirect detection of chloral hydrate. The invention is underpinned by a novel immunogen that produces an antibody that is specific for the chloral hydrate metabolite trichloroethanol glucuronide. Detection and quantification of trichloroethanol glucuronide has important applications in clinical toxicology, drug facilitated crime, water testing and solvent exposure.

A method for fixing the eyeball of a living mouse comprises the steps that 1, the mouse is anesthetized with 4.3% chloral hydrate based on the rate of 0.1ml/10g, and it can be observed that all muscles of the mouse relax, breathing is steady, and corneal reflex is blunt five minutes after anesthetization; 2, the limbs of the mouse are fixed to a surface plate with clips, and the area around the eyelid of the mouse is scrubbed with iodophor; 3, under an electron microscope, a handle of a mouse eyeball snare is held with the index finger and thumb of the left hand, the annular structure of the mouse eyeball snare is pressed down from top to bottom right facing the upper surface of the eyelid of the mouse, the eyeball of the mouse is trapped and fixed by the annular structure after the eyelid of the mouse is pushed away by the annular structure, the eyeball of the mouse protrudes upwards out of the plane of the annular structure at the moment, and the annular structure is pressed downwards continuously till veins on the sclera of the eyeball of the mouse are exposed clearly.

The invention provides a quality evaluation method of traditional Chinese medicine-fructus evodiae. The method comprises the following steps: different fructus evodiae samples are ground and screened, and fructus evodiae powder is obtained; the fructus evodiae powder is weighed, ground repeatedly with a chloral hydrate solution and transferred into volumetric flasks, glycerin is added, constant volume is reached with the chloral hydrate solution, and suspensions are obtained; the suspensions are drawn for slice production, and nonglandular hairs are observed under a microscope and counted; the average number of the nonglandular hairs in the fructus evodiae samples per milligram is counted, and the product with the average number larger than or equal to 543.83 per milligram is qualified. With adoption of the method, quality of the traditional Chinese medicine-fructus evodiae is judged by determining the micro-characteristic constant value of the non-glandular hairs in the different fructus evodiae samples, and one novel method for evaluating quality of fructus evodiae is provided. Compared with a method for identifying quality of fructus evodiae with a main active ingredient determining process, the method has the advantages of being simple and convenient and obtaining results more quickly; compared with a conventional method for identifying fructus evodiae on the basis of morphology microscopic characteristics, the quality evaluation method is high in accuracy.

The invention relates to a method for synthesizing 7-halogenoindoles.The method includes the steps that o-halogenated aniline, chloral hydrate and hydroxylamine hydrochloride are subjected to a Sandmeyer reaction to synthesize 7-halogenated indirubin; the 7-halogenated indirubin is dissolved through an organic solvent, the 7-halogenoindoles is obtained in a reduction reaction mode under the reducing agent condition, and the reducing agent is an alkali metal hydroboron system or a lithium aluminum hydride system or a lithium hydride system or a triethyl-silane system.The method has the advantages that the o-halogenated aniline, the chloral hydrate and the hydroxylamine hydrochloride serve as raw materials, and the 7-halogenated indirubin is prepared with the Sandmeyer isonitroso acetanilide synthesis method, and then is reduced through the reduction system to prepare 7-halogenoindoles; the method for preparing the 7-halogenoindoles from the 7-halogenated indirubin has the advantages that raw materials are easy to obtain, cost is low, the technology yield is high, the product purity is good, operation is easy and convenient, and the method is suitable for preparing the 7-halogenoindoles in a large-scale mode.

The invention relates to a method for making a permanent glass slide specimen of bryophyte. Gum Arabic, double distilled water, glycerin and chloral hydrate are mixed with stirring at normal temperature, and a liquid mounting medium is prepared; moss leaves or plants are placed in the double distilled water, after infiltration, the moss leaves or plants are placed in the center of a slide glass with a droplet of double distilled water, and the moss leaves or plants are observed under a 10x microscope for adjusting shapes; a piece of filter paper is used for absorbing double distilled water which is 1 millimeter or more far away from the specimen, till the circumference of the moss specimen is moistened without excessive double distilled water; a glass rod is used for dipping 1-2 droplets of the mounting medium, and the droplets are dropped on the moss specimen, a piece of cover glass is covered without bubbles contained, the specimen is placed at normal temperature till the mounting medium is dried and can fix the specimen without movement, a label is plastered on the slide glass, and the permanent glass slide specimen of bryophyte is prepared. Compared with prior art, the produced specimen has the advantages of good transparency, simple method, long preservation time limit, and good preservation effect of original state of the specimen.

The invention relates to a multifunctional compound fertilizer with a sterilization function. The compound fertilizer is prepared from components in parts by mass as follows: 18-22 parts of phosphorus pentoxide, 20-24 parts of triacontanol, 16-20 parts of magnesium sulfate, 14-18 parts of rice bran, 20-24 parts of gibberellin, 16-20 parts of fulvic acid, 14-18 parts of manganese sulfate, 20-24 parts of rose willows, 16-20 parts of sessile stemona roots, 14-18 parts of carbendazim, 20-24 parts of peptone, 16-20 parts of sodium ortho-nitrophenol, 16-20 parts of ammonium magnesium phosphate, 14-18 parts of amino acid powder, 20-24 parts of itaconic acid, 16-20 parts of mepiquat chloride, 14-18 parts of compound sodium nitrophenolate, 20-24 parts of fluroxypr-mepthyl, 16-20 parts of deltamethrin, 14-18 parts of volcanic ash, 20-24 parts of zeolite, 16-20 parts of sepiolite, 14-18 parts of chloral hydrate, 20-24 parts of polyglycerol, 16-20 parts of methyl carbamate and 16-20 parts of guanidine sulfate. The compound fertilizer is suitable for being used in the agriculture field, and the plant maturation time is shortened.

The invention provides a rat hepatic stellate cell separation method, which comprises the following steps of (a) injecting 10-percent chloral hydrate into the abdominal cavity for anaesthetizing a rat, and laparotomizing the abdominal cavity of the rat so as to expose the liver and the portal vein; (b) performing heparin intravenous injection, and preheating perfusion liquid for liver perfusion; (d) taking the liver subjected to sufficient perfusion, cutting the liver into pieces, and performing oscillation digestion in preheated digestion liquid; (e) filtering digested liver tissues through a multilayer sieve screen; performing low-speed centrifugation; sucking supernatant; performing centrifugation again to obtain cell precipitates; and performing once cleaning by cleaning liquid; (f) resuspending the obtained cell precipitates, and performing Nycoden gradient centrifugal separation; (g) cleaning the cells for many times, resuspending the cell precipitates by a complete culture medium, and performing inoculated culture; and (h) performing cell immunofluorescent identification on rat hepatic stellate cells. The rat hepatic stellate cell separation method provided by the invention can be used for obtaining rat hepatic stellate cells which have high yield, high purity, high activity and can be activated.