50 results about "Hydrated chloral" patented technology

The invention relates to a method for synthesizing 7-halogenoindoles.The method includes the steps that o-halogenated aniline, chloral hydrate and hydroxylamine hydrochloride are subjected to a Sandmeyer reaction to synthesize 7-halogenated indirubin; the 7-halogenated indirubin is dissolved through an organic solvent, the 7-halogenoindoles is obtained in a reduction reaction mode under the reducing agent condition, and the reducing agent is an alkali metal hydroboron system or a lithium aluminum hydride system or a lithium hydride system or a triethyl-silane system.The method has the advantages that the o-halogenated aniline, the chloral hydrate and the hydroxylamine hydrochloride serve as raw materials, and the 7-halogenated indirubin is prepared with the Sandmeyer isonitroso acetanilide synthesis method, and then is reduced through the reduction system to prepare 7-halogenoindoles; the method for preparing the 7-halogenoindoles from the 7-halogenated indirubin has the advantages that raw materials are easy to obtain, cost is low, the technology yield is high, the product purity is good, operation is easy and convenient, and the method is suitable for preparing the 7-halogenoindoles in a large-scale mode.

The preparation method of 2-amino-5-fluobenzoic acid uses 4-fluoroaniline as raw material, and includes the following steps: making said raw material and hydrated chloral and oxammonium hydrochloride implement condensation reaction to obtain N-(4-fluorophenyl)-2-(hydroxyimide) acetamide, then making cyclization in concentrated sulfuric acid to produce 5-fluoro-1H-indole-2,3-diketone, and finally, under the alkaline condition using oxydol to make oxidation so as to obtain the invented 2-amino-5-fluorobenzoic acid.

The invention discloses a method for simultaneously dyeing mycelial masses and starch in mycorhiza of arethusa, which comprises the following steps: taking a mycorhiza slice, placing on a glass slide, dripping chloral hydrate iodine solution dye, dyeing, then sucking away the redundant dye, further dripping aniline blue lactoglycerin solution dye, dyeing, then sucking away the redundant dye, and placing under an optical microscope for micro-observation, so that the starch in the mycorhiza is in dark brown and the fungal mycelial masses are in blue. By adopting the method, the mycelial masses and the starch in the mycorhiza can be simultaneously dyed, and the colonization characteristics of fungi of the mycorhiza in a root of the arethusa and the starch distribution characteristics can be simultaneously observed, thereby having important significance for revealing the relationship between digestion and formation of the fungi of the mycorhiza and accumulation and the digestion of a hostcarbohydrate.

The invention relates to an improved method for observing microscopic characteristics of spices. The method comprises the following steps: grinding spice elementary bodies by utilizing a grinder, and screening through a 80-mesh screen to obtain spice powder; putting a small amount of spice powder on a glass slide, dropwise adding 1-2 drops of a chloral hydrate solution, and standing; slowly heating by utilizing an alcohol burner until liquid on the glass slide cannot naturally flow, dropwise adding 1 drop of a glycerinum solution having a volume ratio of 1:1 and 1 drop of diluted iodine solution having a volume ratio of 1:1, uniformly mixing by utilizing a sharp tool, and covering cover glass; observing with a low-power objective to find objects, selecting an object which is highlighted to observe, and observing with a high-power objective to obtain the microscopic characteristics of spices. By combining low-concentration chloral hydrate permeabilization and iodine staining, the method can be used for quickly, comprehensively, clearly and accurately observing the microscopic characteristics of spices, especially spices having relatively high starch content, can avoid the problems of over permeabilization and easily separated crystal, and can remarkably reduce the dosage of reagent with low cost and safety.