Detection kit and detection method for rapidly detecting novel coronavirus

Abstract

The invention discloses a detection kit and a detection method for rapidly detecting a novel coronavirus. The kit comprises a group of detection test paper, a positive control, a detection result recording card and an instruction book which are arranged in an aluminum foil bag; the novel coronavirus rapid detection test paper is composed of a bottom plate, a sample area, a reaction area, latex forsealing, a color development layer and a water absorption area; a sample pad and a water absorption pad are arranged on the bottom plate and located on the two opposite sides of the bottom plate respectively, and the sample pad is suction filter paper or a glass fiber membrane and used for bearing an object to be detected; a nitrocellulose membrane is fixed on the bottom plate, and the water absorption pad is water absorption paper; the preparation method comprises the following steps: specifically capturing and combining a coronavirus protein antigen by adopting double enzyme-linked immunosorbent assay through ACE2 and CHOP double proteins; combining a CHOP reaction element in the test paper reaction area with biotin, connecting avidin with horse radish peroxidase, and rapidly a detection result through chemical color development by virtue of a biotin-avidin system, wherein the virus content can be accurately detected by virtue of a microplate reader.

Description

technical field [0001] The invention relates to the technical field of rapid antigen detection reagents, in particular to a rapid detection kit and detection method for novel coronavirus. Background technique [0002] 2019 novel coronavirus, namely "2019-nCoV". Coronaviruses are a large family of viruses known to cause colds as well as more serious illnesses such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The novel coronavirus is a new strain of coronavirus that has never been found in humans before. [0003] However, due to limited materials and testing methods, it is known that the qPCR method is required for the diagnosis of new coronaviruses, which is more difficult to operate and more expensive than other detection methods. As a result, the number of existing kits cannot meet the unified detection of a large number of citizens. At present, the number of suspected cases of new coronavirus remains high. Improving the curren...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid detection of novel coronavirus detection kit and detection method to solve the problems raised in the above-mentioned background technology that the operation is more difficult and the cost is higher compared with other detection methods; the detection method does not need to directly obtain For coronavirus antibodies, only the common ACE2 protein and CHOP protein are obtained, and the new coronavirus can be detected by "double enzyme-linked immunosorbent assay". Screening; reduce the risk of individual infection in the hospital; shorten the waiting time for patients and provide timely treatment; reduce the workload of designated hospitals

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