Method for detecting tetracycline (TET) based on colloidal gold (AuNPs) chromatography test strip of aptamer

A nucleic acid aptamer and colloidal gold test paper technology, applied in the field of analytical chemistry, can solve the problems of unsuitability for rapid detection, complex analysis process, poor selectivity, etc., and achieve the effect of preventing and controlling food safety incidents
CN106645679AInactive Publication Date: 2017-05-10JILIN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2017-05-10
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for detecting tetracycline (TET) based on a colloidal gold (AuNPs) chromatography test strip of aptamer. The method uses specific binding between colloidal gold and the aptamer and competitive binding capacity strength between the tetracycline and DNA (deoxyribonucleic acid) and the aptamer. The method comprises the following steps of synthesizing colloidal gold nanoparticles suitable for marking the aptamer; assembling and coupling the colloidal gold and the aptamer; assembling streptavidin with a DNA chain; preprocessing a sample pad and a gold pad; scribing lines on an NC membrane; assembling the test strip; detecting standard tetracycline; performing selectivity and specificity experiments; using the established colloidal gold test strip based on the aptamer to measure the tetracycline in a practical sample. The tetracycline detecting method provided by the invention has the advantages of having better selectivity and flexibility, being simple, economic and easy to operate and being more suitable for in-site quick detection.
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Description

technical field

[0001] The invention relates to the detection of tetracycline (TET) by colloidal gold (AuNPs) chromatographic test strips based on nucleic acid aptamer (aptamer), belonging to the technical field of analytical chemistry. Background technique

[0002] Tetracycline antibiotics, as a kind of broad-spectrum antibiotics, are widely used to control bacterial infections and play a pivotal role in the treatment of animal diseases. Among them, tetracycline has become the most commonly used class of antibiotics because of its high efficiency, broad spectrum and low cost. Tetracycline is not only widely used as a drug in the prevention and treatment of human and animal diseases, but also is widely used as a feed additive to improve feed utilization efficiency and promote animal growth.

[0003] However, the chemical properties of tetracycline are relatively stable and have a certain degree of persistence, resulting in its residue in the environment. A large number of s...

Claims

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