Aquatic pathogenic bacterium colony induction signaling molecule detecting method and special reagent kit thereof

Abstract

The invention discloses a detection method for quorum sensing signal molecules of aquatic pathogenic bacteria and a special kit thereof. The method includes carrying out the following experiments with the tested bacteria: 1) Streak culture with Agrobacterium tumefaciens A136 on a plate added with X-Gal or ONPG, and observe whether it shows blue; Streak culture on the plate, observe whether it shows purple; 3) Streak inoculate the test bacteria on the LB plate containing 500nM N-acylated homoserine lactone for culture, and then place Violet bacteria CV026 next to the test bacteria streak Stretch in parallel to observe whether the purple lightens or fades; 4) Streak and culture with Esc.coli pSB403 on the plate to observe whether it emits light; in the above experiments, if at least one of the experiments is positive, the tested bacteria produce N - Acylated homoserine lactone. The method of the invention has high accuracy rate, no false negative phenomenon, easy qualitative and quantitative analysis, simple and convenient operation, low cost, quick result, and is suitable for large-scale high-efficiency detection.

Description

technical field [0001] The invention relates to a rapid detection method for quorum sensing signal molecules of aquatic Gram-negative pathogenic bacteria and a special kit thereof. Background technique [0002] The economic loss of aquaculture caused by bacterial diseases in my country is as high as 50 billion yuan every year, and aquatic pathogens are mainly Gram-negative bacteria (Jiang Lan, et al. Chinese Fishery Science. 9 (2): 152-156, 2002), common Include Vibrio anguillarum, Aeromonas salmonicida, Aeromonas hydrophila, Yersiniaruckeri, Vibrio salmonicida, Vibrio vulnificus, Aeromonas salmonicida and Vibrio splendidus etc. It is related and should be distinguished from the phytopathogenic bacteria that have been studied more at present (Gao Yijing, Master's Degree Thesis of Nanjing Agricultural University, Effects of the Quorum Sensing System of Mesobrachytropha Rhizobium on its Growth and Symbiotic Nodulation, 2006). The use of conventional chemicals and antibiotics ha...

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Problems solved by technology

However, the published biological reporting system is relatively simple (Zhan Kehang, et al. Chinese Medical Journal. 86(37): 2655-2658, 2006), there are certain limitations in the detection range and accuracy, and there will be false negative reports (Yang Menghua, et al. Acta Microbiology. 46(6): 1007-1010, 2006)

The identification of quorum-sensing signaling molecules in aquatic Gram-negative pathogens has not been reported

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