Kit for jointly detecting breast cancer 21 genes and preparation method of kit

Abstract

The invention discloses a kit for jointly detecting breast cancer 21 genes. A PCR (polymerase chain reaction) amplification primer consists of 21 primer pairs, and can be used for jointly detecting 21 gene-related expression quantities: Ki67, STK15, Survivin, CCNB1, MYBL2, MMP11, CTSL2, GRB7, HER2, GSTM1, CD68, BAG1, ER, PGR, BCL2, SCUBE2, ACTB, GAPDH, PRLPO, GUS, and TFRC. Output fragments of the 21 pairs of primers related in the invention are smaller than 100bp, so that PCR efficiency after reverse transcription of mRNA (messenger ribonucleic acid) extracted by a paraffin specimen is greatly improved, all primers of 21 genes are premixed, and time and labor for designing and synthesizing 21 pairs of genes by an experimenter are saved; the corresponding primers of the 21 genes are not needed to be added for premixing in mother liquor, and therefore, errors of reaction holes are greatly reduced. The kit disclosed by the invention is simple to operate, and capable of detecting on a quantitative PCR instrument by only adding a template, so that detection experimental time is greatly shortened. The kit disclosed by the invention is applicable to a single sample and multiple samplers, so that results can be quickly obtained, and therefore, scientific research and clinical detection requirements can be satisfied very well.

Description

technical field [0001] The invention belongs to the field of medical molecular biology and relates to a kit, in particular to a kit for combined detection of breast cancer 21 genes. Background technique [0002] In recent years, with the in-depth study of genomics, the "21 gene detection" (Oncotype Dx) for estrogen receptor positive patients has been proved to be more accurate than clinicopathological indicators in predicting estrogen receptor positive, axillary lymph node Negative and reduce the risk of distant metastasis in patients taking tamoxifen. The test is the only multigene test recommended jointly by the American Society of Clinical Oncology (ASCO) and National Comprehensive Cancer Network (NCCN) guidelines. The 21 genes included 16 tumor-related genes and 5 reference genes. Tumor-related genes include: proliferation-related genes (Ki-67, STK15, Survivin, Cyclin B1, MYBL2), invasion-related genes (Stromelysin3, Cathepsin L2), Her-2-related genes (GRB7, Her-2), ho...

AI Technical Summary

Problems solved by technology

However, the PCR reaction itself is a highly sensitive experiment, and so many genes are combined into reaction wells, which are prone to errors in the detection experiment operation, and the operation process is cumbersome and time-consuming.

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