Efficient chlorobenzene degradation obligate bacterium, composite microbial agent and preparation method and application of composite microbial agent

A technology of composite microbial agent and composite strain, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unstable operation, high technical requirements, and long microbial domestication cycle, and achieve shortened degradation The time required, beneficial to long-term storage, and the effect of improving the degradation rate

Active Publication Date: 2017-04-26
YANCHENG INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are usually disadvantages such as long microbial domestication cycle, high technical requirements, long start-up time of the waste gas biological treatment system, low removal efficiency during the start-up period, and unstable operation, especially in the biological purification process for insoluble and refractory waste gas. protrude
In addition, sludge activity is not easy to preserve, and it is inconvenient to carry and transport

Method used

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  • Efficient chlorobenzene degradation obligate bacterium, composite microbial agent and preparation method and application of composite microbial agent
  • Efficient chlorobenzene degradation obligate bacterium, composite microbial agent and preparation method and application of composite microbial agent
  • Efficient chlorobenzene degradation obligate bacterium, composite microbial agent and preparation method and application of composite microbial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add 250mg, 500mg, 1000mg, 1500mg, and 2000mg of chlorobenzene as enrichment medium to five 1L inorganic salt medium respectively, wherein the inorganic salt medium includes a mass ratio of 3:1:1.5:0.1:0.03:0.005 :K of 0.01 2 HPO 4 、KH 2 PO 4 , NH 4 Cl, MgSO 4 , MnSO 4 , FeSO 4 ·7H 2 O and CaCl 2 2H 2 O, and chlorobenzene was added therein so that the mass ratio of carbon, nitrogen, and phosphorus in the medium was 90:3:1; respectively, 1 mL of the enriched culture solution in the above-mentioned enriched medium was applied to the inorganic salt medium And add the bacterial strain isolated from the biofilm on the surface of the biotrickling filter packing, culture at 30°C for 1d, pick the grown colony into a 96-well plate equipped with 100μL inorganic salt medium, cultivate it for 0.5d, and use A 4mm sterile filter paper sheet absorbs the culture solution and pastes it on the identification medium. The identification medium is formed by adding 1500mg chlorobenz...

Embodiment 2

[0040] Add 250mg, 500mg, 1000mg, 1500mg, and 2000mg of chlorobenzene as enrichment medium to five 1L inorganic salt medium respectively, wherein the inorganic salt medium includes a mass ratio of 5:3:3:0.3:0.08:0.015 :K of 0.05 2 HPO 4 、KH 2 PO 4 , NH 4 Cl, MgSO 4 , MnSO 4 , FeSO 4 ·7H 2 O and CaCl 2 2H 2 O, and chlorobenzene was added therein so that the mass ratio of carbon, nitrogen, and phosphorus in the medium was 110:7:1; respectively, 1 mL of the enriched culture solution in the above-mentioned enriched medium was applied to the inorganic salt medium And add the strain isolated from the biofilm on the surface of the biotrickling filter packing, culture at 45°C for 5 days, pick the grown colony into a 96-well plate with 200 μL of inorganic salt medium, cultivate it for 3 days, and then use 8mm After absorbing the culture solution, paste it on the identification medium. The identification medium is formed by adding 2500mg chlorobenzene and 20g agar to 1L inorgan...

Embodiment 3

[0045] Add 250mg, 500mg, 1000mg, 1500mg, and 2000mg of chlorobenzene to five 1L inorganic salt mediums as enrichment medium, wherein the inorganic salt medium includes a mass ratio of 4:2:2.2:0.2:0.05:0.01 :K of 0.03 2 HPO 4 、KH 2 PO 4 , NH 4 Cl, MgSO 4 , MnSO 4 , FeSO 4 ·7H 2 O and CaCl 2 2H 2 O, and chlorobenzene was added therein so that the mass ratio of carbon, nitrogen, and phosphorus in the medium was 100:5:1; respectively, 1 mL of the enriched culture solution in the above-mentioned enriched medium was applied to the inorganic salt medium And add the bacterial strain isolated from the biofilm on the surface of the biotrickling filter packing, culture at 37°C for 3 days, pick the grown colony into a 96-well plate containing 150 μL of inorganic salt medium, cultivate it for 1.7 days, and use A 6mm sterile filter paper sheet absorbs the culture solution and pastes it on the identification medium, which is formed by adding 2000mg chlorobenzene and 15g agar to 1L ...

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Abstract

The invention relates to an efficient chlorobenzene degradation obligate bacterium, a composite microbial agent and a preparation method and application of the composite microbial agent, and belongs to the technical field of waste gas bio-purification. The efficient chlorobenzene degradation obligate bacterium is entitled a biological collection number: CGMCC No. 10053. The preparation method of the composite microbial agent comprises: mixing the efficient chlorobenzene degradation obligate bacterium with mixed bacteria at a mass ratio of (0.5-1):1 to obtain a composite strain, and inoculating and fermenting the composite strain until the viable count of fermentation broth ranges from 1.0 * 1010 cuf / mL to 1.0 * 1011 cfu / mL; mixing the fermentation broth and a solid carrier material at a ratio by weight of 1:(1-10) to obtain a composite microbial agent; the mixed bacteria are domesticated from activated sludge in an aeration tank of a wastewater treatment station of a petrochemical plant. The preparation method is simple and easy to perform; the composite microbial agent prepared by using the method is directly applicable to the startup of a waste gas bio-treatment system, is effective for degrading chlorobenzene waste gas and is easy to store and convenient to carry.

Description

technical field [0001] The invention relates to the technical field of waste gas biological purification, and in particular to a chlorobenzene-efficient obligatory bacterium for degrading chlorobenzene, a composite microbial bacterial agent and a preparation method and application thereof. Background technique [0002] According to reports, about 1.5 million tons of chlorobenzene organic compounds are released into the atmosphere every year. Chlorobenzene organic compounds account for more than half of the 129 priority pollutants listed by the U.S. Environmental Protection Agency (EPA). my country has also listed chlorobenzene as a blacklist of key pollutants. Most of the chlorobenzene organic compounds are toxic, and some of them are considered to have carcinogenic, teratogenic and mutagenic effects. There is no safe threshold dose of carcinogenic chlorobenzenes, so any exposure, no matter how small the dose, indicates a certain risk. The release of chlorobenzenes also i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/02C12N11/14B01D53/84B01D53/70C12R1/07
CPCB01D53/70B01D53/84B01D2251/95B01D2257/2064C12N1/20C12N11/02C12N11/10C12N11/14C12N1/205C12R2001/07Y02A50/20
Inventor 杨百忍丁成李朝霞许琦孙祝秋
Owner YANCHENG INST OF TECH
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