Golden Orange II Degrading Bacteria l-15 and the Bacteria Produced by It

A technology for degrading bacteria and bacterial agents, applied in the biological field, can solve problems such as pollution, the development of azo dye Golden Orange II has not seen a relatively mature technology, and comprehensive management of harm to human beings. It is convenient to use, protects human health, The effect of low cost of production and use

Active Publication Date: 2016-05-25
苏州鑫泷生态环境有限公司
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Problems solved by technology

[0006] Azo dye golden orange II products are soluble in water, the pollution of the environment by azo dye golden orange II has seriously endangered the health of human beings, the comprehensive treatment of its environment, the development of degrading bacteria of azo dye golden orange II in the environment There is no relatively mature technology

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  • Golden Orange II Degrading Bacteria l-15 and the Bacteria Produced by It
  • Golden Orange II Degrading Bacteria l-15 and the Bacteria Produced by It
  • Golden Orange II Degrading Bacteria l-15 and the Bacteria Produced by It

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Embodiment Construction

[0020] The present invention will be further analyzed below in conjunction with specific embodiments and accompanying drawings.

[0021] Golden Orange II degrading bacteria, whose strain is Stenotrophomonas maltophilia strain L-15, was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on June 11, 2014, address: Beichen West, Chaoyang District, Beijing Road No. 1 Courtyard, collection number CGMCCNO.9289.

[0022] 1. Isolation and identification of strains

[0023] Take 10g of contaminated soil, put it in 100mL of sterile water, and shake it for 5min to get the soil bacteria suspension; take 5mL of the soil bacteria suspension, add it to 100mL of inorganic salt medium [NH 4 NO 3 : 407mg / L; KH 2 PO 4 : 98mg / L; K 2 HPO 4 : 33mg / L; NaCl: 30mg / L; MgSO 4 : 200mg / L; pH7.0], add Golden Orange II as a carbon source, place it at 30°C, and culture it on a shaker at 150r / min to obtain a rich solution, which is continuously spre...

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Abstract

The invention discloses orange II degrading bacterium L-15 and an inocula produced from the same. The orange II degrading bacterium L-15 has the bacterial strain of stenotrophomonas maltophilia, belongs to xanthomonas campestris of xanthomonadaceae, and is preserved in China General Microbiological Culture Collection Center since 11st, June, 2014, the preservation address is No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC NO.9289. The main biological characteristics comprise that the bacterium colony is circle and is not transparent, and degrading bacterium L-15 is obligate aerobic non-fermentation type Gram negative polar flagella bacillus, has strong ammonia flavor on a blood agar plate and has no hemolysis phenomenon. Degrading bacterium L-15 is capable of reducing the residual quantity of the azo dye orange II by 80% or more. The inocula has the advantages of low production usage cost, usage convenience and good removing effect, and is applicable to pollution treatment of water and soil.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to golden orange II degrading bacteria L-15 and the bacterial agent produced therefrom, which degrades dyes by means of microorganisms and is suitable for the treatment of environmental pollution. Background technique [0002] Azo dyes (a class of organic compounds in which the two ends of the azo group are connected to aryl groups) are the most widely used synthetic dyes in the printing and dyeing process of textiles and clothing. They are used for dyeing and printing of various natural and synthetic fibers. Coloring of paint, plastic, rubber, etc. Under special conditions, it can decompose and produce more than 20 kinds of carcinogenic aromatic amines, which can change the DNA structure of the human body through activation to cause pathological changes and induce cancer. [0003] The common feature of azo compounds is that the molecule contains an azo bond "—N=N—"...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
Inventor 刘秀艳焦亮郭军史晓亮
Owner 苏州鑫泷生态环境有限公司
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