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Method for cultivating wide spectrum pest-resistant transgene rape

A breeding method and transgenic technology, applied in the direction of genetic engineering, plant genetic improvement, botanical equipment and methods, etc., to achieve low cost, high efficiency, and long-lasting control effect

Inactive Publication Date: 2008-08-06
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the literature search, there is no patented technology or non-patent literature published or used at home and abroad that targets the plant endogenous myrosinase gene and uses transgenic technology to create broad-spectrum insect-resistant crop varieties.

Method used

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  • Method for cultivating wide spectrum pest-resistant transgene rape

Examples

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Embodiment 1

[0027] Example 1 The use of antisense RNA technology to create broad-spectrum insect-resistant transgenic rapeseed

[0028] Referring to Fig. 1 as can be known, a kind of method of cultivating broad-spectrum insect-resistant transgenic rapeseed comprises the steps:

[0029] (1) Construction of antisense RNA expression vector:

[0030] The sources of Arabidopsis thaliana enzyme genes TGG1 and TGG2 can be found in literature (Xue et al., The myrosinasegene family in Arabidopsis thaliana: gene organization, expression and evolution, Plant Molecular Biology, 1995, 27(5): 911-922). In the following experimental steps, unless otherwise specified, all operations were carried out in accordance with the methods provided in "Molecular Cloning Experiment Guide" (Third Edition) (translated by Huang Peitang et al., Beijing: Science Press, 2002). The reagents used for restriction endonuclease, pGEM-T Vector, RNA reverse transcription, and RT-PCR were purchased from Promega Company, the RNA...

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Abstract

The invention discloses a cultivating method of a wide spectrum pest-resistant transgenic rape, which comprises the following steps: firstly, obtaining object gene order, secondly, constructing antisense RNA expression vector, thirdly, doing genetic transformation, fourthly, testing transgenic plant, fifthly, identifying insect resistance, and finally, culturing insect resistance variety. The invention stops the translation of plant endogenous myrosin gene, eliminates or lowers the synthesis and activity of myrosin, complete glucosinolates in the bodies of plant can not be degraded, volatile chemical signal substance which can be identified by obligate pest can not be formed, thereby eliminating identification signal of obligate pest to crops, avoiding pest damage, or oviposition and breeding are inhibited even if feeding, thereby pests are effectively controlled. The cultivating method has permanent effect, wide scope and low cost, which has no public hazard.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering of cruciferous crops, in particular to a method for cultivating cruciferous crops such as broad-spectrum insect-resistant transgenic rapeseed, using antisense RNA or RNAi technology to interfere with the translation of plant endogenous myrosinase genes, Inhibit the degradation of complete glucosinolate glucoside in plants, block the signal recognition of cruciferous crops by obligate pests, and are suitable for the genetic improvement of insect resistance in rapeseed (Brassica napus, Brassica napus and Brassica napus). Suitable for other cruciferous vegetable crops such as cabbage, kale and radishes. Background technique [0002] Cruciferous crops such as rapeseed and cabbage, cabbage and radish are closely related to people's life. These crops are vulnerable to pests during the growing season, causing huge economic losses and a serious decline in quality. In order to control pests a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/56C12N15/82A01H1/00
Inventor 伍晓明陆光远牛莉娜高桂珍陈碧云许鲲
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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