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Method for prolonging in vitro preservation duration of blumeriagraminisf .sp .tritici

A technique for ex vivo preservation of wheat powdery mildew, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of multiple workloads, consumption of conidia, and prolongation of conidia of wheat powdery mildew In vitro storage time and other issues to achieve the effect of improving work efficiency and saving labor

Active Publication Date: 2018-10-12
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Description
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Problems solved by technology

[0003] In recent years, some related research has been carried out on the long-term preservation of powdery mildew bacteria in vitro. For example, Yu Dazhao et al. disclosed in the patent "a long-term preservation method for wheat powdery mildew strains (CN201310180918.4)", which uses silica gel drying and low-temperature freezing. The preservation method can be preserved for a long time for 1 year, but there are two obvious deficiencies in this method: 1) the preservation time is short and can only be preserved for 1 year; Spores take more work
However, in this invention, it was not found that the electronic fluoride solution can prolong the in vitro storage time of conidia of wheat powdery mildew

Method used

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Effect test

Embodiment 1

[0014] The impact of conidia quality on preservation effect in the improved method of embodiment 1

[0015] According to the method of the present invention, the resurrection rate of bacterial strains under different conidia masses was compared, and a total of 20 bacterial strains were preserved, and the storage time was 1 year respectively, and conventional methods were used as a control. The detection of pathogenicity shows that the revival rate of the strain is 100% when the amount of spores is 5mg in the improved method; The resurrection rate of the bacterial strain was 100%; when the amount of conidia was 5.0mg, the resurrection rate of the bacterial strain was 0%. The specific results are shown in Table 1.

[0016] Table 1 Comparison of strain resurrection rate under different conidia quality

[0017]

Embodiment 2

[0018] Embodiment 2 improved method preservation time effect

[0019] Compare routine and the resurrection rate of the method of the present invention to the isolated conidia of wheat powdery mildew bacteria: save 20 bacterial strains in total, and the storage time is respectively 1, 3 and 6 years, preserve with conventional method as contrast, the different storage time The conidia of wheat powdery mildew were suspended in water (preserved by conventional methods), suspended with C5-18-perfluoroalkane (FC-40), and finally the spore suspension of wheat powdery mildew was sprayed and inoculated on wheat leaves to investigate the incidence Happening. The mass of conidia powder suspended in water (preserved by conventional methods) is 20 mg, and the mass of conidia powder suspended in C5-18-perfluoroalkane (FC-40) is 5 mg.

[0020] Show by pathogenicity detection, routine method (use the spore that water suspends in vitro preservation, shaking powder or blowing inoculation metho...

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Abstract

The invention discloses a method for prolonging in vitro preservation duration of blumeriagraminisf .sp .tritici, and belongs to the technical field of application of preservation methods of plant pathogen obligate parasites. The blumeriagraminisf .sp .tritici, which belongs to plant pathogen obligate parasites, can survive with the existing of wheat leaf hosts. A method, discovered by the inventor, can achieve in vitro preservation of the blumeriagraminisf .sp .tritici without the assistance of hosts, however, the method has the shortcomings that the method is short in preservation duration,the method needs to collect a great amount of conidia and the like; on the basis, a strain resurrection inoculation mode is adopted, and blumeriagraminisf .sp .tritici spores undergo in vitro preservation through suspension of a perfluoroalkane solution, so that in vitro preservation duration of the blumeriagraminisf .sp .tritici spores is improved by six times in comparison with that of an original method, namely the in vitro preservation duration is prolonged to six years from original one year, and the mass of the required spores is reduced by four times. With the application of the methodprovided by the invention, shortcomings of existing methods can be overcome; and smooth implementation of related researches on blumeriagraminisf .sp .tritici pathogenic bacteria can be fully guaranteed.

Description

technical field [0001] The invention belongs to the application technical field of a method for preserving plant pathogenic obligate parasites, and in particular relates to a method for improving the in vitro preservation time of wheat powdery mildew. Background technique [0002] Wheat powdery mildew is a fungal disease caused by powdery mildew (Blumeria graminisf.sp.tritici, Bgt). The seedlings are carried out every 10 days or so; in addition, the propagation and collection of wheat powdery mildew conidia takes a long time and requires a lot of work. On the other hand, cross-contamination often occurs, resulting in the loss of specific pathogenic strains. Therefore, the establishment of a long-term preservation method can be used for research on genetic diversity of pathogen populations and molecular drug resistance monitoring. [0003] In recent years, some related research has been carried out on the long-term preservation of powdery mildew bacteria in vitro. For exampl...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N3/00C12R1/645
CPCC12N1/04C12N3/00
Inventor 杨立军龚双军薛敏锋向礼波史文琦曾凡松刘美玲陈婷婷喻大昭
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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