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A method for increasing the storage time of wheat powdery mildew in vitro

A technology for ex vivo preservation of wheat powdery mildew, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of multiple workloads, consumption of conidia, and short storage time, etc. Achieve the effect of improving work efficiency and saving labor force

Active Publication Date: 2022-02-18
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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Problems solved by technology

[0003] In recent years, some related research has been carried out on the long-term preservation of powdery mildew bacteria in vitro. For example, Yu Dazhao et al. disclosed in the patent "a long-term preservation method for wheat powdery mildew strains (CN201310180918.4)", which uses silica gel drying and low-temperature freezing. The preservation method can be preserved for a long time for 1 year, but there are two obvious deficiencies in this method: 1) the preservation time is short and can only be preserved for 1 year; Spores take more work
However, in this invention, it was not found that the electronic fluoride solution can prolong the in vitro storage time of conidia of wheat powdery mildew

Method used

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  • A method for increasing the storage time of wheat powdery mildew in vitro
  • A method for increasing the storage time of wheat powdery mildew in vitro

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Effect test

Embodiment 1

[0014] The impact of conidia quality on preservation effect in the improved method of embodiment 1

[0015] According to the method of the present invention, the resurrection rate of bacterial strains under different conidia masses was compared, and a total of 20 bacterial strains were preserved, and the storage time was 1 year respectively, and conventional methods were used as a control. The detection of pathogenicity shows that the revival rate of the strain is 100% when the amount of spores is 5mg in the improved method; The resurrection rate of the bacterial strain was 100%; when the amount of conidia was 5.0mg, the resurrection rate of the bacterial strain was 0%. The specific results are shown in Table 1.

[0016] Table 1 Comparison of strain resurrection rate under different conidia quality

[0017]

Embodiment 2

[0018] Embodiment 2 improved method preservation time effect

[0019] Compare routine and the resurrection rate of the method of the present invention to the isolated conidia of wheat powdery mildew bacteria: save 20 bacterial strains in total, and the storage time is respectively 1, 3 and 6 years, preserve with conventional method as contrast, the different storage time Wheat powdery mildew conidia were suspended in water (preserved by conventional methods), suspended with C5-18-perfluoroalkane (FC-40), and finally the wheat powdery mildew spore suspension was sprayed and inoculated on wheat leaves to investigate the incidence Condition. The mass of conidia powder suspended in water (preserved by conventional methods) is 20 mg, and the mass of conidia powder suspended in C5-18-perfluoroalkane (FC-40) is 5 mg.

[0020] Show by pathogenicity detection, routine method (use the spore that water suspends in vitro preservation, shaking powder or blowing inoculation method) is pres...

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Abstract

The invention discloses a method for improving the in vitro preservation time of wheat powdery mildew, and belongs to the application technical field of the method for preserving plant pathogenic obligate parasites. Wheat powdery mildew is a phytopathogenic obligate parasite, which requires the presence of wheat leaf hosts to survive. Although the inventors have discovered a method for in vitro preservation of wheat powdery mildew without a host, this method has a short storage time and needs to be collected. Shortcomings such as a large amount of conidia; The present invention has changed bacterial strain resurrection inoculation mode on this basis, uses perfluoroalkane solution to suspend the wheat powdery mildew spore of ex vivo preservation, and the in vitro preservation time of wheat powdery mildew spore is improved 6 times than original method times, extended from 1 year to 6 years, and the required spore mass was reduced by 4 times. This method overcomes the deficiencies in the existing methods, and can fully ensure the smooth development of related research work on wheat powdery mildew pathogens.

Description

technical field [0001] The invention belongs to the application technical field of a method for preserving plant pathogenic obligate parasites, and in particular relates to a method for improving the in vitro preservation time of wheat powdery mildew. Background technique [0002] Wheat powdery mildew is a fungal disease caused by powdery mildew (Blumeria graminisf.sp.tritici, Bgt). The seedlings are carried out every 10 days or so; in addition, the propagation and collection of wheat powdery mildew conidia takes a long time and requires a lot of work. On the other hand, cross-contamination often occurs, resulting in the loss of specific pathogenic strains. Therefore, the establishment of a long-term preservation method can be used for research on genetic diversity of pathogen populations and molecular drug resistance monitoring. [0003] In recent years, some related research has been carried out on the long-term preservation of powdery mildew bacteria in vitro. For exampl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12N3/00C12R1/645
CPCC12N1/04C12N3/00
Inventor 杨立军龚双军薛敏锋向礼波史文琦曾凡松刘美玲陈婷婷喻大昭
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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