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HClO detection microelectrode and preparation method and application thereof

A micro-electrode and electrode technology, which is applied in the field of HClO sensor and its preparation, can solve problems such as interference of biologically related substances, achieve fast response time, high selectivity, and improve detection accuracy

Active Publication Date: 2020-08-11
SHANGQIU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the electrochemical detection based on HClO is mainly based on two effects: (1) rely on the electrochemical oxidation of HClO itself, and reduce its oxidation overpotential by regulating the characteristics of the electrode material, but unfortunately, common biologically related substances How to achieve high selectivity detection is a big challenge
(2) In order to meet the requirements of practical applications and avoid measurement errors caused by complex sample environments or sample dilution, how to achieve high-accuracy electrochemical detection is another major challenge

Method used

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  • HClO detection microelectrode and preparation method and application thereof
  • HClO detection microelectrode and preparation method and application thereof
  • HClO detection microelectrode and preparation method and application thereof

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Embodiment 1

[0042] Embodiment 1: The preparation method of HClO detection microelectrode CFME / CNT / ABTS+MBS

[0043] (1) Synthesis of HClO-specific recognition molecular probes

[0044] First, methylene blue (1.12g, 3.75mmol) and Na 2CO 3 (1.59 g, 15.00 mmol) was dissolved in 5 mL of water, followed by the addition of dichloromethane (10 mL), and the mixture was stirred at 40°C under nitrogen. Sodium dithionite (2.61 g, 15.00 mmol) was then dissolved in 5 mL of water and injected directly into the solution using a syringe. After the addition was complete, the mixture was stirred at 40 °C under nitrogen until the solution turned yellow (30 min). The dichloromethane layer was separated from the aqueous layer and dried quickly over anhydrous sodium sulfate. After the sodium sulfate was removed by filtration, the above filtrate was quickly added to triethylamine (0.5mL). In an ice-water bath, under nitrogen, a solution of isobutyryl chloride (3.75mmol) was added dropwise to the mixture and...

Embodiment 2

[0047] Embodiment 2: HClO detection microelectrode CFME / CNT / ABTS+MBS is to the determination of HClO

[0048] The microelectrode CFME / CNT / ABTS+MBS prepared by the present invention is the working electrode, with Ag / AgCl as the reference electrode, and the Pt wire as the counter electrode, and measuring the SWV response before and after adding 50 μM HClO in the 0.1M PBS solution, the potential window: -0.5~0.8V. The result is as Figure 4 , when there is no HClO, there is only one oxidation peak at 0.53V, which is attributed to the electrochemical signal of ABTS. When HClO is added, a new peak appears at around -0.30V, which is mainly due to the reaction between HClO and MBS to form methyl The electrochemical signal generated by the blue structure, in which ABTS is used as an internal reference to improve the accuracy of electrochemical sensing, and MBS is used as a specific recognition molecule of HClO to improve the selectivity of detection. The reaction mechanism is determ...

Embodiment 3

[0049] Embodiment 3: anti-interference experiment

[0050] The CHI660E electrochemical workstation was used to measure the interference of the HClO detection microelectrode CFME / CNT / ABTS+MBS prepared in the present invention. Such as Figure 9 As shown, adding other biologically common active molecules (active oxygen, active sulfur and active nitrogen, etc.) High selectivity.

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Abstract

The invention discloses a microelectrode CFME / CNT / ABTS + MBS for detecting HClO in trace blood and a preparation method and application thereof, and belongs to the technical field of application of organic molecular probes in biosensing. The preparation method comprises the following steps: firstly, synthesizing an electrochemical probe MBS with HClO specific recognition, and co-modifying MBS molecules and ABTS molecules with an internal reference effect on a CFME / CNT electrode through pi-pi bonds to prepare the HClO detection microelectrode CFME / CNT / ABTS + MBS. The microelectrode CFME / CNT / ABTS + MBS has high selectivity on the response of HClO, and the introduction of the internal reference also improves the detection accuracy and anti-pollution ability of an actual sample. The HClO microelectrode can realize qualitative or quantitative determination of HClO in a trace blood sample, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of the application of organic probes in biosensing, and relates to a sensor for specific recognition and high-accuracy detection of HClO in trace blood, a preparation method and application thereof. Background technique [0002] As a kind of active oxygen, HClO plays an important role. In daily life, HClO is used for sterilization and disinfection; in biological systems, HClO is an important part of our immune system, especially neutrophils as the first line of host defense against malignant tumors and invading pathogens. However, the abnormal content of HClO will lead to "oxidative stress", which will further cause many diseases related to inflammation, such as neurodegenerative diseases, atherosclerosis, cancer, etc. Therefore, whether it is in daily life or in physiological systems, it is very necessary to detect the content of HClO. [0003] So far, many methods have been developed for the detection of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D279/30G01N27/30G01N27/48
CPCC07D279/30G01N27/308G01N27/48
Inventor 董辉周艳丽赵乐何帅郝远强张银堂徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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