Quick detection method for protein in milk

A detection method and protein technology, which is applied in the field of rapid detection of protein in milk, can solve problems such as milk adulteration, and achieve the effect of quantitative determination

Inactive Publication Date: 2017-07-25
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Protein content is a key indicator to measure the quality of milk. Some unscrupulous vendors use the "National Food Safety Standard - Determination of Protein in Food (GB / T 5009.5-2003)" to detect inorganic nitrogen-containing substances and organic non-protein nitrogen-containing substances. Insufficient positive, by adding melamine, ammonium chloride, urea and other non-protein nitrogen to milk to increase the nitrogen content of the product, resulting in milk adulteration incidents from time to time

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  • Quick detection method for protein in milk

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Effect test

Embodiment 1

[0016] A rapid detection method for protein in milk, comprising the steps of:

[0017] (1) With the sulfuric acid aqueous solution of 0.5mol / L as solvent, the sunset yellow solution that preparation mass concentration is 0.1% is detection reagent;

[0018] (2) Prepare standard protein aqueous solutions with mass concentrations of 1.0%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, and 5.0%, and mix standard protein aqueous solutions with detection reagents at a ratio of 10:1 by volume Mix, shake and react for 1 min, centrifuge, take the supernatant and dilute 10 times with water, measure the absorbance at 483nm with a UV-visible spectrophotometer, and draw a standard curve ( figure 1 );

[0019] (3) According to the volume ratio of 10:1, mix a commercially available brand of milk with the detection reagent, oscillate for 1 minute and then centrifuge, take the supernatant and dilute it 10 times with water, and measure the absorbance at 483nm with a UV-visible spectrophotometer. According to ...

Embodiment 2

[0021] A rapid detection method for protein in milk, comprising the steps of:

[0022] (1) With the hydrochloric acid aqueous solution of 0.1mol / L as solvent, the sunset yellow solution that preparation mass concentration is 0.005% is detection reagent;

[0023] (2) prepare the standard protein aqueous solution of series concentration, be 1.5%, 2.0%, 2.5%, 3.0%, the standard protein aqueous solution of 3.5% as mass concentration, be the ratio of 15:1 by volume, respectively standard protein aqueous solution and Mix the detection reagents, shake and react for 0.5min, centrifuge, take the supernatant and dilute 10 times with water, and prepare a standard color scale card according to the color of the solution;

[0024] (3) According to the volume ratio of 15:1, mix a commercially available brand of milk with the detection reagent, oscillate for 0.5 minutes, centrifuge, take the supernatant and dilute it 10 times with water, and compare it with the standard color scale card to ob...

Embodiment 3

[0026] A rapid detection method for protein in milk, comprising the steps of:

[0027] (1) with the nitric acid aqueous solution of 1.0mol / L as solvent, the sunset yellow solution that preparation mass concentration is 0.15% is detection reagent;

[0028] (2) prepare the standard protein aqueous solution of series concentration, be 1.5%, 2.0%, 2.5%, 3.0%, 3.5% standard protein aqueous solution as mass concentration, be the ratio of 8:1 by volume, respectively standard protein aqueous solution and Mix the detection reagents, shake and react for 10 minutes, centrifuge, take the supernatant and dilute 10 times with water, and prepare a standard color scale card according to the color of the solution;

[0029] (3) According to the volume ratio of 8:1, mix a commercially available brand of milk with the detection reagent, shake the reaction for 10 minutes, centrifuge, take the supernatant and dilute it 10 times with water, and compare it with the standard color scale card to obtain...

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Abstract

The invention discloses a quick detection method for protein in milk. The method comprises the following steps: (1) preparing a sunset yellow solution as a detecting reagent; (2) preparing a series of standard protein aqueous solutions in concentrations, mixing the standard protein aqueous solutions with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, preparing a standard color gradation card according to the solution color or using an ultraviolet and visible spectrophotometer for determining absorbancy at 483nm and drawing a standard curve; (3) mixing a to-be-detected milk sample with the detecting reagent, oscillating, centrifuging, taking supernate and diluting with water, comparing with the standard color gradation card or determining absorbancy at 483nm using the ultraviolet and visible spectrophotometer and calculating the content of the protein in the to-be-detected milk sample according to the standard curve. According to the method disclosed by the invention, the quick detection for the content of the protein in milk can be completed within 15-20min. When the method is adopted together with an ultraviolet spectrophotometer, the quantitative determination for the content of the protein in milk can be realized.

Description

technical field [0001] The invention relates to a rapid detection method for protein in milk. Background technique [0002] With the rapid development of my country's economy, people's demand for milk and other foods is increasing, and its quality and safety have also attracted much attention. Protein content is a key indicator to measure the quality of milk. Some unscrupulous vendors use the "National Food Safety Standard - Determination of Protein in Food (GB / T 5009.5-2003)" to detect inorganic nitrogen-containing substances and organic non-protein nitrogen-containing substances. Positive deficiency, by adding melamine, ammonium chloride, urea and other non-protein nitrogen to milk to increase the nitrogen content of the product, leading to milk adulteration incidents occur from time to time. Therefore, there is an urgent need for a method for detecting protein in milk to realize quantitative determination of protein content in milk. Contents of the invention [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 周焕英刘忠文高志贤王鸿英朱双双
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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