Analysis method of estriol
An analysis method, the technology of estriol, is applied in the direction of analyzing materials, material separation, measuring devices, etc., to achieve the effect of good separation and accuracy, high sensitivity and good separation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Solution preparation: Accurately weigh 20mg of crude estriol, put it in a 20ml measuring bottle, dilute with methanol and make up to volume, shake well.
[0026] Chromatographic conditions: hydrophilic octadecylsilane bonded silica gel column (5um, 4.6×250mm), mobile phase is acetonitrile-water, flow rate: 1.0ml / min, column temperature: 30°C, detection wavelength: 280nm, into The sample volume is 20ul.
[0027] Gradient elution: the volume ratio of acetonitrile to water: 0-10 minutes, 10-20: 90-80; 10-30 minutes, 60-80: 40-20; 30-55 minutes, 60-70: 40-30.
Embodiment 2
[0029] Solution preparation: Accurately weigh 20mg of crude estriol, put it in a 20ml measuring bottle, dilute with methanol and make up to volume, shake well.
[0030] Chromatographic conditions: hydrophilic octadecylsilane bonded silica gel column (5um, 4.6×250mm), mobile phase is acetonitrile-water, flow rate: 1.0ml / min, column temperature: 30°C, detection wavelength: 280nm, into The sample volume is 20ul.
[0031] Gradient elution: The volume ratio of acetonitrile to water is: 0-10 minutes, 30-40:70-60; 10-30 minutes, 20-40:80-60; 30-55 minutes, 80:20.
Embodiment 3
[0033] Solution preparation: Accurately weigh 20mg of crude estriol, put it in a 20ml measuring bottle, dilute with methanol and make up to volume, shake well.
[0034] Chromatographic conditions: hydrophilic octadecylsilane bonded silica gel column (5um, 4.6×250mm), mobile phase is acetonitrile-water, flow rate: 1.0ml / min, column temperature: 30°C, detection wavelength: 280nm, into The sample volume is 20ul.
[0035] Gradient elution: the volume ratio of acetonitrile to water: 0-10 minutes, 20-30: 80-70; 10-30 minutes, 40-60: 60-40; 30-55 minutes, 70-80: 30-20.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com