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Zinc ion detection method

A detection method and zinc ion technology, applied in the field of nano-fluorescent probes, can solve the problems of complicated probe synthesis steps and complex mechanism, and achieve the effects of good application prospect, good selectivity and simple method.

Active Publication Date: 2017-08-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Description
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  • Application Information

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Problems solved by technology

However, these probes generally have problems such as cumbersome synthesis steps and complex mechanisms.

Method used

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  • Zinc ion detection method
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  • Zinc ion detection method

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Experimental program
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Embodiment 1

[0028] This embodiment includes the following steps:

[0029] 1. Preparation of self-assembled nanoparticles: inject 50 μL of BODIPY small molecule solution with a concentration of 0.1 mM dissolved in DMF into 0.95 mL of HEPES buffer solution with a concentration of 5 mM and pH=7.4, and mix well to obtain self-assembled nanoparticles. Particles, spare.

[0030] 2. Draw a standard curve: take 150 μL of the solution obtained in step 1, and add 1.5 μL of zinc ion standard solutions of different concentrations, so that the concentrations of zinc ions in the mixed solution are 2, 5, 8, 10, 20, 30, 40, 50μM, after incubation for 10min, reserved for determination. Take another 150 μL of the solution obtained in step 1, and add 1.5 μL of deionized water as the blank control system solution. Place the standard solution and the blank control solution in a fluorescence cuvette with an optical path of 1 cm, scan the emission spectrum, and obtain the fluorescence spectrum, such as figur...

Embodiment 2

[0036] 1. Determination of zinc ions in human hair samples: collect human hair samples, wash them once with shampoo, wash them with deionized water for 10 times, then immerse them in acetone solution for 2 hours, then wash them with deionized water for 10 times. Dry in an oven at 80°C until constant weight, cut into hair sections less than 1cm with stainless steel scissors, and store in a desiccator. Weigh 9.84g of hair into a microwave digestion tank, add 8mL of 65% nitric acid and 1mL of 30% hydrogen peroxide, leave it at room temperature for 10h, install the digestion tank on the microwave digestion instrument, follow the program "130℃, 10min; 150℃ , 5min; 180°C, 40min" was digested at 480W power, the liquid obtained after digestion was transferred to a 50mL beaker, heated on an electric stove until the liquid just evaporated to dryness, and 1mL deionized water was added to the beaker. Shake in the container for 2 minutes, add 9 mL of HEPES buffer solution with a concentrat...

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Abstract

The invention relates to a zinc ion detection method based on self-assembly nano particles of BODIPY dye micromolecules. The BODIPY dye micromolecules gather in a water solution medium and are self-assembled to form nano particles, thus, fluorescence quenching is caused, fluorescence is recovered through addition of zinc ions, the fluorescence intensity change of a product obtained after mixing within a certain time period at the 600 nm wavelength and the concentration of added zinc ions are in direct proportion, and accordingly, quantitative determination of the zinc ions is realized. The method is high in sensitivity, good in selectivity, simple to operate and capable of being used for determining zinc ions in a human hair sample.

Description

technical field [0001] The invention relates to a nanometer fluorescent probe, in particular to a method for detecting zinc ions by utilizing self-assembled nanoparticles of BODIPY dye small molecules. Background technique [0002] Zinc is one of the most abundant trace elements in the human body. Most of it is combined with proteins, and as a cofactor or structural unit, it plays a vital role in cell division, immune regulation, signal transmission, gene transcription, etc. Severe zinc deficiency in humans is rare, but small or moderate zinc deficiency is common worldwide. Epidemiological studies have shown that zinc deficiency is closely related to the occurrence of diseases such as specific skin disorders, breast and ovarian cancer, and Alzheimer's disease. Therefore, it is of great significance to realize highly sensitive detection of zinc ions in human body. [0003] Traditional zinc ion detection methods include atomic absorption spectrometry, atomic emission spectro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31
Inventor 贾明艳冯亮李慧
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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