Method of measuring isocarbophos by fluorescent spectrometry of aptamermediating nitrogen-doped carbon spotscatalyzing H2O2 reaction with TMB

A technology of isocarbophos and H2O2 is applied in the field of analytical chemistry to achieve the effects of improved sensitivity, simple method and good selectivity

Inactive Publication Date: 2019-01-11
GUANGXI NORMAL UNIV
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Problems solved by technology

Based on the aptamer reaction between isocarbophos and aptamer, the fluorescence of nitrogen-doped carbon dots mediated by aptamer catalyzes H 2 o 2 Oxidation of TMB reacts to generate fluorescent products, and using this product as a fluorescent molecular probe to realize the determination of isocarbophos by fluorescence method has not been reported yet.

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  • Method of measuring isocarbophos by fluorescent spectrometry of aptamermediating nitrogen-doped carbon spotscatalyzing H2O2 reaction with TMB

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[0023] An aptamer-mediated nitrogen-doped carbon dot catalyzes H 2 o 2 The method for determining isocarbophos with TMB reaction fluorescence spectrometry may further comprise the steps:

[0024] (1) Prepare isocarbophos standard solution system with known concentration: Add 50 μL 0.1 mg / L carbon dot solution, 200 μL 1 mmol / L pH3.6 HAc-NaAc buffer solution, 30 μL 1.55 μmol / L isocarbophos aptamer solution (sequence is 5'-AGCTTGCTGCAGCGATTCTTGATCGCCACAGAGCT-3'), shake well; then add 2μL, 10μL, 30μL, 50μL, 70μL, 90μL, 120μL 15μg / L isocarbophos Standard solution, 40μL 2mmol / LH 2 o 2 Solution and 50μL 0.5mmol / L TMB solution, dilute to 1.5mL with twice distilled water, react in a water bath at 50°C for 15min, and cool to room temperature in ice water;

[0025] (2) Prepare blank control solution system: adopt the method of step (1) without adding isocarbophos standard solution to prepare blank control solution system;

[0026] (3) Get the isocarbophos standard solution system an...

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Abstract

The invention discloses a method of measuring isocarbophos by the fluorescent spectrometry of aptamer mediating nitrogen-doped carbon spots catalyzing H2O2 reaction with TMB. The method comprises thefollowing steps that (1), an isocarbophos standard solution system with preset concentration is prepared; (2), a blank comparison solution system is prepared; (3), delta F=F-F0 is calculated; (4), thedelta F is used for conducting a working curve for the concentration of the isocarbophos; (5), a tested sample solution is prepared, and delta F sample=F sample-F0 is calculated; and (6), the isocarbophos concentration in the tested sample is calculated. The catalysis of isocarbophos aptamer reacting mediating nitrogen-doped carbon spots (CD) is adopted, that is, the CD catalyzes the oxidation ofTMB by H2O2 to produce fluorescence products. The CD is combined with the catalysis, and therefore sensitivity is greatly improved. The fluorescence products are used as a fluorescence probe and arein linear relationship with the concentration of the tested isocarbophos. Quantitative measuring of the isocarbophos by nitrogen-doped carbon spots catalytic reacting fluorescent spectrometry is realized. The method is simple and quick, is excellent in selectivity and is high in sensitivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to an aptamer-mediated nitrogen-doped carbon point catalytic H 2 o 2 A method for the determination of isocarbophos by reaction fluorescence spectrometry with TMB. Background technique [0002] The research on the preparation method of carbon dots has always been one of the research hotspots in nanomaterials and analytical chemistry. At present, a series of carbon dot synthesis methods have been established, such as arc discharge method, laser etching method, chemical oxidation method, template method, microwave method and hydrothermal synthesis method, among which the microwave method is harmless because of its fast preparation speed. Water as a solvent and other advantages have attracted much attention. Existing literature reports that the prior art has the following preparation methods for carbon dots: a method of pyrolyzing glycerol with one-step microwave assistance in th...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/643G01N21/78
Inventor 李重宁李欣蒋治良梁爱惠
Owner GUANGXI NORMAL UNIV
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