Research method and tool for multi-target protein-DNA interaction

Abstract

The invention provides a targeting transposon complex with an oligonucleotide tag and application of the targeting transposon complex to research of interaction of multiple-target molecule-DNA.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a targeted transposome complex with an oligonucleotide label and its application for studying multi-target protein-DNA interaction. Background technique [0002] The regulation of gene expression is the basis for all life activities of living organisms. According to the central dogma of inheritance, biological genetic information is transcribed from DNA to form RNA, and then translated from RNA to form protein. Outside of classical genetics, when the nucleotide sequence of DNA does not change, the expression level of genes changes and can be stably passed on to offspring, which is called epigenetics. It determines when, where and how genes are expressed. Common epigenetic regulation includes DNA methylation, histone modification, chromatin conformation change, etc. [0003] The interaction between biomacromolecules is involved in regulating the selective expression of genes and the p...

AI Technical Summary

Problems solved by technology

[0008] The traditional ChIP-seq technology is affected by factors such as cross-linking / ultrasonic fragmentation conditions are not fixed, antibodies, etc., and requires a large number of cells / tissues to build a library, which is difficult to apply to micro-sample and single-cell experiments

[0009] ChIP-seq technology is prone to false negatives / false positives, due to the uneven interruption of ultrasound, which will lead to high sequencing background noise

[0010] Although CUT&Tag technology greatly shortens the experimental time compared with ChIP-seq technology, the steps are still cumbersome

[0011] Existing technologies are only suitable for research targeting a single target protein, and cannot simultaneously conduct multi-target research in the same sample

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