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Preparation method of deoxyribonucleic acid electrochemical nanometer sensor

A deoxyribonucleic acid and nano-sensor technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of failing to obtain sensitivity, and achieve the effect of firmly fixed quantity, improved detection sensitivity, and high sensitivity

Inactive Publication Date: 2004-09-01
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, good sensitivity was not obtained

Method used

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  • Preparation method of deoxyribonucleic acid electrochemical nanometer sensor
  • Preparation method of deoxyribonucleic acid electrochemical nanometer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The immobilization of a gold nanocrystal on the surface of a gold electrode:

[0023] 1). Immerse the cleaned gold electrode in a solution containing mercaptoethylamine for 1 hour at room temperature, take it out and clean it to clean the mercapto compound physically adsorbed on the surface of the electrode. Finally, rinse with a large amount of high-purity water and blow dry with high-purity nitrogen.

[0024] 2). Put the electrode into a gold nanocrystal solution with a pH value of 3, assemble it in the dark for 1 hour, and clean it with high-purity water after taking it out.

[0025] Immobilization of two target DNAs on gold nanocrystal modified electrodes:

[0026] Put the electrode modified with gold nanocrystals into the buffer solution containing the target DNA, pH 5. At 10°C, interact for 1 hour.

[0027] Three hybrids:

[0028] Put the gold electrode modified with the target DNA into the complementary DNA solution containing the silver nanocrystal label, ke...

Embodiment 2

[0032] The immobilization of a gold nanocrystal on the surface of a gold electrode:

[0033] 1). Immerse the treated and cleaned gold electrode in a solution containing mercaptoaniline for 10 hours at room temperature, take it out and clean it to clean the mercapto compound physically adsorbed on the surface of the electrode. Finally, rinse with a large amount of high-purity water and blow dry with high-purity nitrogen.

[0034] 2). Put the electrode into the gold nanocrystal solution with a pH value of 7, assemble it in the dark for 2 hours, and clean it with high-purity water after taking it out.

[0035] Immobilization of two target DNAs on gold nanocrystal modified electrodes:

[0036] Put the electrode modified with gold nanocrystals into the buffer solution containing the target DNA, pH 6. At 20°C, interact for 30 minutes.

[0037] Three hybrids:

[0038] Put the gold electrode modified with the target DNA into the complementary DNA solution containing the silver nan...

Embodiment 3

[0042] The immobilization of a gold nanocrystal on the surface of a gold electrode:

[0043] 1). Immerse the cleaned gold electrode in a solution containing mercaptoethylamine, soak it at room temperature for 4 hours, take it out and clean it, and clean the mercapto compound physically adsorbed on the surface of the electrode. Finally, rinse with a large amount of high-purity water and blow dry with high-purity nitrogen.

[0044] 2). Put the electrode into a gold nanocrystal solution with a pH value of 4, assemble it in the dark for 20 hours, and clean it with high-purity water after taking it out.

[0045] Immobilization of two target DNAs on gold nanocrystal modified electrodes:

[0046] Put the electrode modified with gold nanocrystals into the buffer solution containing the target DNA, pH 7. At 50°C, interact for 2 hours.

[0047] Three hybrids:

[0048] Put the gold electrode modified with the target DNA into the complementary DNA solution containing silver nanocrysta...

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Abstract

The invention belongs to a method of preparing deoxyribonucleic acid (DNA) electrochemical nano sensor. It fixes the nano gold crystal on the surface of the gold electrode, uses the property that nano gold crystal and mercapto functional group containing DNA can interact to fix sequence- known target single-string DNA (ssDNA) on the surface of the gold electrode. The nano silver crystal labeled compensatory DNA and the target ssDNA make cross reaction. In an acidic medium, oxidize and release the nano silver crystal to make the nano silver crystal exist in the solution in an ion state. It detects the quantity of silver ions by electrochemical method, thus detecting that of the target DNA. The sensor has very strong selectivity and very high sensitivity.

Description

technical field [0001] The invention belongs to the preparation method of deoxyribonucleic acid electrochemical nano sensor. Background technique [0002] The deoxyribonucleic acid (DNA) sequencing of the human genome has been initially completed, which will not only promote people's further understanding of the deadly disease that plagues mankind, but also help the development of diagnostics and therapeutics. For the detection of DNA sequences of specific pathogens or genetic diseases, most of the previous methods are based on the hybridization of probes labeled with radioactive, fluorescent and chemiluminescent substances to the target sequence. If used 32 P or 35 The S-labeled detection system is an early and commonly used detection method with high sensitivity, but this method requires specially trained technicians, and it also faces the problem of handling radioactively labeled active probes and its short storage time. longevity issue. Therefore, radiolabeled probes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N33/53G01N33/68
Inventor 李景虹王美佳刘晓庆
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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