17 results about "Recombinant Hirudin" patented technology

The invention relates to a recombinant hirudin fusion protein, a coding gene and a prokaryotic expression method and application of the recombinant hirudin fusion protein. The recombinant hirudin fusion protein comprises an HMg variant amino acid sequence and a fusion tag. The recombinant hirudin fusion protein has the advantages that the hirudin variant in poecilobdella manillensis is adopted to construct an efficient prokaryotic expression system, the hirudin yield and activity are improved under the condition that post-translational modification is not needed, the anticoagulant efficiency and the production process of the recombinant hirudin fusion protein provide an important basis for development of a new generation of antithrombotic drugs, and the recombinant hirudin fusion protein has wide application prospects. The progress of cardiovascular disease treatment and hirudin production is expected to be promoted.

This invention relates to the field of biomedicine, and particularly to a hirudin gene, expression vector, engineered bacteria, recombinant protein, and its applications and production methods, all possessing anticoagulant, thrombolytic, and antioxidant effects. The nucleotide sequence of the hirudin gene is shown in SEQ ID NO: 1. The amino acid sequence of the recombinant protein is shown in SEQ ID NO: 2. The recombinant protein of the hirudin gene provided by this invention, possessing anticoagulant, thrombolytic, and antioxidant effects, exhibits a high concentration of recombinant hirudin, significantly increasing the yield of recombinant hirudin. The recombinant hirudin also demonstrates strong in vitro anticoagulant and thrombolytic activity, indicating broad market prospects.

The invention belongs to the field of genetic engineering and synthetic biology, and particularly relates to tyrosine sulfation modified high-activity recombinant hirudin, saccharomyces cerevisiae engineering bacteria and application. According to the invention, saccharomyces cerevisiae is taken as a chassis cell, tyrosine protein sulfotransferase and related enzymes and transport systems are introduced through genetic engineering modification, efficient sulfation modification of the hirudin Tyr63 site is realized, and the anticoagulant activity of the hirudin Tyr63 site is remarkably improved. The method overcomes the limitations of high extraction cost of natural hirudin and lack of modification function in the existing recombination technology. In addition, the tyrosine sulfation modified high-activity recombinant hirudin provided by the invention shows an excellent antioxidant effect in the field of beauty and skin care, and experiments prove that the antioxidant activity of the tyrosine sulfation modified high-activity recombinant hirudin is equivalent to that of natural hirudin. The invention provides an innovative solution for low-cost and large-scale production of high-activity hirudin, and has the application potentials of medicines and cosmetics.

The invention belongs to the field of daily chemicals, and discloses a preparation method of a chlamydomonas reinhardtii source recombinant hirudin algae strain exosome with multiple skin care efficacy, which comprises the following steps: step 1, culturing a chlamydomonas reinhardtii source recombinant hirudin algae strain in a TAP culture medium to a logarithmic phase, and centrifuging to obtain a first supernatant; step 2, carrying out secondary centrifugation on the first supernatant to obtain a second supernatant; step 3, filtering the second supernatant, and performing third centrifugation to obtain a first precipitate; 4, washing the first precipitate, resuspending, and centrifuging for the fourth time to obtain a second precipitate; the second precipitate is the chlamydomonas reinhardtii source recombinant hirudin strain exosome. The chlamydomonas reinhardtii source recombinant hirudin strain exosome prepared by the preparation method has good oil control, anti-wrinkle, anti-aging, whitening, barrier repairing and soothing effects. Meanwhile, the invention further discloses application of the exosome and cosmetics.

The present application relates to the technical field of genetic engineering, in particular to a kind of tyrosine sulfated recombinant hirudin, preparation method and purposes thereof.In order to improve the safety of recombinant hirudin, synthesis yield, reduce production cost, the present application successfully prepares tyrosine sulfated recombinant hirudin by algal expression vector.

The invention belongs to the field of genetic engineering and synthetic biology, and particularly relates to tyrosine sulfation modified high-activity recombinant hirudin, saccharomyces cerevisiae engineering bacteria and application. According to the invention, saccharomyces cerevisiae is taken as a chassis cell, tyrosine protein sulfotransferase and related enzymes and transport systems are introduced through genetic engineering modification, efficient sulfation modification of the hirudin Tyr63 site is realized, and the anticoagulant activity of the hirudin Tyr63 site is remarkably improved. The method overcomes the limitations of high extraction cost of natural hirudin and lack of modification function in the existing recombination technology. In addition, the tyrosine sulfation modified high-activity recombinant hirudin provided by the invention shows an excellent antioxidant effect in the field of beauty and skin care, and experiments prove that the antioxidant activity of the tyrosine sulfation modified high-activity recombinant hirudin is equivalent to that of natural hirudin. The invention provides an innovative solution for low-cost and large-scale production of high-activity hirudin, and has the application potentials of medicines and cosmetics.

The invention relates to the technical field of biomedicine, in particular to a nano-composite hirudin protein line capable of remodeling glycolipid metabolism and a preparation method of the nano-composite hirudin protein line. The nano-composite hirudin protein line for remodeling glycolipid metabolism is prepared from the following components in parts by weight: 60 to 85 parts of type I collagen line, 5 to 10 parts of recombinant hirudin, 2 to 5 parts of liraglutide, 1 to 5 parts of ursodesoxycholic acid, 1 to 4 parts of polydopamine and 2 to 5 parts of N-hydroxysuccinimide-polyethylene glycol-maleimide. 2-5 parts of phenylboronic acid modified sodium alginate and 2-5 parts of gallic acid chitosan; according to the invention, on-demand accurate release of drugs in a pathological microenvironment is realized, the intervention efficiency is remarkably improved, the side effects of the system are reduced, meanwhile, a collaborative treatment network is formed, root intervention on complex metabolic disorders is realized, the product can actively remodel a local immune microenvironment while delivering the drugs, the process is controllable, the safety is excellent, and the application prospect is wide. And the benefit of traditional wire embedding weight reduction is greatly improved.

The present invention belongs to the field of genetic engineering and synthetic biology, and specifically relates to a tyrosine sulfated highly active recombinant hirudin, brewer's yeast engineered bacteria and applications. The present invention uses brewer's yeast as a chassis cell, and through genetic engineering modification, introduces tyrosine protein sulfonate transferase and related enzymes and transport systems to achieve efficient sulfate modification of the Tyr63 site of hirudin, significantly improving its anticoagulant activity. This method overcomes the limitations of the high cost of extracting natural hirudin and the lack of modification function of existing recombinant technologies. In addition, the tyrosine sulfated highly active recombinant hirudin provided by the present invention exhibits excellent antioxidant efficacy in the field of beauty and skin care, and experiments have shown that its antioxidant activity is equivalent to that of natural hirudin. The present invention provides an innovative solution for the low-cost, large-scale production of highly active hirudin, and has potential for application in both medicine and cosmetics.

The present invention relates to the technical field of genetic engineering, and in particular, relates to a method for constructing a recombinant hirudin expression vector using algae as chassis cells, and culture. The endogenous promoter beta2-tubulin and RBCS2 intron sequence constitute a key promoter, which is linked to a Hygromycin resistance gene; a 2A peptide self-cleavage sequence is added between a recombinant hirudin gene and the Hygromycin resistance gene, and an HA tag is connected at the terminus. Thus, an algal expression vector is constructed. In order to improve the safety and synthesis yield of the recombinant hirudin and reduce the production cost, an algal expression vector expressing the recombinant hirudin is successfully constructed. The expression vector can significantly improve the activity and yield of the recombinant hirudin.

The invention belongs to the field of daily chemicals, and discloses application of chlamydomonas reinhardtii recombinant hirudin in preparation of cosmetics with an oil control effect. The chlamydomonas reinhardtii recombinant hirudin has a good oil control effect, and can be used as an active ingredient in skin and scalp oil control products. Meanwhile, the invention further discloses a cosmetic containing the chlamydomonas reinhardtii recombinant hirudin.

The present invention belongs to the technical field of genetic engineering. Provided are a recombinant hirudin with tyrosine sulfation, a preparation method therefor and the use thereof. In order to improve the safety and synthesis yield of recombinant hirudin, and reduce the production cost, a recombinant hirudin with tyrosine sulfation is prepared by means of an algae expression vector.

The present invention relates to the field of fermentation production. Provided is a method for acquiring chlamydomonas reinhardtii source recombinant hirudin algae powder by means of a fermentation culture method. The method can effectively multiply the algae source recombinant hirudin.

The invention relates to a recombinant hirudin fusion protein, an intelligent response type anticoagulant material as well as a preparation method and application of the intelligent response type anticoagulant material. The intelligent response type anticoagulant material is formed by constructing the recombinant hirudin fusion protein with a carbohydrate binding domain (CBM) and combining the recombinant hirudin fusion protein with bacterial nanocellulose or a composite material of the bacterial nanocellulose. The recombinant hirudin fusion protein disclosed by the invention can specifically respond to blood coagulation formation related enzymes so as to recover the anticoagulant activity, and can be well combined with a bacterial nanocellulose material or a composite material thereof; the intelligent response type anticoagulant material is mild in preparation condition and green and environment-friendly in technology, and has great potential in the medical application aspect of membranous anticoagulant biological materials such as heart valves and the like and tubular anticoagulant biological materials such as artificial blood vessels and the like.

The invention relates to a reconstructed hirudin fusion protein and application thereof, and the reconstructed hirudin fusion protein comprises a first part of thrombus targeting peptide and a second part of hirudin or hirudin derivative mutant, wherein the first part of thrombus targeting peptide is connected with the second part of hirudin or hirudin derivative through a recognition sequence of thrombus site related enzyme. The anticoagulant is different from some traditional anticoagulants such as heparin and hirudin, and has important significance in preventing and treating thrombus and reducing side effects generated in prevention and treatment of thrombus.

This invention relates to a recombinant hirudin fusion protein with collagen affinity, its preparation method, and its applications. The fusion protein is a recombinant hirudin fusion protein incorporating a collagen-binding domain (CBD). The fusion protein of this invention possesses affinity for type I and type III collagen, and can inhibit thrombin activity to exert an anticoagulant effect. Furthermore, it can bind well to collagen-based composite materials, making it suitable for use in cardiovascular implants such as heart valves, cardiac occluders, dialysis membranes, oxygenation membranes, artificial blood vessels, and vascular stents, as well as in extracorporeal circulation and blood purification devices, enhancing anticoagulant and antithrombotic effects.

The invention belongs to the field of genetic engineering and synthetic biology, and particularly relates to tyrosine sulfation modified high-activity recombinant hirudin, saccharomyces cerevisiae engineering bacteria and application. According to the invention, saccharomyces cerevisiae is taken as a chassis cell, tyrosine protein sulfotransferase and related enzymes and transport systems are introduced through genetic engineering modification, efficient sulfation modification of the hirudin Tyr63 site is realized, and the anticoagulant activity of the hirudin Tyr63 site is remarkably improved. The method overcomes the limitations of high extraction cost of natural hirudin and lack of modification function in the existing recombination technology. In addition, the tyrosine sulfation modified high-activity recombinant hirudin provided by the invention shows an excellent antioxidant effect in the field of beauty and skin care, and experiments prove that the antioxidant activity of the tyrosine sulfation modified high-activity recombinant hirudin is equivalent to that of natural hirudin. The invention provides an innovative solution for low-cost and large-scale production of high-activity hirudin, and has the application potentials of medicines and cosmetics.

The invention relates to a preparation method of a magnetic navigation BMSC exosome loaded with recombinant hirudin, the preparation method comprises the following steps: firstly preparing IONP-BMSC-exos, then co-incubating the IONP-BMSC-exos and RH to obtain IONP-BMSC-exos-RH, the RH can be combined with PAR2 on the surface of the BMSC, and the loading capacity of the RH can be improved; magnetic particles are wrapped in the IONP-BMSC-exos-RH prepared by the preparation method disclosed by the invention, and the IONP-BMSC-exos-RH can be enriched in a magnetic region; the invention further relates to application of the IONP-BMSC-exos-RH in preparation of anticoagulant drugs, in-vitro experiments prove that the quality of thrombus of an IONP-BMSC-exos-RH group in 1.0-2.5 h is remarkably reduced, and it is indicated that the IONP-BMSC-exos-RH has an excellent anticoagulant effect; the invention further relates to application of the IONP-BMSC-exos-RH in preparation of the medicine for repairing the damaged bone tissue, in-vivo experiments prove that the IONP-BMSC-exos-RH can be enriched in an area near a hip joint where a neodymium magnet is implanted, thrombus formation in the femoral head is remarkably reduced, the good anticoagulant effect is achieved, and micro-CT detection shows that the IONP-BMSC-exos-RH can remarkably improve femoral head necrosis.