Recombinant hirudin fusion protein, intelligent response type anticoagulant material as well as preparation method and application of intelligent response type anticoagulant material

By combining CBM-mediated recombinant hirudin fusion protein with bacterial nanocellulose, a smart responsive anticoagulant material was constructed. This solved the problems of chemical inertness and weak interfacial binding force in the modification process of nanocellulose-based materials, achieving precise regulation and long-term anticoagulation, reducing safety risks, and improving the functional synergy and safety of the material.

CN121537527APending Publication Date: 2026-02-17DONGHUA UNIV
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Patent Information

Application Number
CN202511458121.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-13
Publication Date
2026-02-17

AI Technical Summary

Technical Problem

Existing nanocellulose-based anticoagulant materials suffer from limitations in chemical inertness, weak interfacial bonding, easy detachment of functional layers, and potential biosafety risks during the modification process. Furthermore, traditional anticoagulant drugs pose a bleeding risk, making it difficult to achieve precise control and long-term anticoagulation.

Method used

A recombinant hirudin fusion protein mediated by carbohydrate binding domain (CBM) was combined with bacterial nanocellulose and linked to a thrombin-associated enzyme recognition sequence via a flexible protein linker sequence to construct a smart responsive anticoagulant material. The protein was then fixed in a specific direction by utilizing the affinity of CBM for cellulose or chitosan. The recombinant protein was then expressed and purified in microbial strains using a 3A assembly toolkit.

Benefits of technology

This method achieves the addition of functional units without compromising the integrity of the nanonetwork, avoiding chemical crosslinking agent residues and coating instability. It possesses anticoagulant activity that precisely responds to the coagulation process, reduces the risk of systemic bleeding, and improves the safety and functional synergy of the material.

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Abstract

The invention relates to a recombinant hirudin fusion protein, an intelligent response type anticoagulant material as well as a preparation method and application of the intelligent response type anticoagulant material. The intelligent response type anticoagulant material is formed by constructing the recombinant hirudin fusion protein with a carbohydrate binding domain (CBM) and combining the recombinant hirudin fusion protein with bacterial nanocellulose or a composite material of the bacterial nanocellulose. The recombinant hirudin fusion protein disclosed by the invention can specifically respond to blood coagulation formation related enzymes so as to recover the anticoagulant activity, and can be well combined with a bacterial nanocellulose material or a composite material thereof; the intelligent response type anticoagulant material is mild in preparation condition and green and environment-friendly in technology, and has great potential in the medical application aspect of membranous anticoagulant biological materials such as heart valves and the like and tubular anticoagulant biological materials such as artificial blood vessels and the like.
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