Preparation method for sludge aerobic composting composite inoculum

A compound bacterial agent, aerobic composting technology, applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of unsatisfactory fertilizer effect, emission of odor from the heap, loss of organic matter, etc. Achieve good compost treatment effect, promote rapid decomposing, and reduce the effect of nitrogen loss

Active Publication Date: 2015-01-21
GUANGXI BOSSCO ENVIRONMENTAL PROTECTION TECH
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Problems solved by technology

[0003] For example, the Chinese Patent Application No. 201010514582.7 discloses "the method of aerobic composting of sludge by using composting compound bacterial agent", wherein the compound bacterial agent includes the same compound bacterial agent A and composite bacterial agent B, and the composite bacteria are inoculated at the initial stage of composting Agent A can promote the rapid decomposition of protein, fat, starch, etc. in the compost, so that the compost temperature can reach the fermentation high temperature period in advance, and inoculate a layer of composite strain B degradable fiber with a thickness of 0.1m on the surface of the compost in the form of covering during the high temperature period Hemicellulose and cellulose are used to achieve the composting effect, but this kind of inocul

Method used

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preparation example Construction

[0025] The preparation method of each bacterial strain in the described composite bacterial agent is as follows:

[0026] (1) Preparation of Bacillus subtilis bacterial liquid

[0027] Pick the Bacillus subtilis strain and inoculate it into the solid slant medium, place it in the incubator for cultivation, the cultivation temperature is 35-40°C, and the cultivation time is 18-24h. Then inoculate the grown slant colonies into the liquid medium for shaker culture, the shaker speed is 160r / min, the culture temperature is 35-40°C, and the culture time is 24-48 hours. When the number of colonies reaches 1×10 9 cuf / ml, the preparation of the bacterial solution is complete. The Bacillus subtilis solid slant culture medium is made by mixing the following raw materials by weight: 5g of glucose, 1g of beef extract, 10g of peptone, 5g of sodium chloride, 15g of agar, 1000ml of distilled water, and a pH value of 7.0; the liquid culture The base is solid slant medium formula de-agar. Bo...

Embodiment 1

[0038] Mix the bacterial liquid of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Candida utilis, and Phanerochaete chrysosporium in a volume ratio of 0.2:0.2:0.1:0.2:0.1, and inoculate it into the fermentation medium In this method, the amount of mixed bacterial solution is 12% of the weight of the fermentation medium, and it is cultured statically at 30°C for 12 days. When the fermentation medium is covered with hyphae, the total number of living microorganisms can reach 1×10 9 cuf / ml, culture complete. The formula of the fermentation medium is: brown sugar 15g, peptone 1g, bean cake powder 3g, potassium dihydrogen phosphate 0.1g, calcium chloride 0.1g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate 0.3 g, 0.5 g of sodium bicarbonate, 1000 ml of distilled water, and the pH value of the fermentation medium is 7.0. The fermentation medium was sterilized at 121°C for 30 minutes and cooled before use.

Embodiment 2

[0040] Mix the bacterial liquid of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Candida utilis, and Phanerochaete chrysosporium in a volume ratio of 0.4:0.3:0.1:0.4:0.4, and inoculate it into the fermentation medium , the amount of mixed bacterial solution is 10% of the weight of the fermentation medium, and it is cultured statically at 35°C for 10 days. 9 cuf / ml, culture complete. The formula of the fermentation medium is: brown sugar 20g, peptone 1.5g, bean cake powder 2g, potassium dihydrogen phosphate 0.2g, calcium chloride 0.2g, ammonium sulfate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g, acetic acid Sodium 0.5g, sodium bicarbonate 1g, distilled water 1000ml, fermentation medium pH value 7.0. The fermentation medium was sterilized at 121°C for 30 minutes and cooled before use.

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Abstract

The invention discloses a preparation method for a sludge aerobic composting composite inoculums. According to the method, the Bacillus subtilis (No. 10066), the Bacillus licheniformis (No. 10103), the Lactobacillus acidophilus (No. 6075), the Candida utilis (No. 31272), and the Phanerochaete chrysosporium (No. 40719) that are preserved in China Center of Industrial Culture Collection (CICC) are subjected to individual culture, and are then mixed in proportion and inoculated into a fermentation culture to perform expanded culture, thus obtaining the composite inoculum. At the initial stage of inoculating the composite inoculums to sludge aerobic composting, the time for the compost body to reach a high temperature period is advanced by 4-6 days, the compositing period is shortened by 11-14 days, the stack moisture content is decreased by 18%-25%, and the total nitrogen content of the compost body is increased by 15%-20%, thus improving the composting effect.

Description

technical field [0001] The invention relates to a sludge aerobic composting compound bacterial agent, in particular to a preparation method of the sludge aerobic composting compound bacterial agent. Background technique [0002] With the acceleration of my country's urbanization process, the amount of urban sewage treatment continues to increase, and the sewage treatment technology has become increasingly mature. The accompanying problem is how to deal with the large amount of excess sludge generated when the sewage treatment plant treats sewage. At present, the treatment and disposal methods of surplus sludge in my country mainly include dry incineration, sanitary landfill, composting and so on. Among them, the sludge composting technology is usually mixed with municipal garbage, livestock and poultry manure, straw, wood chips, fly ash and other auxiliary materials for aerobic composting, which is an active and effective sludge resource and harmless treatment and disposal m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F17/00C12R1/125C12R1/10C12R1/23C12R1/72C12R1/645
CPCC05F11/08C12N1/14C12N1/16C12N1/20Y02W30/40
Inventor 周永信张健宋海农徐国涛朱琦夏兴良罗剑云苏丽梅
Owner GUANGXI BOSSCO ENVIRONMENTAL PROTECTION TECH
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