Preparation method for sludge aerobic composting composite inoculum
A compound bacterial agent, aerobic composting technology, applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of unsatisfactory fertilizer effect, emission of odor from the heap, loss of organic matter, etc. Achieve good compost treatment effect, promote rapid decomposing, and reduce the effect of nitrogen loss
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preparation example Construction
[0025] The preparation method of each bacterial strain in the described composite bacterial agent is as follows:
[0026] (1) Preparation of Bacillus subtilis bacterial liquid
[0027] Pick the Bacillus subtilis strain and inoculate it into the solid slant medium, place it in the incubator for cultivation, the cultivation temperature is 35-40°C, and the cultivation time is 18-24h. Then inoculate the grown slant colonies into the liquid medium for shaker culture, the shaker speed is 160r / min, the culture temperature is 35-40°C, and the culture time is 24-48 hours. When the number of colonies reaches 1×10 9 cuf / ml, the preparation of the bacterial solution is complete. The Bacillus subtilis solid slant culture medium is made by mixing the following raw materials by weight: 5g of glucose, 1g of beef extract, 10g of peptone, 5g of sodium chloride, 15g of agar, 1000ml of distilled water, and a pH value of 7.0; the liquid culture The base is solid slant medium formula de-agar. Bo...
Embodiment 1
[0038] Mix the bacterial liquid of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Candida utilis, and Phanerochaete chrysosporium in a volume ratio of 0.2:0.2:0.1:0.2:0.1, and inoculate it into the fermentation medium In this method, the amount of mixed bacterial solution is 12% of the weight of the fermentation medium, and it is cultured statically at 30°C for 12 days. When the fermentation medium is covered with hyphae, the total number of living microorganisms can reach 1×10 9 cuf / ml, culture complete. The formula of the fermentation medium is: brown sugar 15g, peptone 1g, bean cake powder 3g, potassium dihydrogen phosphate 0.1g, calcium chloride 0.1g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, ferrous sulfate 0.01g, sodium acetate 0.3 g, 0.5 g of sodium bicarbonate, 1000 ml of distilled water, and the pH value of the fermentation medium is 7.0. The fermentation medium was sterilized at 121°C for 30 minutes and cooled before use.
Embodiment 2
[0040] Mix the bacterial liquid of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Candida utilis, and Phanerochaete chrysosporium in a volume ratio of 0.4:0.3:0.1:0.4:0.4, and inoculate it into the fermentation medium , the amount of mixed bacterial solution is 10% of the weight of the fermentation medium, and it is cultured statically at 35°C for 10 days. 9 cuf / ml, culture complete. The formula of the fermentation medium is: brown sugar 20g, peptone 1.5g, bean cake powder 2g, potassium dihydrogen phosphate 0.2g, calcium chloride 0.2g, ammonium sulfate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.02g, acetic acid Sodium 0.5g, sodium bicarbonate 1g, distilled water 1000ml, fermentation medium pH value 7.0. The fermentation medium was sterilized at 121°C for 30 minutes and cooled before use.
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