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Technology for degrading anthraquinone compounds in wastewater by use of phanerochaete chrysosporium

A technology of Phanerochaete and process technology, which is applied in the direction of fungi, water pollutants, microorganisms, etc., and can solve problems not involved in process technology

Inactive Publication Date: 2014-01-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some other doctoral and master's dissertations reported that Phanerochaete chrysosporium adsorbed and degraded the mechanism of anthraquinone compounds, but did not involve its process technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of cultures on test tube slant

[0023] A ring of Phanerochaete chrysosporium strains ( Phanerochaete chrysosporium ) (CCGMC5.776), 26°C, cultured for 50 hours; stored at 4°C for later use.

[0024] 2. Preparation of cultured strains in liquid shake flasks

[0025] Accurately weigh 5 grams of glucose, 0 grams of peptone, 5 grams of yeast extract, 0.2 grams of magnesium sulfate, 0.5 grams of Tween 80, 2 grams of potassium dihydrogen phosphate, 1000 mL of water, pH 5, and pack into 250 mL Erlenmeyer flasks, each containing 50 grams, 20 bottles in total, sterilized at 120°C for 40 minutes; ６ spores), cultured at 25°C, 150 rpm, for 72 hours;

[0026] 3. Preparation of primary liquid culture

[0027] Accurately weigh 50 grams of glucose, 0 grams of peptone, 50 grams of yeast extract, 2 grams of magnesium sulfate, 5 grams of Tween 80, 20 grams of potassium dihydrogen phosphate, and 10 L of water. Minutes; after sterilization and cooling, at a temperature o...

Embodiment 2

[0031] 1. Preparation of cultures on test tube slant

[0032] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technical Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Agricultural Microbiological Strain Preservation Management Center ACCC 30942), 30°C, incubation time 100 hours; store at 7°C for later use.

[0033] 2. Preparation of cultured strains in liquid shake flasks

[0034]Accurately weigh 150 grams of glucose, 25 grams of peptone, 25 grams of yeast extract, 0.4 grams of magnesium sulfate, 4 grams of Tween 80, 5 grams of potassium dihydrogen phosphate, 10 L of water, pH 6, and pack into 250 mL Erlenmeyer flasks, each 100 grams, 100 bottles in total, sterilized at 130°C for 30 minutes; ６ spores), cultured at 30°C, 150 rpm, for 50 hours;

[0035] 3. Preparation of primary liquid culture

[0036] Accurately weigh 2000 grams of glucose, 25 grams of pepto...

Embodiment 3

[0041] 1. Preparation of cultures on test tube slant

[0042] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Industrial Microbiology Strain Preservation Management Center CICC14067), 35°C, incubation time 144 hours; store at 10°C for later use.

[0043] 2. Preparation of cultured strains in liquid shake flasks

[0044] Accurately weigh 300 grams of glucose, 50 grams of peptone, 0 grams of yeast extract, 10 grams of magnesium sulfate, 100 grams of Tween 80, 90 grams of potassium dihydrogen phosphate, 10 L of water, pH 7.5, and pack in 250 mL triangular flasks, each with 120 mL, a total of 80 bottles, sterilized at 140°C for 20 minutes; ６ spores), cultured at 35°C, 150 rpm, for 72 hours;

[0045] 3. Preparation of primary liquid culture

[0046] Accurately weigh 1500 grams of glucose, 250 grams of ...

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PUM

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Abstract

The invention discloses a technology for degrading anthraquinone compounds in wastewater by use of phanerochaete chrysosporium, and relates to the field of bioengineering. The phanerochaete chrysosporium is used as an original strain; after liquid shake-flask culture and liquid strain enlarge culture, the bacterial liquid is transferred to the wastewater containing anthraquinone compounds, and certain nutrient substances are supplemented to perform fermentation culture. By adopting the technology, the removal rate of anthraquinone compounds can reach 60-100%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to using Phanerochaete chrysosporium to degrade wastewater containing anthraquinone compounds. After concentrating or diluting the raw waste water until the content of the anthraquinone compounds is 1-50 g / L, the components of the culture medium are supplemented, and Phanerochaete chrysosporium strains are introduced to degrade the anthraquinone compounds in the waste water. After degradation by Phanerochaete chrysosporium, the removal rate of anthraquinone compounds can reach 60-100%. [0002] Background technique [0003] There are many kinds of anthraquinone compounds, which are important chemical raw materials and intermediates, with bright colors and wide range, so they are widely used in textile, printing and dyeing industries, not only for printing and dyeing of polyester and blended fabrics, but also for nylon, acrylic, polypropylene , Acetate, wool, silk printing a...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/14C12R1/645C02F101/34
Inventor 张志才
Owner JIANGSU UNIV
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