Method for removing heavy metal pollutant from water body by using phanerochete chrysosporium

A technology of Phanerochaete chrysosporium and Phanerochaete, which is applied in the field of wastewater treatment and microbial application, can solve the problems of harsh reaction conditions, poor recycling effect, and unsatisfactory treatment effect, so as to reduce costs and avoid high energy consumption problems Effect

Inactive Publication Date: 2011-06-22
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of Phanerochaete chrysosporium adsorption reaction to treat wastewater generally has the problem of harsh reaction conditions, which is the main obstacle for the large-scale practical application of Phanerochaete chrysosporium to adsorb heavy metals.
[0003] For example, at present, the use of Phanerochaete chrysosporium to treat heavy metal wastewater uses more static adsorption methods, adding dried or modified Phanerochaete chrysosporium (mostly dead bacteria that have lost their activity) to heavy metal wastewater, increasing The treatment cost is reduced, and the reuse effect is poor; when the wastewater composition is complex, the treatment effect is not ideal, which is also a problem that needs to be solved in practical application

Method used

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  • Method for removing heavy metal pollutant from water body by using phanerochete chrysosporium

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Embodiment 1

[0022] A method of utilizing Phanerochaete chrysosporium to remove heavy metal cadmium in waste water of the present invention comprises the following steps:

[0023] (1) Growth stage: Suspend the spore powder of Phanerochaete chrysosporium BKM-F1767 (the deposit number in the American Type Culture Collection is ATCC 24725, preferably but not limited to this strain) in sterile water Spore suspension, then inoculate the spore suspension into Kirk liquid medium (preferably using the medium, but not limited to this), shake and cultivate at 37°C for 72h, control the rotation speed at 160rpm during cultivation, and obtain the following after filtration: figure 1 Shown Phanerochaete chrysosporium bacterial ball, wherein, the main component of Kirk liquid culture medium is: 0.2g / L KH 2 o 4 , 0.05g / L MgSO 4 ·7H 2 O, 0.01g / L CaCl 2 , 1mL / L inorganic solution, 0.5mL / L vitamin solution, 1.2mmol / L ammonium tartrate, 1% (mass fraction) glucose, 20mmol / L sodium acetate.

[0024] (2) A...

Embodiment 2

[0040] A method of utilizing Phanerochaete chrysosporium to remove heavy metal copper in waste water of the present invention comprises the following steps:

[0041] (1) Growth stage: this step is the same as Step 1 of Example 1;

[0042] (2) Adsorption stage: Add the cultured Phanerochaete chrysosporium bacteria balls to the copper-containing wastewater, the addition amount is 5g / 100mL wastewater, the concentration of copper-containing wastewater is 80mg / L, and the pH value of the wastewater is adjusted to 6.5, carry out the adsorption reaction at a constant temperature of 25°C, the speed of the reaction is controlled at 140rpm, the oscillation reaction is 6h, and the adsorption of copper in the wastewater is completed. After the reaction, the wastewater is filtered to remove the bacteria balls, and the bacteria balls of Phanerochaete chrysosporium are used to complete the reaction. One removal process.

[0043] Among them, the selection of the optimal copper-containing wast...

Embodiment 3

[0058] A method of utilizing Phanerochaete chrysosporium to remove heavy metal cadmium and copper in waste water of the present invention comprises the following steps:

[0059] (1) Growth stage: this step is the same as Step 1 of Example 1;

[0060] (2) Adsorption stage: use NaOH (or HNO 3 ) to adjust the pH value of the wastewater containing the culture solution (50% of the culture solution) with an initial copper concentration of 20 mg / L and an initial cadmium concentration of 20 mg / L respectively, so that the pH is 6.5, and then put into culture Phanerochaete chrysosporium bacteria balls (wet weight 5g / 100mL wastewater), at a temperature of 25°C and a shaker speed of 140rpm, shake the reaction, filter the wastewater after the reaction, recover the bacteria balls, and use Phenoriochaete chrysosporium flat leather Bacteria balls complete a removal process;

[0061] The concentrations of cadmium and copper in the treated wastewater samples were measured at 0.5h, 1h, 2h, 3h,...

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Abstract

The invention discloses a method for removing a heavy metal pollutant from a water body by using phanerochete chrysosporium. The method comprises the following steps of: preparing spore suspension by using phanerochete chrysosporium spore powder; inoculating into a liquid culture medium for shake culture; filtering to obtain phanerochete chrysosporium pellets; adding the pellets into wastewater, adjusting the pH value of the wastewater, performing adsorption reaction under a constant temperature condition, filtering the wastewater after the reaction, and recovering the pellets to finish a removal process one time; cleaning the recovered pellets by using deionized water and then adding the cleaned pellets into culture solution for continuous culture so as to obtain phanerochete chrysosporium regenerative pellets; and adding the phanerochete chrysosporium regenerative pellets into heavy metal wastewater to be treated for cycling. By the method, hyphae are not required to be modified and pretreated and can be repeatedly used, the reaction condition is simple, and the water body of a complex composition can be treated.

Description

technical field [0001] The invention relates to the field of microorganism application and waste water treatment, in particular to the application of Phanerochaete chrysosporium in treating heavy metal waste water. Background technique [0002] At present, in the field of wastewater treatment, Phanerochaete chrysosporium is used to treat heavy metal wastewater, which has attracted the attention of scholars at home and abroad in recent years. The existing technology uses Phanerochaete chrysosporium to remove heavy metals in wastewater mainly by biosorption. The biosorption method has the advantages of high removal efficiency, fast speed, low cost, and no secondary pollution. However, the use of Phanerochaete chrysosporium adsorption reaction to treat wastewater generally has the problem of harsh reaction conditions, which is the main obstacle for the large-scale practical application of Phanerochaete chrysosporium to adsorb heavy metals. [0003] For example, at present, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F1/28C02F1/62C02F101/20
Inventor 陈桂秋范佳琦曾光明陈安伟邹正军张文娟王亮
Owner HUNAN UNIV
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