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Specific bacterial strain for fusing two fungi and a protoplast and constructing method thereof

A technology of protoplasts and construction methods, which is applied in the fields of biochemical equipment and methods, chemical instruments and methods, and botanical equipment and methods. , the effect of high adaptability

Inactive Publication Date: 2003-03-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no three kinds of microbial protoplast fusion strains and construction patents dedicated to wastewater treatment, especially the construction strain technology for protoplast fusion of eukaryotic and prokaryotic strains

Method used

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  • Specific bacterial strain for fusing two fungi and a protoplast and constructing method thereof
  • Specific bacterial strain for fusing two fungi and a protoplast and constructing method thereof
  • Specific bacterial strain for fusing two fungi and a protoplast and constructing method thereof

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Embodiment Construction

[0016] In the present invention, the protoplasts of white rot fungi and parent strain 2 native bacteria YZ1 cells are generally fused first and then fused with Saccharomyces cerevisiae cells is better, that is: white rot fungi+native bacteria YZ1=Fhh (first fusion), Fhh+Saccharomyces cerevisiae Cells = Fhhh (second fusion). The following are examples: A. Reagent source: Helicase and lysozyme are products of Huamei Company in Shanghai; Polyethylene glycol (MW=6000) is imported from Japan; Giemsa stain is a product of Sigma Company in the United States; all the other reagents are domestic analytical grade Chemical reagent B. Basic liquid medium (MM): 1000ml: K 2 HPO 4 3g; KH 2 PO 4 1g; NH 4 NO 3 0.5g; Na 2 SO 4 0.1g; MgSO 4 ·7H 2 O 10mg; MnSO 4 4H 2 O 1 mg; CaCl 2 0.5mg; FeSO 4 ·7H 2 O 1 mg; CH 3 5g of COONa; 5g of yeast extract; 10g of peptone; 10g of glucose; 200g of potato extract, adjust the pH to 7.0; Optimal culture temperature: 35°C; optimal pH: 7.0....

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Abstract

The invention refers to a specific bacterium plant fused by the protoplast of two epiphytes and one bacterium and its structuring method which fuses the protoplast of true nucleus cell of white rotten epiphyte (phanerochaete chrysosporium), original nucleus cell (Pseudomonas) of aboriginal bacterium YZ1 and real nucleus cell of vintage yeast (Saccharomyces cerevisiae), and by three relative genes' reformation and conformity in a same cell, structures the gene-engineering specific bacterium. The technique consists of parent and three-parent transboundary fusion.

Description

1. Technical field: [0001] The present invention relates to a special-effect bacterial strain and its construction method, especially a kind of biological construction and application technology of a new microbial strain for degrading organic pollutants, more specifically, a special-effect bacterial strain in which two fungi are fused with a bacterial protoplast and its construction method. 2. Background technology: [0002] As far as protoplast fusion biotechnology is concerned, there are various protoplast fusion patents in the world, but there are no patents on the fusion technology of triparental strains from the two kingdoms. Such as strains used to select antibiotics, such as Imada C, Isolation and characterization of the interspecific fusants from Streptomycetes obtained using aliquor regeneration method, FISHERIES SCI, 68(2): 395-402, 2002; and Baltz RH, Genetic methods and strategies for Secondary metabolite yield improvement inantinomycetes, ANTON TEEUW INT J G 79...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N15/08
Inventor 程树培郝春博阙子龙陈俊顾继东李维新黄秋明于红霞孙成严俊张徐祥石磊
Owner NANJING UNIV
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