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Compound bacterial agent applied to high-efficiency quick straw decomposition and application of compound bacterial agent

A technology of compound bacterial agent and straw, which is applied in the field of comprehensive utilization of crop straw, to achieve the effect of reducing the use of chemical fertilizers

Inactive Publication Date: 2015-01-21
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to overcome the shortcomings of traditional straw decomposing agents, and provide a compound bacterial agent for straw decomposing with high efficiency for comprehensive utilization of straw

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of dewaxing bacteria agent:

[0026] According to the number of effective bacteria, the ratio of Bacillus cereus (Bacillus cereus): Bacillus subtilis (Bacillus subtilis): Clostridium pasteurianum (Clostridium pasteurianum) is 35%: 35%: 30%, and the three are added to the general sporulation culture Base. The pH of the culture medium is 6.8, the culture temperature is 20°C, the stirring speed is 250 rpm, and the sterile ventilation volume is 0.5m 3 / 1000L culture medium, the culture time is 1-3 days, after the culture, the spores and bacteria are collected by centrifugation and filter with adsorbent plate and frame, air-dried, spray-dried or freeze-dried below 80°C, and then crushed into powder. Obtain the dewax fungicide preparation.

[0027] 2. Preparation of antibacterial agents

[0028] Add white rot fungus (P.chrysosporium), Aspergilus oryzae, and Trichoderma viride to the container at a ratio of 25%: 25%: 50% based on the number of effective bacte...

Embodiment 2

[0036] 1. Preparation of dewaxing bacteria agent:

[0037] According to the number of effective bacteria, the ratio of Bacillus cereus (Bacillus cereus): Bacillus subtilis (Bacillus subtilis): Clostridium pasteurianum (Clostridium pasteurianum) is 36%: 36%: 28%, and the three are added to the general sporulation culture Base. The pH of the medium is 6.9, the culture temperature is 25°C, the stirring speed is 260 rpm, and the sterile ventilation volume is 0.5m 3 / 1000L culture medium, the culture time is 1-3 days, after the culture, collect the spores and bacteria by centrifugation and filter with adsorbent plate and frame, use air-drying, spray-drying or freeze-drying below 80°C, and then break into powder , to obtain the dewaxing agent preparation. .

[0038] 2. Preparation of antibacterial agents

[0039] Add white rot fungus (P.chrysosporium), Aspergilus oryzae, and Trichoderma viride to the container at a ratio of 26%: 26%: 48% according to the effective number...

Embodiment 3

[0048] 1. Preparation of dewaxing bacteria agent:

[0049] According to the number of effective bacteria, the ratio of Bacillus cereus (Bacillus cereus): Bacillus subtilis (Bacillus subtilis): Clostridium pasteurianum (Clostridium pasteurianum) is 37%: 37%: 26%, and the three are added to the general sporulation culture Base. The pH of the medium is 7.0, the culture temperature is 30°C, the stirring speed is 270 rpm, and the sterile ventilation volume is 0.7 m 3 / 1000L culture medium, the culture time is 1-3 days, after the culture, collect the spores and bacteria by centrifugation and filter with adsorbent plate and frame, use air-drying, spray-drying or freeze-drying below 80°C, and then break into powder , to prepare the dewaxing agent preparation.

[0050] 2. Preparation of antibacterial agents

[0051] Add white rot fungus (P.chrysosporium), Aspergilus oryzae, and Trichoderma viride to the container at a ratio of 28%: 28%: 44% based on the number of effective b...

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Abstract

The invention belongs to the field of comprehensive utilization of crop straws and relates to a compound bacterial agent applied to high-efficiency quick straw decomposition. The compound bacterial agent applied to the high-efficiency quick straw decomposition is composed of a dewaxing bacterial agent and an enamelysin bacterial agent, wherein the dewaxing bacterial agent is a compound bacterial agent of Bacilluscereus, Bacillussubtilis and Clostridiumpasteurianum, and the enamelysin bacterial agent is a compound bacterial agent of P.chrysosporium, Aspergilusoryzae and Trichodermaviride. The compound bacterial agent applied to the high-efficiency quick straw decomposition can realize high-efficiency quick straw decomposition, straws are converted into an environmental-friendly organic fertilizer, and cyclic regeneration of biological energy sources is realized.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of crop stalks, and relates to a compound bacterial agent applied to efficient and fast rot of straws and its application in high-efficiency and fast rot of straws. Background technique [0002] In the context of depletion of available resources and increasingly severe environmental pollution, my country, as the country with the largest population base in the world, faces a more serious and complex energy strategy situation. At the same time, China is also a large agricultural country, and the annual crop straw residues (such as wheat straw, rice straw, sorghum stalks, corn stalks, hemp stalks, cotton stalks, etc.) reach 1.2 billion tons. Since my country's main crops are mainly wheat and rice, accounting for about 70% of the total straw, this is a huge renewable resource. However, random discarding and uncontrolled burning have not only caused serious environmental pollution but also a ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/085C12R1/125C12R1/145C12R1/645C12R1/69C12R1/885
CPCC05F11/00C05F11/08C12N1/14C12N1/20C05F17/00
Inventor 曾德芳李壮杜友福李华峰刘常清王晨阳王飞宇田小凤
Owner WUHAN UNIV OF TECH
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