Compound microbial preparation as well as preparation method and application thereof

A technology of compound microorganisms and preparations, applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc., can solve the problems of long adsorption time and weak tolerance, and achieve high adsorption efficiency, easy control and practicality strong effect

Inactive Publication Date: 2015-01-28
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a single adsorbent can no longer meet the needs of composite wastewater, so it is necessary to study multifunctional composite microbial preparations to achieve the purpose of efficiently treating composite wastewater by combining the advantages of multiple materials
[0004] Phanerochaete chrysosporium is a kind of white rot fungus, which has a strong adsorption effect on heavy metals, but the adsorption time of Phanerochaete chrysosporium is longer and the tolerance is weaker in the composite wastewater with higher concentration

Method used

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  • Compound microbial preparation as well as preparation method and application thereof
  • Compound microbial preparation as well as preparation method and application thereof
  • Compound microbial preparation as well as preparation method and application thereof

Examples

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Embodiment 1

[0039] A compound microbial preparation, comprising Phanerochaete chrysosporium bacteria balls, polyvinyl alcohol, silicon dioxide, active carbon, sodium alginate and zeolite powder. Mix Phanerochaete chrysosporium balls, polyvinyl alcohol, silicon dioxide, activated carbon, sodium alginate and zeolite powder to form a mixed solution, and add it dropwise to sodium dihydrogen phosphate solution to form a solution with a diameter of 2cm to 4cm and a water content of 84% ± 0.5% microspheres (ie composite microbial preparations).

[0040] In the above compound microbial preparation, polyvinyl alcohol and silicon dioxide are loaded on the Phanerochaete chrysosporium ball; sodium alginate, zeolite powder and activated carbon are wound on the hyphae of the Phanerochaete chrysosporium ball.

[0041]A kind of preparation method of the composite microbial preparation of above-mentioned present embodiment, comprises the following steps:

[0042] (1) Preparation of gel solution by heatin...

Embodiment 2

[0053] A compound microbial preparation, comprising Phanerochaete chrysosporium bacteria balls, polyvinyl alcohol, silicon dioxide, active carbon, sodium alginate and zeolite powder. Mix Phanerochaete chrysosporium balls, polyvinyl alcohol, silicon dioxide, activated carbon, sodium alginate and zeolite powder to form a mixed solution, and add it dropwise to sodium dihydrogen phosphate solution to form a solution with a diameter of 2cm to 4cm and a water content of 86% ± 0.5% microspheres (ie composite microbial preparations).

[0054] In the above compound microbial preparation, polyvinyl alcohol and silicon dioxide are loaded on the Phanerochaete chrysosporium ball; sodium alginate, zeolite powder and activated carbon are wound on the hyphae of the Phanerochaete chrysosporium ball.

[0055] A kind of preparation method of the composite microbial preparation of above-mentioned present embodiment, comprises the following steps:

[0056] (1) Preparation of gel solution by heati...

Embodiment 3

[0063] A kind of composite microbial preparation of embodiment 1 is in the application of cadmium and 2,4-dichlorophenol (2,4-DCP) in adsorption degradation waste water, and concrete application method comprises the following steps:

[0064] (1) Get 2,4-dichlorophenol initial concentration is 40mg / L, cadmium initial concentration is respectively 5mg / L, 10mg / L, 20mg / L, 40mg / L, 80mg / L, 120mg / L, 160mg / L L, each 200ml of 8 groups of waste water of 200mg / L, adjust pH to be 6.5.

[0065] (2) The compound microbial preparation of Example 1 was added to the above-mentioned 8 groups of waste water in an amount of 4.1 g / L, and cultured by shaking at 37°C and 150 rpm for 96 hours, and then separated into solid phase and liquid phase after standing , measure cadmium and 2,4-dichlorophenol (2,4-DCP) residues in each group of waste water samples, and the measurement results are shown in Table 2 and image 3 . image 3 It is a schematic diagram of the adsorption effect of the composite mic...

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Abstract

The invention discloses a compound microbial preparation as well as a preparation method and application thereof. The preparation comprises phanerochaete chrysosporium balls, polyvinyl alcohol, silicon dioxide, activated carbon, sodium alginate and zeolite powder, which form the compound microbial preparation through crosslinking by a solution with sodium dihydrogen phosphate. The preparation method comprises the steps of preparing a gel liquid with the polyvinyl alcohol, sodium alginate, silicon dioxide and zeolite powder, mixing with a phanerochaete chrysosporium spore suspension with the activated carbon, then gradually dripping into the solution with the sodium dihydrogen phosphate so as to prepare embedded balls, and cultivating by a Kirk inorganic culture solution so as to prepare the compound microbial preparation. The compound microbial preparation has many gaps, high adsorption efficiency and good selectivity, the adsorbent has strong stability, the preparation method has the advantages of simple operation, low cost, affordability and so on, and the compound microbial preparation can be used for eliminating cadmium and 2, 4-dichlorophen in waste water.

Description

technical field [0001] The invention relates to the field of microbial preparations and wastewater treatment, in particular to a composite microbial preparation, a preparation method of the composite microbial preparation and an application in removing heavy metals and organic pollutants in wastewater. Background technique [0002] Due to the rapid increase of population, rapid industrialization and urbanization, the pollution of complex wastewater polluted by heavy metals and organic matter is getting worse day by day. In recent years, complex wastewater containing both heavy metals and organic matter has received more and more attention. Before these water bodies are discharged into receiving water bodies, they must be treated to reduce their concentration to the discharge concentration allowed by laws and regulations. Therefore, the treatment of compound wastewater has become a major problem that needs to be solved urgently at this stage. [0003] The biological adsorpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/08C12N11/04C02F3/34C02F101/22C02F101/36
Inventor 黄真真陈桂秋曾光明陈安伟郭志左亚男谭琼宋忠贤
Owner HUNAN UNIV
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