The invention relates to the technical field of extruding and granulating and especially relates to a granulating chamber of a biological particle fuel extruding machine. The granulating camber comprises a granulating chamber body, a rotating shaft, a stirring and extruding blade, a cylinder mould and a power device, wherein the cylinder mould is arranged in the granulating chamber body, a feedingpipe and a discharging opening are arranged on the granulating chamber body, the rotating shaft is arranged in the cylinder mould, the stirring and extruding blade is arranged in the peripheral direction of the rotating shaft, a cavity is formed in a mould wall of the cylinder mould, an air outlet is formed in an outer wall of the cylinder mould, a plurality of granulating pipes are arranged on the mould wall of the cylinder mould, an air blowing device is arranged in the granulating chamber body, a swinging plate is hinged to an inner wall of the cylinder mould, the stirring and extruding blade can extrude the swinging plate intermittently, an elastic cover is arranged between the inner wall, close to the swinging plate, of the cylinder mould and the swinging plate, a push portion is connected to the swinging plate, and the upper end of the push portion is arranged in the elastic cover. By means of the scheme, heat generated by the walls of the cylinder mould is reduced, further an effect on quality of a raw material in the cylinder mould is reduced, and meanwhile service life of the cylinder mould is prolonged.