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Soil remediation method based on pleurotus ostreatus and phanerochaete chrysosporium

A technology for Phanerochaete protothecoides and soil remediation, which is applied in the field of soil remediation based on oyster mushroom and Phanerochaete protothecoides, can solve the problems of poor induction effect, secondary pollution, insignificant increase in laccase production, etc. Reduce secondary pollution, reduce content, induce good effect

Inactive Publication Date: 2020-02-21
SOUTHWEST UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, exogenous compounds, such as aromatic substances, are commonly used to combine white rot fungi in pairs to induce an increase in laccase production. This method will cause secondary pollution, and the induction effect is not good, and the increase in laccase production is not obvious

Method used

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  • Soil remediation method based on pleurotus ostreatus and phanerochaete chrysosporium
  • Soil remediation method based on pleurotus ostreatus and phanerochaete chrysosporium
  • Soil remediation method based on pleurotus ostreatus and phanerochaete chrysosporium

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Experimental program
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Embodiment 1

[0040] Soil remediation method, the specific steps are:

[0041] (1) Culture medium preparation

[0042] PDA medium: Add 200g of potato starch, 10g of glucose and 20g of agar into 1L of sterile water, and stir evenly;

[0043] PDB medium: 200g of potato starch and 10g of glucose in 1L sterile water, stir well;

[0044] (2) Soil sample preparation

[0045] a. Clean soil samples: collected from the subsurface layer of the soil on the campus of Southwest University (depth 5cm-10cm), remove rhizomes, leaves, etc., air-dry indoors, and pass through a 5mm sieve; , volume ratio) was added to the sample mixed with pentachlorophenol soil, white rot fungi, and straw (wood) that had been cultivated for 5 days, shaken for 2 hours, and centrifuged (2500rpm / min, 10min) at 4°C for organic Separation from the solid phase; Ultrasonic treatment for 15 minutes to separate the organic solvent phase and the aqueous phase of the content; Rotate the ultrasonically treated sample until it is compl...

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Abstract

The invention belongs to the technical field of restoration of contaminated soil by a microbial method, and particularly relates to a soil remediation method based on pleurotus ostreatus and phanerochaete chrysosporium. In the soil remediation method, sterilized pine wood is used as a culture medium to cultivate pleurotus ostreatus and phanerochaete chrysosporium, and then a soil sample to be treated is added to continue the cultivation. The method cannot cause secondary pollution; the induction effect is good, and the laccase yield is significantly increased; and the content of pentachlorophenol in the soil can be significantly reduced.

Description

technical field [0001] The invention belongs to the technical field of recovering polluted soil by means of microorganisms, and in particular relates to a soil restoration method based on Pleurotus ostreatus and Phanerochaete chrysosporium. Background technique [0002] At present, my country's population accounts for 22% of the world's total, while forest reserves account for 3.2% of the world's total. With the acceleration of urban and rural construction and the continuous improvement of people's living standards, the consumption of wood has been increasing year by year. According to the current per capita wood consumption level of 1.16 cubic meters in developed countries, there is a huge gap in domestic wood resources. In recent years, my country's annual dependence on external timber has exceeded 40% ("Problems and countermeasures faced by the recycling of waste wood in my country", Tao Yiming, National Symposium on the Development of Wood-based Panel Industry Science a...

Claims

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Application Information

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IPC IPC(8): B09C1/10C12N9/02
CPCB09C1/105C12N9/0061C12Y110/03002
Inventor 何丙辉罗锋刘玉民钟紫旋
Owner SOUTHWEST UNIVERSITY
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