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Composite microbial agent used for degradation of garden waste, and preparation method and application thereof

A compound microbial agent and garden waste technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of pathogenic bacteria and heavy metal pollution, difficult to remove, etc.

Inactive Publication Date: 2017-09-15
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of the existing technologies are for the composting of agricultural waste, and it is often necessary to add manure, sludge, biogas residue, and kitchen waste to enhance the decomposing effect and adjust the nutrient balance.
However, the addition of these substances often brings about problems such as pathogenic bacteria and heavy metal pollution that are difficult to remove.
At present, there is a lack of microbial agents that can safely and efficiently degrade pure garden waste without additives and can be successfully applied in industry

Method used

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  • Composite microbial agent used for degradation of garden waste, and preparation method and application thereof
  • Composite microbial agent used for degradation of garden waste, and preparation method and application thereof
  • Composite microbial agent used for degradation of garden waste, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of composite microbial agent of the present invention

[0037] 1.1 Preparation of liquid medium

[0038] The composition and ratio of the liquid medium used in the present invention are as follows: potato 20%, sucrose 1%, glucose 1%, tryptone 0.3%, yeast extract 0.3% and the balance of water. After washing the potato, cut into pieces, press Proportionally add clean water (200g / 1L), boil, filter, add other components to the filtrate and make up the corresponding amount of water, adjust the pH to 6.8, and sterilize at 121°C for 20 minutes.

[0039] 1.2 Preparation of compound bacterial liquid

[0040] Bacillus subtilis CICC10088, Bacillus licheniformis CICC10095, Bacillus siamensis BNCC210681 and Bacillus tequilensis CICC10832 were selected and single colonies were inoculated into the liquid medium prepared in 1.1 at 37℃. Cultivate at 130r / min for 24 hours; inoculate Phanerochaete chrysosporium (fungi) CICC40719 in liquid medium, cultivate at 30℃, 130r / min ...

Embodiment 2

[0049] Example 2 Degradation laboratory experiment of the composite microbial agent of the present invention

[0050] 2.1 Cellulose Congo red medium degradation circle experiment

[0051] Four strains of Bacillus subtilis CICC10088, Bacillus licheniformis CICC10095, Bacillus siamese BNCC210681, and Bacillus tequila CICC10832 were respectively inoculated into the liquid medium prepared in 1.1 in Example 1, at 37°C and 130 r / min Cultivate for 24 hours; inoculate Phanerochaete chrysosporium BNCC210681 in another liquid medium prepared in 1.1 in Example 1, and cultivate for 48 hours at 30°C and 130 r / min.

[0052] After fermentation, each bacterial strain was inoculated into the same liquid medium for co-cultivation, the inoculum amount of each strain was 1% (v / v), and the bacterial liquid of Phanerochaete chrysosporium was separately inoculated into the liquid medium. It is 2% (v / v). After the inoculation, the two liquid media were fermented for another 24 hours at 45°C and 130r / min. ...

Embodiment 3

[0062] Example 3 Degradation experiment of composite microbial agent of the present invention

[0063] 3.1 Experimental materials and procedures

[0064] The composting material used in this embodiment is a composite of various plant dead leaves, which comes from Wanshou Park, Xicheng District, Beijing. The dead leaves are collected and crushed. The average size of the leaves is 0.5cm×0.5cm.

[0065] Set up the blank group CK and the experimental group X, and each group has three parallel experiments. The mass of the dead leaves of each group is uniformly 200kg, and the volume is about 0.8m 3 , The initial water content is 8.32%, and the initial pH is about 7.7. The experimental group inoculated the composite microbial inoculum prepared in Example 1 into the dead leaf pile of group X according to the inoculation amount of 1:12, and the blank control group was added with the same amount of sawdust, mixed and stirred evenly, and adjusted the final moisture content of the two groups o...

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Abstract

The invention relates to a composite microbial agent used for degradation of garden waste. The composite microbial agent is composed of composite bacterial liquid and a carrier, wherein the composite bacterial liquid comprises Bacillus subtilis, Bacillus licheniformis, Bacillus siamensis, Bacillus tequilensis and Phanerochaete chrysosporium. The bacterial strains of the composite microbial agent produce synergism, so the composite microbial agent can substantially degrade a variety of macro-molecular components in the garden waste, improve the decomposition degree of the waste, enhance the nutrient structure and microfloras of compost and reduce heavy metal pollution. The invention also relates to a preparation method for the composite microbial agent, a method for degrading and converting the garden waste by using the composite microbial agent and fertilizer prepared from products obtained after degradation and conversion of the garden waste with the composite microbial agent.

Description

Technical field [0001] The invention belongs to the technical field of environmental protection, and specifically relates to a composite microbial inoculum, and its preparation method and application. Background technique [0002] With the acceleration of urbanization and the continuous increase of urban gardens and green areas, a large amount of wastes such as litter and leaves are also generated while improving the environment, resulting in potential safety hazards and certain impacts on the ecological environment. Using microbial inoculants as decomposing starter to inoculate garden waste for composting treatment can achieve rapid decomposing of a large amount of garden waste, enhance fertilizer efficiency, improve soil microbial flora, reduce pathogenic bacteria and heavy metal pollution, etc. It is an effective waste treatment And resource utilization. [0003] However, most of the existing technologies are for the treatment of agricultural waste compost, and it is often nece...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C05G3/00A01G9/10C12R1/125C12R1/10C12R1/07C12R1/645
CPCC05F11/00C05F17/00C05G3/00C12N1/14C12N1/20C05F11/08Y02W30/40
Inventor 赵国柱赵恺凝国辉王晓旭
Owner BEIJING FORESTRY UNIVERSITY
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