Method used for accelerating lignin degradation in phanerochaete chrysosporium solid state fermentation

A technology of Phanerochaete chrysosporium and Phanerochaete, applied in the field of bioengineering

Inactive Publication Date: 2013-11-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these patented technologies all have a common problem, which is how to control the degradation of cellulose when the organism degrades lignin, because cellulose is the main raw material for bio-fermentation of ethanol, and only by controlling the degradation of cellulose in the biological pretreatment process can the biological efficiency be improved. Yield of ethanol, butanol and other fuels

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Production of bacteria on test tube slant

[0021] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Common Microbial Strain Preservation Management Center CCGMC5.776), 26°C, incubation time 50 hours; store at 4°C for later use.

[0022] 2. Production of primary strains

[0023] Mix bran and water at a ratio of 1:0.5 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 200g per bottle, and sterilize at 135°C for 30 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 21°C, and cultured statically for 115 hours.

[0024] 3. Preparation of secondary amplified strains

[0025] Mix corncob and water at a ratio of 1:0.5 (mass ratio), then add 5% soybean cake powder and 5% corn steep liquor (mass ratio) of the total weight of cornc...

Embodiment 2

[0031] 1. Production of bacteria on test tube slant

[0032] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Agricultural Microbiological Strain Preservation Management Center ACCC 30942), 26°C, incubation time 50 hours; store at 4°C for later use.

[0033] 2. Production of primary strains

[0034]Mix bran and water at a ratio of 1:1.8 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 132g per bottle, and sterilize at 124°C for 117 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 36°C, and cultured statically for 68 hours.

[0035] 3. Preparation of secondary amplified strains

[0036] Mix corncob and water at a ratio of 1:2.6 (mass ratio), then add 2.3% soybean cake powder and 2.2% corn steep liquor (mass ratio) of the total ...

Embodiment 3

[0042] 1. Production of bacteria on test tube slant

[0043] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Industrial Microbiology Strain Preservation Management Center CICC14067), 26°C, incubation time 50 hours; store at 4°C for later use.

[0044] 2. Production of primary strains

[0045] Mix bran and water at a ratio of 1:2.9 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 83g per bottle, and sterilize at 110°C for 170 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 48°C, and cultured statically for 25 hours.

[0046] 3. Preparation of secondary amplified strains

[0047] Mix corncob and water at a ratio of 1:3.9 (mass ratio), then add 0% soybean cake powder and 0% corn steep liquor (mass ratio) of the total weight of ...

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PUM

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Abstract

The invention discloses a method used for accelerating lignin degradation in phanerochaete chrysosporium solid state fermentation, and belongs to the field of bioengineering. The method comprises following steps: s traw is grinded, and is treated by a proper amount of hydrogen peroxide, and then the straw is inoculated with phanerochaete chrysosporium used for lignin degradation, so that lignin degradation in solid state fermentation is accelerated, and cellulose is seldom degraded in the processes. The method is simple, no pollution is caused to the environment, and resource waste is not caused. Straw processed by the method can be used as a raw material in production of bioethanol and biobutanol, or as a biological feed, so that utilization value of straw is increased.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the technology of using hydrogen peroxide to pretreat straw that has not been treated with high temperature and high pressure, to enhance the degradation of lignin in solid-state fermentation straw of Phanerochaete chrysosporium, and to inhibit the degradation of cellulose. Background technique [0002] Energy is an important material basis for economic and social development. Since the Industrial Revolution, the world's fossil energy resources have been consumed rapidly, and the ecological environment has continued to deteriorate. In particular, greenhouse gas emissions have led to increasingly severe global climate change, and the sustainable development of human society has been seriously threatened. Since the 1970s, the idea of ​​sustainable development has gradually become the consensus of the international community. The development and utilization of renewable energy has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08A01G1/04
Inventor 张志才
Owner JIANGSU UNIV
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