Method used for accelerating lignin degradation in phanerochaete chrysosporium solid state fermentation
A technology of Phanerochaete chrysosporium and Phanerochaete, applied in the field of bioengineering
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Embodiment 1
[0020] 1. Production of bacteria on test tube slant
[0021] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Common Microbial Strain Preservation Management Center CCGMC5.776), 26°C, incubation time 50 hours; store at 4°C for later use.
[0022] 2. Production of primary strains
[0023] Mix bran and water at a ratio of 1:0.5 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 200g per bottle, and sterilize at 135°C for 30 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 21°C, and cultured statically for 115 hours.
[0024] 3. Preparation of secondary amplified strains
[0025] Mix corncob and water at a ratio of 1:0.5 (mass ratio), then add 5% soybean cake powder and 5% corn steep liquor (mass ratio) of the total weight of cornc...
Embodiment 2
[0031] 1. Production of bacteria on test tube slant
[0032] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Agricultural Microbiological Strain Preservation Management Center ACCC 30942), 26°C, incubation time 50 hours; store at 4°C for later use.
[0033] 2. Production of primary strains
[0034]Mix bran and water at a ratio of 1:1.8 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 132g per bottle, and sterilize at 124°C for 117 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 36°C, and cultured statically for 68 hours.
[0035] 3. Preparation of secondary amplified strains
[0036] Mix corncob and water at a ratio of 1:2.6 (mass ratio), then add 2.3% soybean cake powder and 2.2% corn steep liquor (mass ratio) of the total ...
Embodiment 3
[0042] 1. Production of bacteria on test tube slant
[0043] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Technical Handbook of Industrial Microbiology Experiments" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Industrial Microbiology Strain Preservation Management Center CICC14067), 26°C, incubation time 50 hours; store at 4°C for later use.
[0044] 2. Production of primary strains
[0045] Mix bran and water at a ratio of 1:2.9 (mass ratio), keep the pH natural, pack into 500mL triangular flasks, 83g per bottle, and sterilize at 110°C for 170 minutes; after cooling, inoculate a ring of Phanerochaete chrysosporium test tube sclerotium spores , 48°C, and cultured statically for 25 hours.
[0046] 3. Preparation of secondary amplified strains
[0047] Mix corncob and water at a ratio of 1:3.9 (mass ratio), then add 0% soybean cake powder and 0% corn steep liquor (mass ratio) of the total weight of ...
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