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Method for pre-processing corn stalks through physical-chemical-biological method

A biological pretreatment and corn stalk technology, applied in the field of biochemistry, can solve the problems of long biological treatment cycle, environmental pollution, high energy consumption, etc., and achieve the effect of improving the utilization efficiency, simple process and simple equipment

Inactive Publication Date: 2016-01-20
刘云平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because each treatment method has certain defects, such as chemical and physical treatment have problems of environmental pollution and high energy consumption, while biological treatment has a long cycle and low efficiency

Method used

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  • Method for pre-processing corn stalks through physical-chemical-biological method
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  • Method for pre-processing corn stalks through physical-chemical-biological method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Pretreat corn stalks according to the following steps

[0023] (1) Corn stalks are crushed through a pulverizer first, and then passed through a 40-mesh sieve;

[0024] (2) Treat the crushed corn stalks with a NaOH solution with a mass concentration of 1.0%, with a solid-to-liquid ratio of 1:10, filter at 100°C for 1 hour, wash the filter residue with water to neutrality, and dry it to a constant weight as a fermentation substrate;

[0025] (3) First prepare every 10mL containing FeSO 4 ·7H 2 O0.0005g, MnSO 4 ·7H 2 O0.0016g, ZnSO 4 ·7H 2 O0.0014g, CoCl 2 .0.002g of trace element mixed solution; secondly prepare nutrient solution, the mass percent concentration of each component in the nutrient solution is: (NH 4 ) 2 SO 4 1%, KH 2 PO 4 0.3%, MgSO 4 0.05%, CaCl 2 0.05%, the rest is water; then prepare the mixed solution, which is prepared by adding 1mL of trace element mixed solution to every 100mL of nutrient solution; add the mixed solution to...

Embodiment 2

[0027] Example 2: Steps (1), (2) are the same as in Example 1; the preparation of the mixed solution in step (3) is the same as in Example 1; add and mix to the fermentation substrate in step (2) according to the material-to-liquid ratio of 1:2.25 liquid, adjust the pH to 5.5, and sterilize at 121°C for 20 minutes to obtain a solid-state fermentation medium; 6The Phanerochaete chrysosporium spore suspension of each / mL was inserted into the solid-state fermentation medium with an inoculum size of 10% by mass, and fermented for 10 days; 6 Trichoderma viride spore suspension per mL was cultured at 28°C for 4 days to obtain the pretreated product.

[0028] Such as figure 2 As shown in group 1, the degradation rates of lignin, hemicellulose and cellulose in the straw were 98.1%, 77.1% and 41.6%, respectively.

Embodiment 3

[0029] Example 3: Steps (1), (2) are the same as in Example 1; the preparation of the mixed solution in step (3) is the same as in Example 1; add and mix to the fermentation substrate in step (2) according to the material-to-liquid ratio of 1:2.25 liquid, adjust the pH to 5.5, and sterilize at 121°C for 20 minutes to obtain a solid-state fermentation medium; 6 The Phanerochaete chrysosporium spore suspension of each / mL was inserted into the solid-state fermentation medium with an inoculum size of 10% by mass, and fermented for 10 days; 6 pcs / mL Trichoderma asperelloides The spore suspension was cultured at 28°C for 4 days to obtain the pretreated product.

[0030] Such as figure 2 As shown in group 2, the degradation rates of lignin, hemicellulose and cellulose in the straw were 98.8%, 81.7% and 44.2%, respectively.

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Abstract

The invention relates to a method for pre-processing corn stalks through a physical-chemical-biological method. The corn stalks are firstly smashed and physically processed, then the corn stalks are chemically processed through dilute alkali, and finally, the corn stalks are biologically processed through phanerochaete chrysosporium and cellulose degradation bacteria. Lignin removal is facilitated due to dilute alkali processing, the corn stalks can be fully degraded easily because of mixed fermentation, the utilizing rate is increased, and microorganisms producing ethyl alcohol are inoculated into the pre-processed stalks to produce ethyl alcohol, or feeding yeast is inoculated to produce protein feed. The method is simple in technology and convenient to implement.

Description

technical field [0001] The invention relates to a method for pretreatment of corn stalks, in particular to a method for physical-chemical-biological pretreatment of corn stalks, belonging to the technical field of biochemistry. Background technique [0002] Straw is a very abundant renewable energy source, mainly composed of cellulose, hemicellulose and lignin. Among them, lignin is a high-molecular aromatic compound that cannot be hydrolyzed into sugar, while cellulose and hemicellulose are potential sources of fermentable sugar. Saccharification (enzymatic hydrolysis) of plant straws into fermentable sugars and production of fuel alcohol, single-cell proteins and organic acids through microbial fermentation is considered to be one of the effective ways to utilize plant straws, but cellulose, hemicellulose and Lignin is intertwined with each other to form a cell wall structure and bind the cells together. This intertwined structure determines that the degradation of any ty...

Claims

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Application Information

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IPC IPC(8): C12P7/10A23K10/30
CPCY02E50/10Y02P60/87
Inventor 方芳赵玉萍
Owner 刘云平
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