Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof
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A technology for furfural residue and xylose residue is applied in the field of xylose residue or furfural residue pretreatment bacterial agent and preparation thereof, and can solve the problems of difficulty in degradation, influence on gas production efficiency, and high content of cellulose hemicellulose and lignin
Inactive Publication Date: 2011-08-17
ENERGY RES INST OF SHANDONG ACAD OF SCI
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Problems solved by technology
Due to the high content of cellulose, hemicellulose and lignin in xylose residue or furfural r...
Method used
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Embodiment 1
[0027] Trichoderma viride ( Trichoderma viride ), Phanerochaete chrysosporium ( Phanerochaete chrysosporium ), Aspergillus niger ( Aspergillus niger ), Aspergillus oryzae ( Aspergillus oryzae ), Trametes versicolor ( Trametes versicolor ) can be obtained from the China General Microorganism Culture Collection Center (CGMCC). 5.776, Trametes versicolor CGMCC number is 5.48 or 5.161, Aspergillus niger CGMCC number is one of 3.6479, 3.6478, 3.6477, 3.6476, 3.6475, 3.6329; Aspergillus oryzae is one of 3.5232, 3.4437, 3.4383, 3.4382, 3.4259, 3.2140 . Five kinds of bacterial liquids were obtained after conventional liquid-state pure-bred fermentation. The dry weight of the bacteria reached 20 grams per liter. The specific implementation method is as follows:
[0028] 1) The above five microorganisms were activated with their respective activation slant medium, and cultured at 30°C for 8 days.
[0029] 2) Inoculate the spore suspension of the above-mentioned activated str...
Embodiment 2
[0038] The preparation method of the bacterial liquid of five kinds of bacteria is as described in Example 1.
[0039] 3 parts xylose residue, 1 part diatomaceous earth, 1 part peat. After sterilizing the xylose residue, diatomaceous earth and peat, mix them evenly, and then add microbial agents (Trichoderma viride 0.30, Phanerochaete chrysosporium 0.10, Aspergillus niger 0.06, Aspergillus oryzae 0.16, discoloration Trametes bacteria solution 0.05.), after mixing evenly, the composite solid bacteria agent is obtained.
Embodiment 3
[0041] The bacterial liquid preparation of five kinds of bacteria is as described in Example 1.
[0042] 1 part of furfural residue, 2 parts of peat, 2 parts of diatomaceous earth, after sterilization, add to the mixer and add microbial agents (0.20 of Trichoderma viride, 0.15 of Phanerochaete chrysosporium, 0.05 of Aspergillus niger, rice koji Mold liquid 0.10, Trametes versicolor bacterial liquid 0.10.), after mixing evenly, the composite solid bacterial agent is obtained.
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Abstract
The invention relates to a pretreatment fungicide for xylose residue or furfural residue, a preparation method and an application thereof, belonging to the technical field of agricultural microbiology. The invention relates to a microbial composite fungicide specially used for promoting the xylose residue or the furfural residue to degrade and improving the production efficiency of methane. The invention firstly adopts liquid fermentation technology to purely breed and ferment all components of the microbial fungicide, and then the components are compounded. The preparation method of the pretreatment fungicide comprises: 0.20-0.40 parts by weight of trichoderma viride liquid, 0.10-0.15 parts by weight of phanerochaete chrysosporium liquid, 0.05-0.10 parts by weight of aspergillus niger liquid, 0.08-0.16 parts by weight of aspergillus oryzae liquid and 0.05-0.10 parts by weight of trametes versicolor liquid, and the dry weight of fermentation hyphae is more than 20g/L. The composite fungicide can be used for improving the composite fungicide speed of the xylose residue or the furfural residue as well as the methane production efficiency; the methane production efficiency is improved by 30-60%; the fermentation time is shortened; the start period is shortened by 3-10 days; and the invention is also good in the effects on the aspects of pretreatment of smashed straws, and methane production.
Description
technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a pretreatment bacterial agent for xylose residue or furfural residue, a preparation method and application thereof. Background technique [0002] With the rapid development of my country's economy, the demand for energy has increased year by year, and the problem of energy shortage has become increasingly prominent. At present, my country is vigorously developing renewable energy including biogas to cope with the shortage of fossil energy and realize the sustainable development of my country's economy. In terms of biogas raw materials, in addition to livestock and poultry manure, the most eye-catching is the use of crop straw to produce biogas. However, straw itself is difficult to fully degrade, which seriously affects its gas production efficiency and application range, and it is still in the research and development stage. Xylose residue is a...
Claims
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