Pretreatment fungicide for xylose residue or furfural residue, preparation method and application thereof
A technology for furfural residue and xylose residue is applied in the field of xylose residue or furfural residue pretreatment bacterial agent and preparation thereof, and can solve the problems of difficulty in degradation, influence on gas production efficiency, and high content of cellulose hemicellulose and lignin
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Embodiment 1
[0027] Trichoderma viride ( Trichoderma viride ), Phanerochaete chrysosporium ( Phanerochaete chrysosporium ), Aspergillus niger ( Aspergillus niger ), Aspergillus oryzae ( Aspergillus oryzae ), Trametes versicolor ( Trametes versicolor ) can be obtained from the China General Microorganism Culture Collection Center (CGMCC). 5.776, Trametes versicolor CGMCC number is 5.48 or 5.161, Aspergillus niger CGMCC number is one of 3.6479, 3.6478, 3.6477, 3.6476, 3.6475, 3.6329; Aspergillus oryzae is one of 3.5232, 3.4437, 3.4383, 3.4382, 3.4259, 3.2140 . Five kinds of bacterial liquids were obtained after conventional liquid-state pure-bred fermentation. The dry weight of the bacteria reached 20 grams per liter. The specific implementation method is as follows:
[0028] 1) The above five microorganisms were activated with their respective activation slant medium, and cultured at 30°C for 8 days.
[0029] 2) Inoculate the spore suspension of the above-mentioned activated str...
Embodiment 2
[0038] The preparation method of the bacterial liquid of five kinds of bacteria is as described in Example 1.
[0039] 3 parts xylose residue, 1 part diatomaceous earth, 1 part peat. After sterilizing the xylose residue, diatomaceous earth and peat, mix them evenly, and then add microbial agents (Trichoderma viride 0.30, Phanerochaete chrysosporium 0.10, Aspergillus niger 0.06, Aspergillus oryzae 0.16, discoloration Trametes bacteria solution 0.05.), after mixing evenly, the composite solid bacteria agent is obtained.
Embodiment 3
[0041] The bacterial liquid preparation of five kinds of bacteria is as described in Example 1.
[0042] 1 part of furfural residue, 2 parts of peat, 2 parts of diatomaceous earth, after sterilization, add to the mixer and add microbial agents (0.20 of Trichoderma viride, 0.15 of Phanerochaete chrysosporium, 0.05 of Aspergillus niger, rice koji Mold liquid 0.10, Trametes versicolor bacterial liquid 0.10.), after mixing evenly, the composite solid bacterial agent is obtained.
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