Microbial agent for pretreatment of straws and manure biogas

A technology of microbial inoculum and biogas fermentation, applied in microorganisms, microorganism-based methods, fermentation, etc., can solve the problems of incomplete research on bacterial antagonism, difficult to achieve ideal effects, and inability to play a role, and promote the mixing of straw and manure. Anaerobic fermentation process, shortened fermentation cycle, easy to implement effect

Inactive Publication Date: 2014-10-01
NANJING YUANYI ENVIRONMENT BIOLOGICAL ENGCO
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

There are big problems in this method: First, these single bacteria do have certain functions when cultured in the laboratory, but it is difficult to achieve the desired effect under the complex conditions of fermentation
This is because many single strains cannot grow and reproduce in the actual environment or are susceptible to the growth of other microorganisms; secondly, because there are many interactions between different microbial groups, the strains screened in the laboratory have certain functions. A single bacterium does not fully prove that it has the same effect in a complex group; third, some microbial biogas fermentation agents simply mix the fermentation strains together, and the antagonism between the strains is not thoroughly studied , which leads to the microbial strains in the fermentation process due to the antagonism between the strains and can not achieve the desired effect; the fourth, can not effectively use the role of various enzymes produced by microorganisms
In the degradation process of organic matter, the degradation of enzymes is very critical, therefore, microbial agents that only contain microorganisms but do not contain enzymes cannot play a greater role

Method used

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  • Microbial agent for pretreatment of straws and manure biogas
  • Microbial agent for pretreatment of straws and manure biogas

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Straw and manure biogas fermentation pretreatment The microorganisms in the microbial agent are mainly screened from various cellulose-degrading bacteria and lignin-degrading bacteria in the fermentation biogas slurry, various bacteria, actinomycetes, and fungi in mature compost and tree soil And get.

[0023] (1) Separation and screening of microorganisms

[0024] For the separation and screening of cellulose-degrading bacteria and lignin-degrading bacteria, the existing biogas fermentation tank in the laboratory was used as the source of microorganisms, and straw powder was used as the only carbon source to prepare inorganic salt medium, and the dilution plate method was used to separate different microorganisms. After culturing at 32°C, 12 strains of aerobic and facultative anaerobic bacteria were isolated. Then, use each microorganism to pretreat the straw, and measure the overall degradation rate of cellulose, hemicellulose, and lignin to screen out six types with...

Embodiment 3

[0050] The dominant bacterial agent (microorganism No. 6 in Example 2) screened out according to the amount of gas produced was mixed with straw in different proportions for pretreatment, and placed in a constant temperature incubator at 37°C for anaerobic fermentation. According to the amount of biogas, the optimal feed ratio (mass ratio) of inoculant to straw is finally determined.

[0051] The mass ratio of dry powder inoculum to straw was 1:1000 for No. 1, 1:500 for No. 2, 1:100 for No. 3, 1:50 for No. 4 and 1:20 for No. 5, and 15 g of straw was uniformly added.

[0052] Experiments show that the gas production is the highest when the mass ratio of dry powder bacterial agent to straw is 1:50. Therefore, choose 1:50 as the best feed ratio.

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Abstract

The invention discloses a microbial agent for pretreatment of straws and manure biogas. The microbial agent jointly comprises the following strains: 2-8*10<10>cfu / g of Penicillium glaucum, 2-8*10<10>cfu / g of Rhizopus oryzae, 2-8*10<10>cfu / g of Phanerochaete chrysosporium, 2-8*10<10>cfu / g of Aspergillus flavus and 2-8*10<10>cfu / g of Spirillospora. By virtue of the microbial agent, the mixed anaerobic fermentation process of straws and manure can be promoted, the fermentation period is shortened, the biodegradation of organic materials can be accelerated, and a preparation method of the microbial agent is simple and easily implemented, the content of flora in feedstock per unit is large and the activity is high. The microbial agent can be used for the fermentation of different manure and straw raw materials in biogas fermentation enterprises and thus the harmless, reduction and resource utilization of manure and straws are achieved.

Description

technical field [0001] The invention belongs to the technical field of fermentation pretreatment bacterial agents for agricultural waste, and in particular relates to a microbial bacterial agent for fermenting pretreatment of straw and feces biogas. Background technique [0002] At present, my country's annual output of straw is about 700 million tons, and the excrement discharge of livestock and poultry farming is about 1.73 billion tons. A large number of agricultural wastes are not used rationally, and most of them are directly burned or discarded in fields and rivers, which not only causes waste of resources but also seriously damages the ecological environment. Studies have shown that the potential of fermentation gas production using straw as raw material is about 0.45m 3 / (Kg·Ts), which is equivalent to the fermentation rate of feces, and its gas production potential is very huge. Using straw as raw material to ferment biogas not only solves the problem of energy sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C12P5/02C05F17/00C02F11/04C12R1/80C12R1/845C12R1/645C12R1/67C12R1/01
CPCY02E50/30Y02W10/20Y02W30/40
Inventor 乔维川张瑞张晓玉
Owner NANJING YUANYI ENVIRONMENT BIOLOGICAL ENGCO
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