A microbial agent for pretreatment of straw and manure biogas fermentation
A microbial agent, biogas fermentation technology, applied in the direction of microorganisms, microorganism-based methods, fermentation, etc., can solve the problems of incomplete research on the antagonism of strains, difficulty in achieving the desired effect, and inability to play a role, so as to promote the mixing of straw and feces Anaerobic fermentation process, shortened fermentation cycle, and easy implementation
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Embodiment 1
[0022] Straw and manure biogas fermentation pretreatment The microorganisms in the microbial agent are mainly screened from various cellulose-degrading bacteria and lignin-degrading bacteria in the fermentation biogas slurry, various bacteria, actinomycetes, and fungi in mature compost and tree soil And get.
[0023] (1) Separation and screening of microorganisms
[0024] For the separation and screening of cellulose-degrading bacteria and lignin-degrading bacteria, the existing biogas fermentation tank in the laboratory was used as the source of microorganisms, and straw powder was used as the only carbon source to prepare inorganic salt medium, and the dilution plate method was used to separate different microorganisms. After culturing at 32°C, 12 strains of aerobic and facultative anaerobic bacteria were isolated. Then, use each microorganism to pretreat the straw, and measure the overall degradation rate of cellulose, hemicellulose, and lignin to screen out six types with...
Embodiment 3
[0050] The dominant bacterial agent (microorganism No. 6 in Example 2) screened out according to the amount of gas produced was mixed with straw in different proportions for pretreatment, and placed in a constant temperature incubator at 37°C for anaerobic fermentation. According to the amount of biogas, the optimal feed ratio (mass ratio) of inoculant to straw is finally determined.
[0051] The mass ratio of dry powder inoculum to straw was 1:1000 for No. 1, 1:500 for No. 2, 1:100 for No. 3, 1:50 for No. 4 and 1:20 for No. 5, and 15 g of straw was uniformly added.
[0052] Experiments show that the gas production is the highest when the mass ratio of dry powder bacterial agent to straw is 1:50. Therefore, choose 1:50 as the best feed ratio.
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