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Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium

A technology of Phanerochaete and Phanerochaete spheroids, which is applied in the application field of Phanerochaete chrysosporium in the treatment of heavy metal and organic pollutant wastewater, can solve the problems of toxicity response mechanism and removal characteristics, and achieve treatment Low process cost, strong practicability, easy to expand the effect of cultivation

Inactive Publication Date: 2011-04-20
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the toxicity response mechanism and removal characteristics of microorganisms to the combined pollution of heavy metals and organic pollutants are still unclear, there is no precedent for the simultaneous application of microorganisms to wastewater contaminated by heavy metals and organic pollutants.

Method used

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  • Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium
  • Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium
  • Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium

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Effect test

Embodiment 1

[0018] A method of utilizing Phanerochaete chrysosporium to simultaneously remove cadmium and 2,4-dichlorophenol in waste water of the present invention comprises the following steps:

[0019] 1. Preparation of Phanerochaete chrysosporium bacterial balls

[0020] The spore powder of Phanerochaete chrysosporium BKM-F1767 (the preservation number in the American Type Culture Collection is ATCC 24725, preferably adopting this strain, but not limited to this) is scraped from the slant medium, and prepared in sterile water. Spore suspension. The spore suspension was inoculated into the liquid culture medium (Kirk medium) of the Erlenmeyer flask, and 200 mL of the liquid culture medium was housed in the 500 mL Erlenmeyer flask, and cultivated in a constant temperature shaking box at a culture temperature of 37 ° C and a shaking table speed of 150 rpm. Phanerochaete chrysosporium spheroids were obtained after culturing for 72 hours.

[0021] 2. Treat wastewater containing cadmium a...

Embodiment 2

[0029] A method of utilizing Phanerochaete chrysosporium to simultaneously remove cadmium and 2,4-dichlorophenol in waste water of the present invention comprises the following steps:

[0030] 1. Preparation of Phanerochaete chrysosporium bacterial balls

[0031] This step is the same as Step 1 of Example 1.

[0032] 2. Treat wastewater containing cadmium and 2,4-dichlorophenol

[0033] The initial concentration of 2,4-dichlorophenol is 20mg / L, the initial concentration of cadmium is respectively 2mg / L, 5mg / L, 10mg / L, 15mg / L, 20mg / L and 30mg / L six treat Add the above-mentioned Phanerochaete chrysosporium bacterial balls prepared above in the treatment wastewater sample, the amount added in every liter of waste water is 0.4g based on the dry weight of Phanerochaete chrysosporium bacterial balls, and the pH value is adjusted to 6.5, at 37 Under the temperature condition of ℃, shake the shaker with a rotating speed of 150rpm for 96 hours, filter out the bacterial balls in the w...

Embodiment 3

[0038] A method of utilizing Phanerochaete chrysosporium to simultaneously remove cadmium and 2,4-dichlorophenol in waste water of the present invention comprises the following steps:

[0039] 1. Preparation of Phanerochaete chrysosporium bacterial balls

[0040] This step is the same as Step 1 of Example 1.

[0041] 2. Treat wastewater containing cadmium and 2,4-dichlorophenol

[0042]The initial concentration of cadmium is 2mg / L respectively, and the initial concentration of 2,4-dichlorophenol is respectively 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L and 100mg / L six treat Add the above-mentioned Phanerochaete chrysosporium bacterial balls prepared above in the treatment wastewater sample, the amount added in every liter of waste water is 0.4g based on the dry weight of Phanerochaete chrysosporium bacterial balls, and the pH value is adjusted to 6.5, at 37 Under the temperature condition of ℃, shake the shaker with a rotating speed of 150rpm for 96 hours, filter out the bacteri...

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Abstract

The invention discloses a method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium, comprising the following steps: adding P.chrysosporium pellets into the wastewater containing cadmium and dichlorophenol, wherein the addition amount in each liter of wastewater is 0.3-0.5g in terms of the dry weight of the P.chrysosporium pellets; then, adjusting the pH value of the wastewater to 3.5-8.5, and performing an absorption degradation reaction at the constant temperature of 35-39 DEG C, wherein the revolving speed is controlled at 140-160rpm and the reaction time is not less than 36h during the reaction; and filtering the P.chrysosporium pellets after the reaction, thereby removing the cadmium and dichlorophenol in the wastewater simultaneously. The invention has the advantages of simple operation conditions, wide application range, no need of reformation and pretreatment for the adopted P.chrysosporium pellets, low cost, high microorganism utilization factor and the like, and is easy to implement.

Description

technical field [0001] The invention relates to the field of microorganism application and waste water treatment, in particular to the application of Phanerochaete chrysosporium in treating heavy metal and organic pollutant waste water. Background technique [0002] At present, the harm of combined pollution of heavy metals and organic pollutants in the environment has become a global problem. Among them, cadmium in heavy metals is highly toxic and has serious harm to the growth and development of humans, animals and plants. Therefore, how to remove cadmium pollution has been highly concerned by environmental protection researchers. Cadmium-containing wastewater mainly comes from electroplating factories, dye industries, mining and metallurgy processes, battery production, tanning, film manufacturing, and automobile manufacturing. In fact, there are usually a series of organic pollutants in the cadmium-containing wastewater discharged from the above-mentioned sectors, such...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 曾光明陈安伟陈桂秋范佳琦邹正军张文娟王亮
Owner HUNAN UNIV
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