Complex microbial agent for composting fermentation and application thereof

A compound bacterial agent and composting technology, which is applied in the field of biological fermentation and microorganisms, can solve the problems of not reaching the compound bacterial agent, the total number of live bacteria in the product is not high, and the strain is not functional, so as to reduce unpleasant odor and improve the quality of compost products , The effect of reducing the cost of agricultural production

Inactive Publication Date: 2014-01-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common problem at present is that in the process of compound microbial screening and cultivation, there is a lack of basic theoretical guidance, resulting in weak functionalities of the screened strains; T...

Method used

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  • Complex microbial agent for composting fermentation and application thereof
  • Complex microbial agent for composting fermentation and application thereof
  • Complex microbial agent for composting fermentation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1.1 Microbial agent preparation

[0024] 1.1.1 Incline culture: the original strains of Phanerochaete chrysosporium, Streptomyces grisea, Bacillus subtilis, methylotrophic Bacillus and Bacillus amyloliquefaciens (the above-mentioned strains are common strains in the art, and can be obtained commercially) were respectively inoculated on corresponding solid medium, Phanerochaete chrysosporium was cultivated at 28-30° C. for 3-5 days, Streptomyces grisea was cultivated at 28-30° C. for 5-7 days, and Bacillus subtilis, methylotrophic Bacillus and Bacillus amyloliquefaciens are cultured at 30-37°C for 2-3 days to activate the strains.

[0025] 1.1.2 Primary seed culture: Wash the activated slant with sterile physiological saline and transfer it to a sterile Erlenmeyer flask for shaking culture. Bacillus subtilis, methylotrophic Bacillus and Bacillus amyloliquefaciens were cultured at 30°C and 180r / mmin for 48 hours to obtain a first-grade seed solution;

[0026] Phanerocha...

Embodiment 2

[0045] The difference from Example 1 is that the mixing ratio during the preparation of the composite bacterial agent is: solid bacterial agents of Phanerochaete chrysosporium, Streptomyces grisea, Bacillus subtilis, methylotrophic Bacillus and Bacillus amyloliquefaciens according to 2:2:1:1:1 ratio mix well.

[0046] Composting raw materials and methods, sampling and assay methods are all the same as in Example 1.

[0047] 2.1 Experimental results

[0048] 2.1.1 Changes in temperature during composting

[0049] The compost inoculated with bacteria also enters the pyrolysis stage (>45°C) on the second day after composting (see figure 2 ), the high temperature period lasted 18 days, 2 days longer than Example 1.

[0050] 2.1.2 Changes in lignocellulose content during composting

[0051] It can be seen from Table 2 that at the end of composting, the degradation rates of cellulose, hemicellulose and lignin in the compost added with bacterial agents increased by 32.5%, 22.48%...

Embodiment 3

[0055] The difference from Example 1 is that the mixing ratio during the preparation of the composite bacterial agent is: solid bacterial agents of Phanerochaete chrysosporium, Streptomyces grisea, Bacillus subtilis, methylotrophic Bacillus and Bacillus amyloliquefaciens according to 2:1:2:1:2 ratio mix well.

[0056] Composting raw materials and methods, sampling and assay methods are all the same as in Example 1.

[0057] 2.1 Experimental results

[0058] 2.1.1 Changes in temperature during composting

[0059] The compost inoculated with bacteria also enters the pyrolysis stage (>45°C) on the second day after composting (see image 3 ), the high temperature period lasted 17 days, which was extended by 1 day than in Example 1.

[0060] 2.1.2 Changes in lignocellulose content during composting

[0061] It can be seen from Table 3 that at the end of composting, the degradation rates of cellulose, hemicellulose and lignin in the compost added with bacterial agents increased ...

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Abstract

The invention discloses a complex microbial agent for composting fermentation and application thereof. The complex microbial agent is a solid inoculant which comprises phanerochaete chrysosporium, streptomyces griseorubens, bacillus subtilis, bacillus methylotrophicus and bacillus amyloliquefaciens. The complex microbial agent is a complex microbial system comprising various microorganisms which have complementary and synergistic functions; the microbial system integrates quick fermentation heat production, biological deodorization and compost material decomposition and degradation and has the effects of a biological fertilizer; the livestock manure compost can be quickly heated to kill off harmful organisms at high temperature; the unpleasant smell is obviously reduced to solve the problem of environment pollution caused by poultry excrement; the microorganisms can be quickly propagated in the poultry excrement fermentation process; the organic matters such as lignocellulose in the excrement can be quickly and effectively decomposed so as to increase the decomposition speed of the compost material and improve the quality of the compost product; the complex microbial agent can be used for producing the biological organic fertilizer so as to reduce the agricultural production cost.

Description

technical field [0001] The invention belongs to the field of biological fermentation and microorganisms, and in particular relates to a composite microbial agent for composting fermentation and its application. Background technique [0002] With the rapid and intensified development of the dairy farming industry, the environmental pollution caused by improper handling of cow manure is becoming more and more serious. At present, the vast majority of farmers directly apply cow dung to farmland as fertilizer, pile it up in the open air or flush it into ponds, ditches, etc., resulting in the increasingly serious problem of manure pollution. From an environmental point of view, it pollutes the atmosphere, soil, and waters, deteriorates the ecological environment and the living environment of residents, endangers human health, and is not conducive to the healthy growth of dairy cows. But from the perspective of resources, it is a good renewable resource. If it can be used scienti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F3/00C12R1/645C12R1/465C12R1/125C12R1/07
CPCY02A40/20Y02W30/40
Inventor 许修宏徐杰李洪涛刘华晶金羽
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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