Maize stalk degradation and acidification species inoculant and manufacture method thereof

The technology of corn stalk and compound bacterial agent is applied in the biological field, which can solve the problems of loss of dissolved organic matter, environmental pollution, influence on subsequent utilization of biogas residue, etc., and achieves the effect of high enzyme activity and broad application prospect.

Inactive Publication Date: 2018-04-20
ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used acid-base pretreatment methods are mainly strong acid or strong alkali pretreatment, such as hydrochloric acid, sulfuric acid, sodium hydroxide, potassium hydroxide, this pretreatment method generally has the problem of equipment corrosion, and requires a large amount of water for the pretreatment. The processed raw materials need to be cleaned or need a large amount of acid and alkali to neutralize
When washing with water, soluble organic matter will be lost, and when n...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Activation of bacteria

[0031] Aspergillus niger (Aspergillus) ACCC No. 30171 was inoculated in Sabouraud Dextrose Broth (SDB), cultured at 26-28° C. and 150-220 rpm in a shaking environment for 24-60 hours, and all the fermentation broth was collected.

[0032] Trichoderma ACCC No. 31490 was inoculated in Sabouraud Dextrose Broth (SDB), cultured at 26-28° C. and 150-220 rpm in a shaking environment for 24-60 hours, and all the fermentation broth was collected.

[0033] Penicillium oxalicum (Penicllium) ACCC No. 32576 was inoculated in Sabouraud's Dextrose Broth (SDB), cultured at 26-28° C. and shaken at 150-220 rpm for 24-60 hours, and all the fermentation broth was collected.

[0034] Inoculate Phanerochaete chrysosporium (Phanerochaete chrysosporium) CICC No.40299 in Sabouraud Dextrose Broth (SDB), culture at 26-28°C, 150-220rpm shaking environment for 24-60h, and collect all fermented liquid.

[0035] (2) Expanded culture of bacteria

[0036] Inoculate the f...

Embodiment 2

[0040] Example 2, Straw Degradation Acidification Bacteria Applied to Acidification Pretreatment of Corn Stalk Anaerobic Fermentation

[0041] Experimental materials: corn stalks, the straw-degrading acidifying bacterial agent prepared in Example 1;

[0042] Treatment 1: without adding the straw-degrading acidifying bacterial agent prepared in Example 1.

[0043] Treatment 2: use the straw-degrading acidifying bacterial agent prepared in Example 1.

Embodiment approach

[0044] Implementation method: Two groups of experiments were designed. One group of experiments used 660ml of bacterial agent, 20g of yellow storage corn stalks crushed to 1-2cm, and 180rpm shaking culture; the other group did not add bacterial agent, and ensured that other experimental conditions were the same.

[0045] The content of cellulose, hemicellulose and volatile organic acid was detected every day, and the degradation rate was calculated. The detection methods of cellulose, hemicellulose and volatile organic acids are based on literature: "Determination of cellulose_hemicellulose and lignin in corn straw", Wang Jinzhu, Wang Yuanxiu, Li Feng, Gao Yanhua, Xu Junqing, Yuan Jianguo, Shandong Food Fermentation , 2010.3 (total issue 158), cellulose degradation rate = decomposed cellulose amount / total cellulose amount, hemicellulose degradation rate = decomposed hemicellulose amount / total hemicellulose amount, lignin degradation rate = decomposed Decomposed lignin amount / t...

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PUM

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Abstract

The invention discloses maize stalk degradation and acidification species inoculant and a manufacture method thereof. Multiple species inoculant for degrading maize stalks comprises the following active components: aspergillus, trichoderma, penicillium and phanerochaete chrysosporium. The species inoculant breaks through the limitation that single fungus and clastic enzyme thereof cannot rapidly and efficiently decompose natural cellulose, and provides a key pretreatment technology for natural cellulose methane fermentation. By treating maize stalks through the method for 15 days continuously,the degradation rate of cellulose of the maize stalks reaches 42.1 percent, the degradation rate of hemicelluloses reaches 46.7 percent, the degradation rate of lignin reaches 28.5 percent, and the method has a wide application prospect when being applied to the field of methane preparation by taking cellulose and lignin, such as maize stalks, as raw materials.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a corn stalk-degrading acidifying bacterial agent and a preparation method thereof. Background technique [0002] Crop straw is the fourth largest energy source in the world after coal, oil, and natural gas, and is a very important renewable biomass resource. my country is a large agricultural country with extremely rich crop straw resources. The annual output of straw in the world is as much as 3 billion tons, while the annual output of crop straw in my country has exceeded 800 million tons. Most of the straw produced in our country is burned on the spot or used as living fuel, which not only pollutes the environment, but also wastes resources, and the straw has not been effectively utilized. In addition, the high temperature generated by incineration will also kill microorganisms in the soil, which is not conducive to the survival of soil microorganisms, and will also cause soil c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/10C12R1/685C12R1/885C12R1/80C12R1/645
CPCC12N1/14C12P7/10C12P2201/00Y02E50/10
Inventor 赵立欣黄开明姚宗路冯晶于佳动罗娟
Owner ACADEMY OF PLANNING & DESIGNING OF THE MINIST OF AGRI
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