Maize stalk degradation and acidification species inoculant and manufacture method thereof
The technology of corn stalk and compound bacterial agent is applied in the biological field, which can solve the problems of loss of dissolved organic matter, environmental pollution, influence on subsequent utilization of biogas residue, etc., and achieves the effect of high enzyme activity and broad application prospect.
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Embodiment 1
[0030] (1) Activation of bacteria
[0031] Aspergillus niger (Aspergillus) ACCC No. 30171 was inoculated in Sabouraud Dextrose Broth (SDB), cultured at 26-28° C. and 150-220 rpm in a shaking environment for 24-60 hours, and all the fermentation broth was collected.
[0032] Trichoderma ACCC No. 31490 was inoculated in Sabouraud Dextrose Broth (SDB), cultured at 26-28° C. and 150-220 rpm in a shaking environment for 24-60 hours, and all the fermentation broth was collected.
[0033] Penicillium oxalicum (Penicllium) ACCC No. 32576 was inoculated in Sabouraud's Dextrose Broth (SDB), cultured at 26-28° C. and shaken at 150-220 rpm for 24-60 hours, and all the fermentation broth was collected.
[0034] Inoculate Phanerochaete chrysosporium (Phanerochaete chrysosporium) CICC No.40299 in Sabouraud Dextrose Broth (SDB), culture at 26-28°C, 150-220rpm shaking environment for 24-60h, and collect all fermented liquid.
[0035] (2) Expanded culture of bacteria
[0036] Inoculate the f...
Embodiment 2
[0040] Example 2, Straw Degradation Acidification Bacteria Applied to Acidification Pretreatment of Corn Stalk Anaerobic Fermentation
[0041] Experimental materials: corn stalks, the straw-degrading acidifying bacterial agent prepared in Example 1;
[0042] Treatment 1: without adding the straw-degrading acidifying bacterial agent prepared in Example 1.
[0043] Treatment 2: use the straw-degrading acidifying bacterial agent prepared in Example 1.
Embodiment approach
[0044] Implementation method: Two groups of experiments were designed. One group of experiments used 660ml of bacterial agent, 20g of yellow storage corn stalks crushed to 1-2cm, and 180rpm shaking culture; the other group did not add bacterial agent, and ensured that other experimental conditions were the same.
[0045] The content of cellulose, hemicellulose and volatile organic acid was detected every day, and the degradation rate was calculated. The detection methods of cellulose, hemicellulose and volatile organic acids are based on literature: "Determination of cellulose_hemicellulose and lignin in corn straw", Wang Jinzhu, Wang Yuanxiu, Li Feng, Gao Yanhua, Xu Junqing, Yuan Jianguo, Shandong Food Fermentation , 2010.3 (total issue 158), cellulose degradation rate = decomposed cellulose amount / total cellulose amount, hemicellulose degradation rate = decomposed hemicellulose amount / total hemicellulose amount, lignin degradation rate = decomposed Decomposed lignin amount / t...
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