Composite bacterial coal biological flocculant and method for purifying slime water by same

A technology of biological flocculants and composite bacteria, applied in the direction of flocculation/sedimentation water/sewage treatment, etc., can solve complex problems, high production costs, difficult management, etc. Effect

Active Publication Date: 2013-01-16
ANHUI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current slime water treatment system can effectively realize the closed-circuit circulation of the slime water only by using flocculants for treatment. Since the slime water process is affected by many factor

Method used

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  • Composite bacterial coal biological flocculant and method for purifying slime water by same
  • Composite bacterial coal biological flocculant and method for purifying slime water by same
  • Composite bacterial coal biological flocculant and method for purifying slime water by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 obtains Aspergillus niger and Phanerochaete chrysosporium culture stoste and each part of culture stoste

[0028] 1. Obtain Aspergillus niger culture stock solution

[0029] Aspergillus niger medium formula: 20% potato juice 1000mL, glucose 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, vitamin B1 8mg, pH=6 (preparation of 20% potato juice: take 200g peeled potatoes, cut into small pieces, add 1000mL of water and boil for 20min, filter out the potato pieces, and make up the filtrate to 1000mL).

[0030] Based on the above-mentioned Aspergillus niger culture medium formula, obtain following 3 kinds of liquid culture medium:

[0031] Medium No. 1: 90% by volume of Aspergillus niger medium and 10% by volume of slime water were mixed.

[0032] Medium No. 2: Mix 70% by volume of Aspergillus niger medium and 30% by volume of slime water.

[0033] Medium No. 3: Mix 40% by volume of Aspergillus niger medium and 60% by volume of slime water.

[0034] Each culture me...

Embodiment 2

[0045] Example 2 Purification of coal slime water by composite bacterial strain coal bioflocculant

[0046] The flocculation effect of the coal bioflocculant in purifying slime water is measured by the flocculation rate, the higher the flocculation rate, the better the flocculation effect. The formula for calculating the flocculation rate is:

[0047]

[0048] In the formula: A o - OD of supernatant without adding coal bioflocculant 660 value;

[0049] A i -Supernatant OD after adding coal bioflocculant flocculation 660 value.

[0050] In order to ensure that the absolute amount of flocculation of coal slime particles is comparable, the total volume of each reaction system is kept at a fixed amount in the following examples.

[0051] The coal slime water used in the following examples is taken from the flotation tailing coal slime water of Huainan Wangfenggang Coal Preparation Plant. The relevant properties of slime water are shown in Table 2:

[0052] Table 2

[0...

example 1

[0058] 1) Get 90mL of uniformly stirred coal slime water, then add 2% by mass of CaCl 2 Coagulant aid 2mL, stir with a magnetic slow speed stirrer for 1min;

[0059] 2) Add 3mL of coal bioflocculant, add coal slime water to 100mL, adjust the pH value of coal slime water to 7 with a pen-type pH meter, and then stir slowly for 1min with a magnetic stirrer. The coal bioflocculant includes Aspergillus niger stock solution centrifugation Supernatant 1.5mL and Phanerochaete chrysosporium stock solution centrifuged supernatant 1.5mL, the active culture time of Aspergillus niger and Phanerochaete chrysosporium was 2 days.

[0060] 3) Stand still for 30 minutes to absorb the supernatant, measure the absorbance value of the supernatant at 660 nm with a UV-5100 ultraviolet spectrophotometer, and obtain a flocculation rate of 92.48%. When other conditions remain unchanged, the coal bioflocculant is replaced with 3ml Aspergillus niger stock solution centrifuged supernatant, and the floccu...

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Abstract

The invention relates to a composite bacterial coal biological flocculant which has the advantages of high flocculation rate, reduction in the limit of various factors on a flocculation effect and wider application range. The composite bacterial coal biological flocculant comprises aspergillus niger bacterial solution and phanerochaete chrysosporium bacterial solution at a volume ratio of 1 : 1, wherein the aspergillus niger bacterial solution and the phanerochaete chrysosporium bacterial solution respectively refer to culture stock solution obtained by culturing aspergillus niger and phanerochaete chrysosporium, the centrifugal supernate of the culture stock solution obtained by centrifuging the culture stock solution, broken solution obtained by breaking the culture stock solution or broken centrifugal supernate obtained by centrifuging the broken solution. The culture stock solution of the aspergillus niger and the culture stock solution of the phanerochaete chrysosporium are obtained by the following steps respectively: a, enrichment culture, b, adaptability culture, c, further enrichment culture, and d, activity culture.

Description

technical field [0001] The invention relates to a microbial preparation and its application, in particular to a coal biological flocculant of composite bacteria and its application. Background technique [0002] For a long time, the purification of slime water has been difficult to solve. The main reason is that with the improvement of the degree of mechanization of coal preparation, the proportion of fine coal in the slime water is increasing. Coal slime water concentrates the finest and most difficult-to-handle fine particles in raw coal. Because these particles have fine particle size, high ash content, high viscosity, and are difficult to settle, they are extremely difficult to treat with conventional sedimentation, recovery, and dehydration equipment. The current slime water treatment system can effectively realize the closed-circuit circulation of the slime water only by using flocculants for treatment. Since the slime water process is affected by many factors, the sl...

Claims

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Application Information

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IPC IPC(8): C02F1/56
Inventor 张东晨王涛侯志翔
Owner ANHUI UNIV OF SCI & TECH
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