110results about How to "Simplify design steps" patented technology

The invention mainly belongs to the technical field of higher organism genome editing, and particularly relates to an establishment method of a T7-RNA-polymerase-mediated CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing system. The method is based on the highly specific recognition principle for the T7 RNA polymerase and T7 promoter, and comprises the following steps: establishing T7 promoter-sgRNA, transforming the T7 promoter-sgRNA, a Cas9 expression plasmid and a T7 RNA polymerase expression plasmid into cells, and catalyzing the T7 promoter to promote the transcription of sgRNA by using the T7 RNA polymerase so as to guide the Cas9 to perform cutting at the target, thereby completing the gene editing process. By using the T7 promoter to express the sgRNA, the method simplifies the design steps of the CRISPR/Cas9 system, so that more people can quickly and easily research the interested gene locus by using the CRISPR/Cas9 gene editing tool.

The invention relates to a three-dimension design method based on a cable engineering information model. The three-dimension design method comprises the following steps: 1), according to main problems existing in city power cable and channel management at the present stage and defects of the conventional two-dimension design, which exist in actual engineering, determining an application field of the cable engineering information model; 2), determining demands in the three stages of cable engineering building, design and drawing and obtaining the achieving purpose of the cable engineering information model; 3), building the cable engineering information model by using a three-dimension design platform and according to a standard model base and regional digital model base; 4), carrying out three-dimension design on cable engineering according to the cable engineering information model and by combining cable structure parameters, a cable laying method and cable running parameters, and finally, obtaining a three-dimensional cable engineering designing scheme. Compared with the prior art, the three-dimension design method provided by the invention has the advantages of being intuitive and accurate, and advanced in platform and the like.

The invention discloses a single-ended input and differential output parallel dual-frequency low noise amplifier (LNA) and a design method thereof. The LNA can be applied to a digital TV, wireless communication and a navigation receiver and mainly comprises an input matching network, a main amplification circuit, an output network and a single-ended to differential circuit. The main amplification circuit adopts a cascode structure and has favorable reverse isolation degree; the input matching network enables the LNA to realize impedance matching and noise matching simultaneously in input stage and dual frequency ranges, and thus the noise performance of the circuit is improved at the same time of ensuring the maximum power transmission; and the singled-ended to differential circuit ensures that the LNA can be directly cascaded with a front-end antenna and a rear-end mixer circuit and easy to integrate. The LNA disclosed by the invention not only has a simple design, but also ensures that the system power consumption and area are reduced due to parallel dual-frequency receiving.

An application of graphene in polymerase chain reaction as a reinforcing agent relates to a graphene material. An improved Hummers method is utilized to synthesize graphite oxide, and hydrazine hydrate is utilized to synthesize graphene. A plasmid is used as a template to carry out polymerase chain reaction and multi-round polymerase chain reaction, the graphene can increase the specificity of the polymerase chain reaction within 14 mu gmL-1, and a target product with strong specificity can even be obtained through 8-round polymerase chain reaction. A clinical blood sample deoxyribonucleic acid (DNA) is taken as a template to carry out the polymerase chain reaction, and a polymerase chain reaction (PCR) reaction system which is added with the graphene can obtain a product with single specificity and can still obtain the target product with the strong specificity within a scope between 25 DEG C and primer annealing temperature. The amplification products which are added with the graphene do not influence the primer sequence and length through sequencing tests, and the graphene can be used as a polymerase chain reaction reinforcing agent with good performance for polymerase chain reaction.

The invention relates to a novel grid-connected photovoltaic power generation control method. Control refers to a power control link and a double-loop control link. In the power control link, the frequency and a reference signal of the voltage amplitude of a grid system are acquired according to drooping characteristics, the set drooping characteristics are automatically tracked through a PI controller, and then the purpose of controlling active power and reactive power to be output is achieved based on double-ring control of the active-disturbance-rejection technology. According to the grid-connected photovoltaic power generation control method based on the active-disturbance-rejection technology, designed steps are simple, an active-disturbance-rejection structure is strong in disturbance resistance ability, balance tracking of power under photovoltaic grid connected modes can be achieved, no-difference adjusting of the frequency and the voltage amplitude can be achieved, and operation reliability and safety of a photovoltaic grid-connected system are improved. The control method has general significance, is wide in application range and provides a new concept for controlling complex nonlinear controlled objects.

The invention relates to a charge-discharge-storage integrated station control method based on improved V2G (vehicle-to-grid) and priority scheduling. The charge-discharge-storage integrated station includes a charge station, an electrical changing station and a cascade station. The charge-discharge-storage integrated station control method includes the steps: 1) respectively taking every electricvehicle entering the charge-discharge-storage integrated station and the energy storage battery in the cascade station as one group of independent battery energy storage system, and predicting the real-time load distribution of the charge-discharge-storage integrated station; 2) determining the discharge lower limit of each group of battery energy storage system; 3) determining the charge-discharge priority of each substation in the charge-discharge-storage integrated station; and 4) according to the real-time power grid scheduling command and the predicted real-time load distribution, takingthe discharge lower limit of each group of battery energy storage system as the constraint condition, and according to the charge-discharge priority, determining the charge-discharge state of the each group of battery energy storage system. Compared with the prior art, the charge-discharge-storage integrated station control method based on improved V2G and priority scheduling can effectively avoid reducing the charging experience of the user because of over discharge of the electric vehicle which takes part in V2G, receives excess discharging and receives power grid scheduling, and achieves win-win of the electric vehicle user and the power grid.

The invention discloses a broadband directional microstrip patch antenna which is sequentially provided with a first medium layer, a microstrip feeder network arranged on the first medium layer, a second medium layer, a slit grounding surface arranged on the second medium layer, a third medium layer and a radiation patch array arranged on the third medium layer from bottom to top. The broadband directional microstrip patch antenna is simple to process and easy to assemble.

The invention provides a low-loss power distribution network optimization and reconfiguration method based on a genetic algorithm. According to the method, firstly, original progenitor chromosome populations are generated and encoded; whether lonely islands exist in the original progenitor chromosome populations or not is judged; chromosomes with the lonely islands are removed, and progenitor chromosome populations are obtained; the adaptation degrees of each chromosome in the progenitor chromosome populations are calculated, and sequencing is carried out according to the values of the adaptation degrees; whether the adaptation degrees meet design requirements or not is judged; if the adaptation degrees do not meet the design requirements, duplication, intersection and variation of the chromosome populations are carried out; the obtained chromosome populations are subjected to Elitism processing, i.e., before next iteration, the chromosomes with the best adaptation degree in the iterated progenitor chromosome populations are put into the next iteration; the iteration is repeated until iteration stopping conditions are met; and the optimum value is output. The low-loss power distribution network optimization and reconfiguration method based on the genetic algorithm provided by the invention has the advantages that Elitism is introduced; convergent results are more accurate; the problem of inconsistent convergent results of the genetic algorithm is solved; and the optimization efficiency is high.

The invention discloses a composite right/left handed transmission line and a design method thereof as well as a duplexer based on the transmission line. The composite right/left handed transmission line is a CRLH TL (composite right/left handed transmission line) unit A consisting of a complementary open single ring resonator pair and a micro-band gap or a CRLH TL unit B consisting of the complementary open single ring resonator pair, a fractal geometry structure and the micro-band gap. The design method comprises the following steps: 1. constructing an equivalent circuit model; 2.fitting a plurality of lumped circuit parameter values; 3. verifying the lumped circuit parameter values; and 4. confirming physical structure parameters. The duplexer comprises two CRLH TL units A or two CRLH TL units B. Seamless transition between left hand and right hand pass bands of the CRLH TL units adopted by the invention can be realized, the left hand and right hand pass bands are very easy to adjust so as to operate at a balance state, simultaneously the optimization and design method are simple and are convenient to realize, the design effect is good, additionally the composite duplexer has a compact structure, small volume, wide band width and good selection.

The invention provides a method for determining hole pattern parameters of a continuous mill by mechanical drawing. The method comprises the following steps of: filling parameters of a pipe blank and a rolled pipe blank and required parameters in a hole pattern parameter list according to a pass schedule; filling partial hole pattern design parameters and a regular relation in the hole pattern parameter list and calculating corresponding parameters; drawing a hole pattern according to the data by using a mechanical drawing method; labeling and measuring the size and angle of the mechanically drawn hole pattern; obtaining data such as a center point coordinate of each hole pattern element and a steel tube deformation area according to the size and angle; filling the parameters in the hole pattern parameter list and calculating data such as elongation; and obtaining data of the hole pattern parameter list. The invention has the advantages that the design method is verified by given hole pattern parameters of a continuous mill imported from abroad, and the data are completely consistent. The invention has the advantage that a complicated algorithm and design steps are simplified by the technical scheme.

The invention discloses a nonlinear system robust self-adaption tracking and controlling method based on unknown target traces. The nonlinear system robust self-adaption tracking and controlling method based on the unknown target traces comprises the following steps: step one, establishing a mathematic model of a multi-input and multi-output nonlinear system which has actuator faults; step two, establishing a model for estimating the ideal trace of the unknown target, wherein an estimated target trace obtained by the model is close to an ideal target trace; and step three, designing a robust self-adaption fault-tolerant controller. By the nonlinear system robust self-adaption tracking and controlling method based on unknown target traces, the estimated unknown target trace can be obtained, and is clear to the ideal target trace; 'virtual control gain' is subjected to matrix decomposition skillfully by the designed controller, so that limitation to conditions of a gain matrix in other control methods is greatly reduced; and finally, under the conditions that the system simultaneously has actuator faults, parameter uncertainty and external interferences and the tracked target trace is unknown, asymptotic tracked steady-state performance can still be obtained, and therefore, a closed-loop control system has robust self-adaption effect on an uncertain model and unknown interference.

The invention discloses a 12-point SCMA codebook design method based on a hexagonal planisphere and a SCMA system using the method. The codebook design method comprises the following steps: 1, allocating 12 constellation points on the end points of two embedded hexagons to form a total planisphere on a resource block; 2, splitting the total planisphere into 3 sub planispheres according to the principle that the minimum Euclidean distance on the same planisphere is the maximum, and separately expressing the 3 sub planispheres as S1, S2 and S3, wherein the size of each sub planisphere is 4; 3, generating a codebook of each user according to an incidence matrix F of the SCMA system and the sub planispheres used by different users on different resource blocks; and 4, performing power normalization on the codebook of each user so as to generate a system codebook. The codebook designed by the method has the advantages of low complexity, low error rate under high signal to noise ratio and convenient hardware implementation.

The invention relates to a wind power consumption ability analysis method. The method comprises the following steps: 1) data of a power system are acquired; 2) the start-up mode of the power system is determined, and the minimal start-up capacity of a non-wind turbine generator is determined according to the start-up mode of the power system; 3) the thermal stability limit and the transient stability limit of each main transmission channel and the maximal transmission capacity of each main transmission channel are calculated; 4) the maximal wind power output provided by each hour is calculated; 5) the wind power output based on network constraints is calculated; 6) the wind power accommodation space based on the system peaking capability is calculated and obtained; 7) the actual wind power output and the maximal transmission capacity of each main transmission channel are calculated, and the limited output of each main transmission channel in the power system is calculated respectively and correspondingly; 8) wind power generation is calculated, and the wind power limit is calculated; and 9) an abandoned wind power ratio is calculated. The method of the invention can intuitively and accurately represent the wind power consumption ability by the power system.

The invention relates to a self-adaptation control method for an electric vehicle for uncertain barycenter and unknown input saturation. The method comprises the following steps: 1) establishing a mathematical model for an electric vehicle system containing uncertain barycenter and longitudinal sliding/lateral sliding; 2) using a smooth functional approximation executor for saturating, considering an executor fault and applying to a state space expression; 3) designing and outputting a limited robust adaptive controller for controlling a motion track of the electric vehicle. According to the invention, the uncertain influence caused by the uncertain barycenter of the electric vehicle can be coped with; the control problem caused by the uncertain barycenter can be solved; the unknown executor nonlinear saturated characteristics can be smartly treated; the control problem caused by the asymmetrical saturation can be solved; when the executor is in fault, the electric vehicle still can move along the expected track; the motion track of geometric center position of the electric vehicle can be limited within a track scope which is given in advance, and meanwhile, the design steps of the controller are simplified and the controller is user-friendly.

The invention discloses a balance type band-pass filter with a harmonic suppression function, and solves the technical problem that the balance type band-pass filter with the harmonic suppression function, a simple structure and convenience in design and production is not available in the prior art. The filter comprises a port layer, a resonator layer and a ground layer, wherein the port layer comprises a first differential port and a second differential port; the resonator layer comprises a first resonator and a second resonator; the first resonator and the second resonator are both half-wavelength open-loop resonators; a differential feed port is formed in a voltage zero point of a resonator secondary harmonic and is in orthogonal coupling with the corresponding resonator to excite the resonator fundamental wave to form a passband, and to suppress secondary harmonic at the same time; and the open loop side of the first resonator and the open loop side of the second resonator are arranged at intervals so as to realize coupling between the resonators and to form a resonator third harmonic band-elimination structure. A stop band on a differential mode is expanded while broadband common-mode rejection is realized by the filter; and in addition, the design steps are quite simple and the performance is good.

The present invention discloses a resonance type gyro stability analysis method, which comprises the following three basic steps: (1) establishing the dynamic model of a resonance type gyro; (2) drawing the stability parameter region distribution map of the resonance type gyro; and (3) determining the stability parameter range of the resonance type gyro. According to the present invention, the coefficient of the resonance type gyro dynamic model is the rapid varying function of the time, and the vibration caused by the parameter change is named the parameter excitation characteristic equation; according to the stability analysis method of the resonance type gyro, the stability parameter region distribution map of the resonance type gyro is obtained by using the small parameter method based on the dynamic model, the two Lyapunov functions of the resonance type gyro dynamic model are established, and the stability of the gyro dynamic model is adopted as the conversion condition so as to achieve the parameter value range of the stable working of the gyro; and the method overcomes the influence of the parameter excitation on the stable working of the gyro, achieves the steady state output of the resonance type gyro, extends the application scope, and can further be used for the stability analysis of other resonance type sensors.

The invention discloses an ultra-wideband filter employing parallel resonators and having a band-stop characteristic and a function of restraining WLAN (Wireless Local Area Network) interference signals. A first feed network and a second feed network of the ultra-wideband filter have the identical structure and are arranged symmetrically relative to a gap between the two feed networks, a first transmission line and a fourth transmission line are respectively inserted into two ends of the gap. A metallized through-hole unit, the first transmission line and a second transmission line form a short circuit T-shaped resonator. A third transmission line and the fourth transmission line form an open circuit T-shaped resonator. The resonant frequency of the short circuit T-shaped resonator is at the low side of the frequency range of 3.1GHz to 10.6GHz, and the resonant frequency of the open circuit T-shaped resonator is at the high side of the frequency range of 3.1GHz to 10.6GHz. The ultra-wideband filter can not only couple signals within the pass band frequency range to the resonator, but also can be used to generate stop band at appointed frequency points. The ultra-wideband filter has the advantages of small size, good frequency selectivity, easy process and low cost.

The invention provides a manufacture method of a laser chip. The manufacture method includes growing a ridge waveguide layer and a sacrificial layer; etching the sacrificial layer and forming a convexstructure which defines an inter-ridge distance; settling a mask layer; etching the mask layer so as to expose the top end face of the sacrificial layer and remaining the mask layer side walls on twosides of the sacrificial layer; removing the convex structure by adopting a wet etching method; defining the double groove position and the width; etching the ridge waveguide layer; removing the masklayer side walls through etching and forming a double-ridge double-groove structure; and settling an electrode layer. According to the invention, when testing laser chips manufactured by adopting theabove manufacture method, two lasers can be tested separately. Secondary picking can be performed in unqualified chips of the first picking, so that chip scrap is reduced, chip yield rate is improvedand chip manufacture cost is reduced.

The invention provides a multi-grating AlGaN/GaN HETM small signal model and a reference extraction method. The multi-grating AlGaN/GaN HETM small signal model comprises a parasitic part and an intrinsic part; the parasitic part comprises grating capacitors Cpgi, Cpdi and Cgdi which are applicable for characterizing an additional parasitic capacitance effect generated by the intersection of multiple gratings and the interaction between the gratings and side source-drain electrodes. According to the multi-grating AlGaN/GaN HETM small signal model, for high-power large-current multi-grating large-sized devices, by additionally arranging the grafting capacitors Cpgi, Cpdi and Cgdi to characterize the additional parasitic capacitance effect generated by the intersection of the gratings and theinteraction between the gratings and the side source-drain electrodes, the characteristics of large-sized devices can be more accurately reflected, and the accuracy of the device model is improved.

The invention discloses a method for designing a stabilizer bar of an automotive suspension. The method comprises the steps of: (a) setting the installing position of the suspension stabilizer bar and the length and position data of a connecting rod according to predefined shapes and installing positions of all parts of the automotive suspension; (b) building a DMU model according to the set data to generate motion envelopes; (c) laminating the motion envelopes to obtain a maximum value, setting the shape of the suspension stabilizer bar, and obtaining a gap between the suspension stabilizer bar and the maximal motion envelope; (d) judging if any value of the obtained gap is greater than a predefined safety gap, if so, operating the step (e), if not, returning to the step (b) after resetting the installing position of the suspension stabilizer bar and the length and position data of the connecting rod; and (e) finishing the design. The invention further discloses a system for designing the stabilizer bar of the automotive suspension. The method and the system simplify the design steps of the suspension stabilizer bar, shorten the design period, and improve the shape design level of the suspension stabilizer bar.