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A crop straw decomposing bacterial agent and its use method and effect

A technology for crop straw and decomposing inoculum, which is applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc. It can improve crop quality, increase nutrient conversion rate, and reduce pests and diseases by solving problems such as short treatment time.

Active Publication Date: 2016-03-16
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Straw returning directly to the field is a project promoted in recent years. Straw returning machines are used for operation, which has a high degree of mechanization, short straw processing time, and long decay time in the soil, which affects plowing and rotary tillage operations and is not conducive to sowing. In recent years, often Burning the straw happened, which not only fails to achieve the purpose of straw resource utilization, but also pollutes the environment

Method used

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  • A crop straw decomposing bacterial agent and its use method and effect
  • A crop straw decomposing bacterial agent and its use method and effect
  • A crop straw decomposing bacterial agent and its use method and effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of media and strains

[0037] 1. Preparation of culture medium

[0038] 1. Potato and dextrose agar medium (PDA)

[0039] Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.

[0040] 2. Nutrient gravy agar

[0041] It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).

[0042] 3. Potassium bacteria medium

[0043] Yeast extract 0.4g, mannitol 10.0g, K 2 HPO 4 0.5g, MgSO 4 7H 2 O0.2g, FeCl 3 0.005g, CaCO 3 1.0g, 1000.0ml distilled water, pH7.0-7.2, add 15g agar when preparing solid medium.

[0044] 4. Solid fermentation medium

[0045] 80g wheat bran, 10g s...

Embodiment 2

[0058] Embodiment 2: (parts by weight)

[0059] A crop straw decomposing bacterial agent, prepared as follows (by weight):

[0060] Table 1 Composition of crop straw decomposing bacterial agent

[0061] name Phaneroderma chrysosporium Trichoderma chrysogreen Bacillus subtilis Bacillus peptiformis （1） 0.5 2.0 1.0 2.0 （2） 1.0 1.5 2.0 1.5 （3） 1.5 1.0 1.5 1.0 （4） 2.0 0.5 0.5 0.5

[0062] The number of effective viable bacteria is 6×10 8 piece / g;

Embodiment 3

[0064] Mutual antagonism experiment between strains

[0065] The activated Phaneroderma chrysosporium, Trichoderma chrysogenum, Bacillus subtilis and Bacillus peptone-like were subjected to the mutual antagonism experiment between strains, and they were combined in pairs, divided into 12 combinations in total, and each combination was repeated three times.

[0066] The steel ring method was used to carry out the antibacterial test, and the strain was cultured in the corresponding medium for 3-5 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibacteri...

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Abstract

The invention discloses a crop straw rot rotten bacterium and its application in straw biological reactors and fertilizers.The fungus is made of 0.5 to 2 parts of the yellow spores, 0.5 to 2 parts of yellow and green wooden mold, 0.5 to 2 copus of Bacillus of Bacillus and 0.5 to 2 parts of gluebacteria.Number 6 × 108 / g.The straw rot cooked bacterium is used in tomato straw biological reactor planting technology, with an average ground temperature of 18.3 ° C, an increase of 2.2 ° C from the control of the non -bacterial agent; the average shed temperature is 14.3 ° C, an increase of 2.0 ° C.2.17 times; the harvest period is 15 days in advance; the perurone output is 5881kg, which is 22.7%more than the control of the control. Compared with the control, there is less pests.The bacteria are used in wheat straw stacks, rot, fast decomposition, good softness, and good softening, straw is basically decomposed, and the fermentation time is shorter than the bacteria purchased by the application market.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms in processing crop straws, and relates to a crop straw decomposing bacterial agent and its application in straw bioreactors and stack rettings. Background technique [0002] At present, the production of crop straws in my country reaches 700-1 billion tons. Straws contain a large amount of organic matter, nitrogen, phosphorus, potassium and trace elements, and are one of the important organic fertilizer sources in agricultural production. Straw returning directly to the field is a project promoted in recent years. Straw returning machines are used for operation, which has a high degree of mechanization, short straw processing time, and long decay time in the soil, which affects plowing and rotary tillage operations and is not conducive to sowing. In recent years, often The occurrence of burning the straw will not only fail to achieve the purpose of straw resource utilization,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F11/08A01G1/00C12R1/885C12R1/125C12R1/07C12R1/645
CPCY02W30/40
Inventor 何宗均田阳梁海恬赵琳娜
Owner 天津市农业科学院
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