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Method for degrading rice straws by using oxalic acid and Mn2+ for accelerating catalysis of compound enzyme

A technology for catalytic degradation of rice straw, applied in pulping with acid/acid anhydride, textiles and paper, papermaking, etc., can solve the problems of low degradation rate of rice straw, large amount of acid and alkali, and small environmental pollution, etc., to achieve simple operation, Improved enzymatic hydrolysis efficiency and low operating costs

Inactive Publication Date: 2012-07-04
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many researches mainly use physical and chemical means to treat rice straw, including microwave radiation method and acid and alkali treatment method, etc., but this kind of method consumes a lot of energy, consumes a lot of acid and alkali, and has low recovery, which is easy to cause environmental pollution.
The other is the use of biotechnology to treat rice straw, the main focus of which is to use lignin degrading enzymes (such as lignin peroxidase (LiP), manganese peroxidase (MnP)) to catalyze the degradation of lignocellulose in rice straw , this kind of method has less environmental pollution and lower energy consumption, but since it is difficult for polymer substances such as LiP and MnP to enter the intact and undamaged rice straw, the degradation rate of rice straw is simply catalyzed by lignin degrading enzymes. lower

Method used

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  • Method for degrading rice straws by using oxalic acid and Mn2+ for accelerating catalysis of compound enzyme
  • Method for degrading rice straws by using oxalic acid and Mn2+ for accelerating catalysis of compound enzyme
  • Method for degrading rice straws by using oxalic acid and Mn2+ for accelerating catalysis of compound enzyme

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Embodiment 1

[0022] A kind of utilization oxalic acid and Mn of the present invention 2+ The method for promoting compound enzymes to catalyze the degradation of rice straw comprises the following steps:

[0023] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 60-mesh sieve for later use;

[0024] (2) Preparation of compound enzyme solution: add 2 mL of spores to every 200 mL of sterilized liquid culture medium at a concentration of 1.5×10 6 Phanerochaete chrysosporium spore suspension per mL, cultured with shaking at 30°C and 120 r / min for 6 days, and obtained compound enzyme solution (15 U / L LiP and 213 U / L MnP); liquid medium (1 L) formula is: glucose 10 g, ammonium tartrate 0.2 g, MgSO 4 0.71 g, Tween 80 0.5 mL, KH 2 PO 4 2.56 g, VB 1 0.01 g, sodium tartrate buffer 4.616 g, trace element solution 100 mL, benzyl alcohol 0.516 mL, H 2 O 899.484 mL, pH adjusted to 4.5;

[0025] (3) Catalytic degradation: Add the compound enzyme solution prepared i...

Embodiment 2

[0033] A kind of utilization oxalic acid and Mn of the present invention 2+ The method for promoting compound enzymes to catalyze the degradation of rice straw comprises the following steps:

[0034] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 100-mesh sieve for later use;

[0035] (2) Preparation of compound enzyme solution: Add 4 mL of spores to each 200 mL of sterilized liquid medium (the formula is the same as in Example 1) at a concentration of 2×10 6 Phanerochaete chrysosporium spore suspension per mL, cultured with shaking at 37°C and 150r / min for 8 days, and obtained the compound enzyme solution (20 U / L by precipitation, filter paper filtration, high-speed centrifugation and 0.45mm filter membrane filtration) LiP and 250 U / L of MnP);

[0036] (3) Catalytic degradation: Add the compound enzyme solution prepared in step (2) to 5kg of the straw powder obtained in step (1), and at the same time add oxalic acid solution with a concentrati...

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Abstract

The invention discloses a method for degrading rice straws by using oxalic acid and Mn2+ for accelerating the catalysis of a compound enzyme. The method comprises the following steps of: (1) perprocessing the straws: crushing the rice straws to obtain straw powder, and sifting for standby; (2) preparing a compound enzyme soltion:culturing phanerochaete chrysosporium in a liquid state to obtain the compound enzyme solution; and (3) carrying out catalytic degradation: adding the compound enzyme solution into the straw powder, meanwhile, adding an oxalic acid solution, a Mn2+ solution and a H2O2 solution, carrying out heat-preservation moisture-preservation standing, and finishing the catalytic degradation. The method for degrading the rice straws, disclosed by the invention, has the advantages that: the degrading speed of the compound enzyme to lignin is increased, the thermal energy consumption is lowered, meanwhile, the cost is low, the operation is simple, the operating cost is low, the method is clean and pollution-free, and thus, the industrial production can be achieved.

Description

technical field [0001] The invention relates to the technical field of biodegradation, in particular to a method of utilizing oxalic acid and Mn 2+ A method for promoting compound enzymes to catalyze the degradation of rice straw. Background technique [0002] Rice straw is an abundant renewable resource and one of the main crop straws. However, because the output of straw varies with the seasons, and the output is large, the value is low, the volume is large, and it is inconvenient to transport. The natural degradation process of straw is extremely slow, resulting in a large amount of straw being dumped directly into the environment in the form of accumulation and burning. The amount of straw is about 5×10 7 t~7×10 7 t, accounting for 10% to 15% of the total straw production, causing great environmental pollution and serious waste of material resources. [0003] Straw is composed of organic matter, a small amount of minerals and water. The organic matter is mainly ligni...

Claims

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Application Information

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IPC IPC(8): D21H11/12D21C5/00D21C3/04D21C3/22
Inventor 曾光明赵美花黄丹莲赖萃危臻黄超李宁杰许飘张辰
Owner HUNAN UNIV
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