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Ceriporialacerata and application thereof in preventing and treating crop fungus diseases

A technology of tearing cereus and antagonism, which is applied in the field of tearing new species of cereus, can solve the problems of poor biocontrol effect and limited number of biocontrol bacteria, and achieve low cost and rich biocontrol bacteria resources library, rapid growth effect

Active Publication Date: 2017-09-26
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited number of existing bio-control bacteria and the poor bio-control effect, the purpose of the present invention is to provide a new species of cereus spp. HG2011, which has a significant bio-control effect on crop fungal diseases

Method used

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  • Ceriporialacerata and application thereof in preventing and treating crop fungus diseases
  • Ceriporialacerata and application thereof in preventing and treating crop fungus diseases
  • Ceriporialacerata and application thereof in preventing and treating crop fungus diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening of bacterial strain HG2011

[0029] The litter layer of pine forest in Jinyun Mountain was collected, and lignin-decomposing fungi were screened out by dilution plate coating method.

Embodiment 2

[0031] Embodiment 2: Identification of bacterial strain HG2011

[0032] 1. Morphological observation of bacterial colonies

[0033] The above strain HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, the colony morphology was observed and recorded by taking pictures. Use an inoculation needle to pick the hyphae onto a glass slide, add 1 drop of lactic acid carbolic wool blue staining solution, cover with a cover glass and observe under a microscope. figure 1 The HG2011 bacterial strain obtained for the present invention has the following morphology under an optical microscope: the bacterial strain grows rapidly, the colony is large, fluffy, dry, opaque, fluffy and flocculent, the hyphae can spread and grow along the surface of the medium, and the colony is initially white , turning pale yellow ( figure 1 A). Reproductive hyphae are colorless without septa, thin-walled, frequently branched, interlaced ( figure 1 B); elliptic basidiospores ( figure 1 C)...

Embodiment 3

[0038] Embodiment 3: Determination of antibacterial properties

[0039] 1. Determination of extracellular enzymes

[0040] C. lacerate HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, along the edge of the colony, one bacterial block with a diameter of 6 mm was taken, inoculated in the center of chitin agar medium, and cultured at 25°C for 5 days. Under the action of chitin hydrolase, the milky white chitin is hydrolyzed into soluble small molecular substances, the milky white chitin disappears, and a colorless transparent area is formed around the colony (see Figure 4 A).

[0041] C. lacerate HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, along the edge of the colony, one bacterial block with a diameter of 6 mm was taken, inoculated in the center of cellulose Congo red solid medium, and cultured at 25°C for 5 days. Under the action of cellulase, cellulose is hydrolyzed into small molecular sugars, the red color formed by Cong...

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Abstract

The invention relates to a novel ceriporialacerata species and application thereof in preventing and treating crop fungus diseases. A preservation number of the novel ceriporialacerata species is CGMCC (China General Microbiological Culture Collection Center) No.13899, the novel ceriporialacerata species is classified and named as Ceriporia lacerate HG2011, and the strain has a nucleotide sequence in a C. lacerate HG2011 18SrDNA sequence list and belongs to Ceriporia of Phanerochaetaceae. A bacterial colony is white and in villous and flocculent state, and an appropriate growth temperature is 25 to 28 DEG C. The strain can secrete varieties of extracellular enzymes of chitinase, cellulase, beta-1,3-glucanase, protease and the like; fermentation broth of the strain has a stronger antagonism effect on varieties of plant pathogenic fungi; the strain can be applied to preventing and treating flue-cured tobacco and phytophthora capsici diseases and has larger application potential in an aspect of preventing and treating the plant pathogenic fungi.

Description

technical field [0001] The invention relates to a new species of cereus tearing fungus, and also relates to the application of the strain in the prevention and treatment of crop fungal diseases, belonging to the field of agricultural biocontrol microorganisms. Background technique [0002] Chemical pesticides are important means of agricultural production. In modern intensive agricultural production, large and frequent use of pesticides is required to prevent and control crop diseases. However, the long-term and large-scale application of pesticides has caused a series of production, environmental and health problems, such as enhanced drug resistance of bacteria, reduced drug efficacy, increased dosage, increased pesticide residues in the environment and agricultural products, affecting food safety, endangering human health, etc. . [0003] Fungal diseases account for 70% to 80% of crop diseases. At present, the main methods of disease prevention and control are breeding h...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 黄建国殷洁袁玲
Owner SOUTHWEST UNIVERSITY
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