Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of cereus tearing fungus and its application in the control of fungal diseases of crops

A technology for tearing cereus and plant fungal diseases, applied in the fields of application, fungi, microorganism-based methods, etc., can solve the problems of poor bio-control effect and limited number of bio-control bacteria, and achieve low cost and richness. Biocontrol bacteria resource bank, rapid growth effect

Active Publication Date: 2020-06-05
SOUTHWEST UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited number of existing bio-control bacteria and the poor bio-control effect, the purpose of the present invention is to provide a new species of cereus spp. HG2011, which has a significant bio-control effect on crop fungal diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of cereus tearing fungus and its application in the control of fungal diseases of crops
  • A strain of cereus tearing fungus and its application in the control of fungal diseases of crops
  • A strain of cereus tearing fungus and its application in the control of fungal diseases of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening of bacterial strain HG2011

[0029] The litter layer of pine forest in Jinyun Mountain was collected, and lignin-decomposing fungi were screened out by dilution plate coating method.

Embodiment 2

[0031] Embodiment 2: Identification of bacterial strain HG2011

[0032] 1. Morphological observation of bacterial colonies

[0033] The above strain HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, the colony morphology was observed and recorded by taking pictures. Use an inoculation needle to pick the hyphae onto a glass slide, add 1 drop of lactic acid carbolic wool blue staining solution, cover with a cover glass and observe under a microscope. figure 1 The HG2011 bacterial strain obtained for the present invention has the following morphology under an optical microscope: the bacterial strain grows rapidly, the colony is large, fluffy, dry, opaque, fluffy and flocculent, the hyphae can spread and grow along the surface of the medium, and the colony is initially white , turning pale yellow ( figure 1 A). Reproductive hyphae are colorless without septa, thin-walled, frequently branched, interlaced ( figure 1 B); elliptic basidiospores ( figure 1 C)...

Embodiment 3

[0038] Embodiment 3: Determination of antibacterial properties

[0039] 1. Determination of extracellular enzymes

[0040] C. lacerate HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, along the edge of the colony, one bacterial block with a diameter of 6 mm was taken, inoculated in the center of chitin agar medium, and cultured at 25°C for 5 days. Under the action of chitin hydrolase, the milky white chitin is hydrolyzed into soluble small molecular substances, the milky white chitin disappears, and a colorless transparent area is formed around the colony (see Figure 4 A).

[0041] C. lacerate HG2011 was inoculated on PDA solid medium, cultured at 25°C for 5 days, along the edge of the colony, one bacterial block with a diameter of 6 mm was taken, inoculated in the center of cellulose Congo red solid medium, and cultured at 25°C for 5 days. Under the action of cellulase, cellulose is hydrolyzed into small molecular sugars, the red color formed by Cong...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a new species of cereus tearing fungus and its application in the prevention and treatment of fungal diseases of crops. The preservation number is CGMCC No.13899, and the classification name is Ceriporia lacerate HG2011, the strain has C. lacerate Nucleotide sequence in HG2011 18S rDNA sequence listing. Belonging to the Phanerobacteriaceae ( Phanerochaetaceae ) cereus ( Ceriporia ). The colony is white, fluffy and flocculent; the suitable temperature for growth is 25~28℃. The strain can secrete a variety of extracellular enzymes such as chitinase, cellulase, β‑1,3‑glumerase and protease, and its fermentation broth has a strong antagonistic effect on a variety of plant pathogenic fungi, and is used in The control of flue-cured tobacco and capsicum phytophthora has great application potential in the control of plant pathogenic fungi.

Description

technical field [0001] The invention relates to a new species of cereus tearing fungus, and also relates to the application of the strain in the prevention and treatment of crop fungal diseases, belonging to the field of agricultural biocontrol microorganisms. Background technique [0002] Chemical pesticides are important means of agricultural production. In modern intensive agricultural production, large and frequent use of pesticides is required to prevent and control crop diseases. However, the long-term and large-scale application of pesticides has caused a series of production, environmental and health problems, such as enhanced drug resistance of bacteria, reduced drug efficacy, increased dosage, increased pesticide residues in the environment and agricultural products, affecting food safety, endangering human health, etc. . [0003] Fungal diseases account for 70% to 80% of crop diseases. At present, the main methods of disease prevention and control are breeding h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 黄建国殷洁袁玲
Owner SOUTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com