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Application of phanerochaete chrysosporium in degradation of tetracycline

A technology of P. chrysogenum and P. chrysogenum, which is applied to the application field of P. chrysosporium in degrading tetracycline, can solve the problems of secondary environmental pollution, no fundamental reduction of tetracycline content, inconspicuousness and the like.

Inactive Publication Date: 2016-03-23
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, from the reported literature, the existing methods still have the following problems: (1) using activated sludge adsorption to reduce the concentration of tetracycline in water, this method does not fundamentally reduce the content of tetracycline in the environment, and the residual The tetracycline in the excess sludge will bring the risk of secondary pollution to the environment; (2) The research on each process section in the sewage treatment plant shows that the primary treatment process of the sewage treatment plant has a negligible effect on the removal of antibiotic resistance genes ; The aerobic / anaerobic process in the secondary treatment process has a certain reduction effect on antibiotic resistance genes, but it is not obvious; (3) Using molecular methods, microorganisms have been found to be resistant to tetracyclines in activated sludge from sewage treatment plants. This provides a new idea for studying the degradation and removal of tetracycline from the perspective of microbiology, but the disadvantage of this method is that it cannot separate the live or pure microorganisms that can degrade tetracycline. no practical guidance

Method used

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  • Application of phanerochaete chrysosporium in degradation of tetracycline
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  • Application of phanerochaete chrysosporium in degradation of tetracycline

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Effect test

Embodiment 1

[0017] Embodiment 1 Phanerochaete chrysosporium is to the degradation of tetracycline in water body

[0018] PAD medium was used for subculture of Phanerochaete chrysosporium, and liquid medium (Kirk medium) was used for expanded culture. Phanerochaete chrysosporium was subcultured in PAD medium, and its spore powder was scraped off from PAD medium and placed in liquid medium for expanded culture for 48 hours. The trace amount of tetracycline in the water is carried out by simulating water distribution with tetracycline hydrochloride. Considering the influence of environment and other factors on the results, an orthogonal experiment was designed to investigate the effects of pH, temperature, Phanerochaete chrysosporium biomass and tetracycline concentration in water on the degradation effect. The concentration change of tetracycline before and after degradation was detected by ultraviolet / visible spectrophotometry, and the degradation calculation formula was as follows:

[0...

Embodiment 2

[0028] Application of Phanerochaete chrysosporium in the degradation of trace tetracyclines in water body in embodiment 2

[0029] From June to September 2015, the technical plan implemented in the laboratory is as follows:

[0030] 1. Stability test of tetracycline in liquid medium within 48h

[0031] In order to avoid the influence of tetracycline self-hydrolysis and the complexation and chelation of trace metal ions and vitamins in the culture medium on the detection results of Phanerochaete chrysosporium degradation of tetracycline, the experiment first investigated the tetracycline in the liquid medium within 48h. dynamic changes.

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Abstract

The invention relates to application of phanerochaete chrysosporium in degradation of tetracycline. According to the application, the microorganism species-phanerochaete chrysosporium which can degrade the tetracycline is discovered for the first time, and the content of the tetracycline in a water body can be easily, efficiently and quickly lowered through the action of biological metabolism and an enzyme system of the phanerochaete chrysosporium.

Description

technical field [0001] The invention relates to the field of microbiology and biodegradation, in particular to the application of Phanerochaete chrysosporium in degrading tetracycline. Background technique [0002] Antibiotics refer to a class of substances and their derivatives that are produced by bacteria, molds or other microorganisms during the reproduction process and can kill or inhibit other microorganisms. They are used to treat infections caused by sensitive microorganisms (usually bacteria or fungi). According to the statistics of the usage of antibiotics, in 1996, the amount of antibiotic feed additives used in the world accounted for 45.8% of the total amount of feed additives, and about 70% of the total output of antibiotics was used in animal husbandry; the veterinary drugs used in EU countries are mainly antibiotics and parasiticides Antibiotic veterinary drugs account for more than 70% of all veterinary drug consumption. The amount of antibiotics used in ea...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A23L5/20A23K10/00C12R1/01C02F101/38C02F101/34
CPCY02W10/37
Inventor 林毅青吴根义许振成邵继海陈安伟李晓翔王稼倩
Owner HUNAN AGRICULTURAL UNIV
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