Application of phanerochaete chrysosporium in degradation of tetracycline
A technology of P. chrysogenum and P. chrysogenum, which is applied to the application field of P. chrysosporium in degrading tetracycline, can solve the problems of secondary environmental pollution, no fundamental reduction of tetracycline content, inconspicuousness and the like.
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Embodiment 1
[0017] Embodiment 1 Phanerochaete chrysosporium is to the degradation of tetracycline in water body
[0018] PAD medium was used for subculture of Phanerochaete chrysosporium, and liquid medium (Kirk medium) was used for expanded culture. Phanerochaete chrysosporium was subcultured in PAD medium, and its spore powder was scraped off from PAD medium and placed in liquid medium for expanded culture for 48 hours. The trace amount of tetracycline in the water is carried out by simulating water distribution with tetracycline hydrochloride. Considering the influence of environment and other factors on the results, an orthogonal experiment was designed to investigate the effects of pH, temperature, Phanerochaete chrysosporium biomass and tetracycline concentration in water on the degradation effect. The concentration change of tetracycline before and after degradation was detected by ultraviolet / visible spectrophotometry, and the degradation calculation formula was as follows:
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Embodiment 2
[0028] Application of Phanerochaete chrysosporium in the degradation of trace tetracyclines in water body in embodiment 2
[0029] From June to September 2015, the technical plan implemented in the laboratory is as follows:
[0030] 1. Stability test of tetracycline in liquid medium within 48h
[0031] In order to avoid the influence of tetracycline self-hydrolysis and the complexation and chelation of trace metal ions and vitamins in the culture medium on the detection results of Phanerochaete chrysosporium degradation of tetracycline, the experiment first investigated the tetracycline in the liquid medium within 48h. dynamic changes.
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