Process for extracting polysaccharide from dendrobium officinale leaf according to enzymatic degradation method

A Dendrobium officinale polysaccharide and Dendrobium officinale technology, which is applied in fermentation and other directions, can solve the problem of low polysaccharide yield and achieve the effect of mild operating conditions and low pollution

Inactive Publication Date: 2018-05-01
贺州市星辉科技有限公司
View PDF9 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of low yield of polysaccharides extracted from Dendrobium officinale leaves by ordinary extraction methods, the present invention invented a process f

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for extracting polysaccharide from dendrobium officinale leaf according to enzymatic degradation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Get the leaves of Dendrobium officinale and dry them at a temperature of 50°C to a moisture content of <20%;

[0029] (2) Get 100g of dried Dendrobium officinale leaves and pulverize it, reflux 2 times with 95% ethanol solution with volume solubility of 10 times the weight, and merge the medicinal residues;

[0030] (3) Dry the above-mentioned combined medicinal residues, add distilled water according to the solid-liquid weight ratio of 1 / 30, and ultrasonically treat for 20 minutes at a power of 300W;

[0031] (4) Adjust the pH value to 6, then raise the temperature to 40°C in a water bath, then add cellulase and pectinase equivalent to 2% of the mass of medicinal residues respectively, and perform enzymatic hydrolysis for 2 hours, wherein the cellulose The enzyme is 500U / g, and the pectinase is 500U / g, which can destroy all kinds of cellulose in the cell tissue;

[0032] (5) The temperature of the water bath is raised to 50° C., the pH value is adjusted to 6.5, pa...

Embodiment 2

[0041] (1) Get the leaves of Dendrobium officinale and dry them at a temperature of 65°C to a moisture content of <20%;

[0042] (2) Get 500g of dried Dendrobium officinale leaves and pulverize it, reflux 3 times with 15 times the volume solubility of the weight of the ethanol solution of 95%, and merge the medicinal residues;

[0043] (3) Dry the above-mentioned combined medicinal residues, add distilled water according to the solid-liquid weight ratio of 1 / 25, and ultrasonically treat for 25 minutes at a power of 400W;

[0044] (4) Adjust the pH value to 6.5, then raise the temperature to 45°C in a water bath, then add cellulase equivalent to 2.5% of the mass of the dregs and pectinase equivalent to 2.5% of the mass of the dregs, and perform enzymatic hydrolysis for 2.5 hours, wherein the fiber Sulfase is 500U / g, pectinase is 500U / g, which can destroy all kinds of cellulose in cell tissue;

[0045] (5) The temperature of the water bath is raised to 55°C, the pH value is adj...

Embodiment 3

[0054] (1) Get the leaves of Dendrobium officinale and dry them at a temperature of 50-80°C to a moisture content of <20%;

[0055] (2) Get 1000g of dried Dendrobium officinale leaves and pulverize it, reflux 4 times with 20 times the volume solubility of the weight of the ethanol solution of 95%, and merge the medicinal residues;

[0056] (3) Dry the above-mentioned combined medicinal residues, add distilled water according to the solid-liquid weight ratio of 1 / 20, and ultrasonically treat for 30 minutes at a power of 500W;

[0057] (4) Adjust the pH value to 7, then raise the temperature to 50°C in a water bath, then add cellulase equivalent to 3% of the mass of medicinal residues and pectinase equivalent to 3% of the mass of medicinal residues, and perform enzymatic hydrolysis for 3 hours, wherein the cellulose The enzyme is 500U / g, and the pectinase is 500U / g, which can destroy all kinds of cellulose in the cell tissue;

[0058] (5) The temperature of the water bath is rais...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a process for extracting polysaccharide from a dendrobium officinale leaf according to an enzymatic degradation method. According to the process, a pure product of the dendrobium officinale polysaccharide is obtained by drying the dendrobium officinale leaf, crushing, backflowing with an organic solvent, collecting a dreg of a decoction, drying the dreg of the decoction, adding distilled water to dissolve, conducting ultrasonic treatment, conducting enzymolysis by using cellulase, pectinase and papain, heating to denature the enzyme, centrifuging, filtering, decoloringa filtrate by using an activated carbon, filtering and collecting the filtrate again, concentrating in vacuum, adding an organic solvent to conduct alcoholysis, filtering and collecting a precipitateagain, airing to obtain a crude product of the dendrobium officinale polysaccharide, adding distilled water to dissolve, dialyzing with a nanofilm, adding the organic solvent to conduct alcoholysis, filtering and collecting a precipitate again, and finally freeze-drying. The yield of the prepared dendrobium officinale polysaccharide is high, the operation condition of the enzymatic method is relatively mild, and the process has the advantages of high efficiency, energy saving, low pollution and the like.

Description

technical field [0001] The invention relates to a method for extracting components of dendrobium candidum, in particular to a technique for extracting polysaccharides from dendrobium candidum by using an auxiliary technology of enzyme degradation method, and belongs to the field of deep processing of dendrobium candidum. Background technique [0002] Dendrobium officinale is a perennial herb of the family Dendrobium genus. Dendrobium officinale is a very rare and precious Chinese medicinal material recorded in the Pharmacopoeia of the People's Republic of China. Dendrobium officinale is sweet in nature and slightly cold. Meridians, promoting body fluid and benefiting the stomach, clearing away heat and nourishing yin, nourishing the lungs and relieving cough, benefiting the kidney and improving eyesight. It has the effects of immune regulation and anti-aging. [0003] In recent years, Dendrobium officinale has been processed into Fengdou in large quantities, and Fengdou onl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/04C12P19/14
Inventor 孙炜炜黄鹏腾刘星韦武昌朱思思
Owner 贺州市星辉科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap