Process for extracting polysaccharide from dendrobium officinale leaf according to enzymatic degradation method

Abstract

The invention discloses a process for extracting polysaccharide from a dendrobium officinale leaf according to an enzymatic degradation method. According to the process, a pure product of the dendrobium officinale polysaccharide is obtained by drying the dendrobium officinale leaf, crushing, backflowing with an organic solvent, collecting a dreg of a decoction, drying the dreg of the decoction, adding distilled water to dissolve, conducting ultrasonic treatment, conducting enzymolysis by using cellulase, pectinase and papain, heating to denature the enzyme, centrifuging, filtering, decoloringa filtrate by using an activated carbon, filtering and collecting the filtrate again, concentrating in vacuum, adding an organic solvent to conduct alcoholysis, filtering and collecting a precipitateagain, airing to obtain a crude product of the dendrobium officinale polysaccharide, adding distilled water to dissolve, dialyzing with a nanofilm, adding the organic solvent to conduct alcoholysis, filtering and collecting a precipitate again, and finally freeze-drying. The yield of the prepared dendrobium officinale polysaccharide is high, the operation condition of the enzymatic method is relatively mild, and the process has the advantages of high efficiency, energy saving, low pollution and the like.

Description

technical field

[0001] The invention relates to a method for extracting components of dendrobium candidum, in particular to a technique for extracting polysaccharides from dendrobium candidum by using an auxiliary technology of enzyme degradation method, and belongs to the field of deep processing of dendrobium candidum. Background technique

[0002] Dendrobium officinale is a perennial herb of the family Dendrobium genus. Dendrobium officinale is a very rare and precious Chinese medicinal material recorded in the Pharmacopoeia of the People's Republic of China. Dendrobium officinale is sweet in nature and slightly cold. Meridians, promoting body fluid and benefiting the stomach, clearing away heat and nourishing yin, nourishing the lungs and relieving cough, benefiting the kidney and improving eyesight. It has the effects of immune regulation and anti-aging.

[0003] In recent years, Dendrobium officinale has been processed into Fengdou in large quantities, and Fengdou onl...

Images