Extracting method for pleurotus eryngii polysaccharide

Abstract

The invention discloses an extracting method for pleurotus eryngii polysaccharide, and belongs to the technical field of plant active ingredient extraction. The extracting method comprises the following steps: firstly, separating micromolecule components to the greatest extent by taking a low-concentration alcohol solution as a solvent, and supplementing with ultrasonic treatment; and then, extracting by taking water as a solvent and adopting an ultrasonic-inner boiling method and precipitating pleurotus eryngii polysaccharide from the high-concentration alcohol solution. Compared with conventional methods such as a water extraction method and an alcohol extraction method, the extracting speed and the extracting rate are obviously increased; the pleurotus eryngii polysaccharide yield is higher than or equal to 11% which is improved by 17-59% in comparison with that of the conventional method; and moreover, the activity of the pleurotus eryngii polysaccharide is kept to the greatest extent, so that the whole extracting process is short in consumed time, is high in yield, is less in impurities and is low in cost.

Description

technical field

[0001] The invention belongs to the technical field of extraction of plant active ingredients, and in particular relates to an extraction method of Pleurotus eryngii polysaccharide. Background technique

[0002] Pleurotus eryngii ( Pleurotuseryngii ) is a rare edible mushroom recommended by the Food and Agriculture Organization of the United Nations, and has become the second largest industrialized cultivar after Flammulina velutipes in my country. The polysaccharide contained in Pleurotus eryngii is its main active ingredient, which has certain anti-oxidation, anti-fatigue, antibacterial and anti-tumor effects. At present, there have been many studies on the extraction methods of Pleurotus eryngii polysaccharides. Fan Junmin and others used the water bath extraction method to extract, and found that the best conditions were extraction temperature 81°C, extraction time 3.4h, solid-liquid ratio 1g:27mL, and extraction once. The yield of polysaccharide was 8....