Extracting method for pleurotus eryngii polysaccharide

A technology of Pleurotus eryngii polysaccharide and extraction method, which is applied in the field of extraction of plant active ingredients, can solve the problems of high energy consumption, low extraction rate, and long extraction time, and achieve the effects of high yield, improved extraction rate, and shortened extraction time

Inactive Publication Date: 2016-07-20
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current extraction methods of Pleurotus eryngii polysaccharides have problems such as long extraction time, high energy consumption and low extrac

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for extracting pleurotus eryngii polysaccharides, comprising the following steps:

[0018] (1) Ultrasonic infiltration: According to the material-liquid ratio of 1:2g / mL, add ethanol solution with a mass concentration of 30% to 170 mesh Pleurotus eryngii powder, and treat it under 200W ultrasonic wave for 33min, so that Pleurotus eryngii powder is covered with ethanol solution full infiltration;

[0019] (2) Ultrasonic-internal boiling method extraction: After the infiltration, put the material obtained in step (1) into the ultrasonic circulation extractor, and add hot water at 100°C for extraction according to the material-liquid ratio of 1:25g / mL, and the extraction time 6.5min, ultrasonic power 550W; vacuum filtration after extraction, centrifugation and concentration of the filtrate;

[0020] (3) Alcohol precipitation: add ethanol solution with a mass concentration of 92% to the concentrated filtrate, let it stand for 15 hours, remove the supernatant, and ...

Embodiment 2

[0023] A method for extracting pleurotus eryngii polysaccharides, comprising the following steps:

[0024] (1) Ultrasonic infiltration: According to the material-liquid ratio of 1:5g / mL, add ethanol solution with a mass concentration of 50% to 200 mesh Pleurotus eryngii powder, and treat it under the action of 250W ultrasonic wave for 22min, so that the Pleurotus eryngii powder is covered with ethanol solution full infiltration;

[0025] (2) Ultrasonic-internal boiling method extraction: After the infiltration, put the material obtained in step (1) into an ultrasonic circulation extractor, and add hot water at 85°C according to a material-liquid ratio of 1:15g / mL for extraction. The extraction time 9.5min, ultrasonic power 350W; vacuum filtration after extraction, centrifugation and concentration of the filtrate;

[0026] (3) Alcohol precipitation: add ethanol solution with a mass concentration of 98% to the concentrated filtrate, let it stand for 13 hours, remove the superna...

Embodiment 3

[0029] A method for extracting pleurotus eryngii polysaccharides, comprising the following steps:

[0030] (1) Ultrasonic infiltration: According to the material-liquid ratio of 1:4g / mL, add ethanol solution with a mass concentration of 40% to 230 mesh Pleurotus eryngii powder, and treat it under 280W ultrasonic wave for 30min, so that the Pleurotus eryngii powder is covered with ethanol solution full infiltration;

[0031] (2) Ultrasonic-internal boiling method extraction: After the infiltration, put the material obtained in step (1) into an ultrasonic circulation extractor, and add hot water at 90°C according to the material-liquid ratio of 1:20g / mL for extraction, and the extraction time 8.0min, ultrasonic power 450W; vacuum filtration after extraction, centrifugation and concentration of the filtrate;

[0032] (3) Alcohol precipitation: add ethanol solution with a mass concentration of 95% to the concentrated filtrate, let it stand for 14 hours, remove the supernatant, an...

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Abstract

The invention discloses an extracting method for pleurotus eryngii polysaccharide, and belongs to the technical field of plant active ingredient extraction. The extracting method comprises the following steps: firstly, separating micromolecule components to the greatest extent by taking a low-concentration alcohol solution as a solvent, and supplementing with ultrasonic treatment; and then, extracting by taking water as a solvent and adopting an ultrasonic-inner boiling method and precipitating pleurotus eryngii polysaccharide from the high-concentration alcohol solution. Compared with conventional methods such as a water extraction method and an alcohol extraction method, the extracting speed and the extracting rate are obviously increased; the pleurotus eryngii polysaccharide yield is higher than or equal to 11% which is improved by 17-59% in comparison with that of the conventional method; and moreover, the activity of the pleurotus eryngii polysaccharide is kept to the greatest extent, so that the whole extracting process is short in consumed time, is high in yield, is less in impurities and is low in cost.

Description

technical field [0001] The invention belongs to the technical field of extraction of plant active ingredients, and in particular relates to an extraction method of Pleurotus eryngii polysaccharide. Background technique [0002] Pleurotus eryngii ( Pleurotuseryngii ) is a rare edible mushroom recommended by the Food and Agriculture Organization of the United Nations, and has become the second largest industrialized cultivar after Flammulina velutipes in my country. The polysaccharide contained in Pleurotus eryngii is its main active ingredient, which has certain anti-oxidation, anti-fatigue, antibacterial and anti-tumor effects. At present, there have been many studies on the extraction methods of Pleurotus eryngii polysaccharides. Fan Junmin and others used the water bath extraction method to extract, and found that the best conditions were extraction temperature 81°C, extraction time 3.4h, solid-liquid ratio 1g:27mL, and extraction once. The yield of polysaccharide was 8....

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 赖谱富陈君琛杨艺龙翁敏劼李怡彬
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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